ID A0A091M842_CARIC Unreviewed; 855 AA.
AC A0A091M842;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 {ECO:0000256|ARBA:ARBA00039538};
DE EC=2.8.2.8 {ECO:0000256|ARBA:ARBA00012979};
DE AltName: Full=Glucosaminyl N-deacetylase/N-sulfotransferase 1 {ECO:0000256|ARBA:ARBA00042929};
DE Flags: Fragment;
GN ORFNames=N322_03735 {ECO:0000313|EMBL:KFP67973.1};
OS Cariama cristata (Red-legged seriema).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Cariamiformes; Cariamidae; Cariama.
OX NCBI_TaxID=54380 {ECO:0000313|EMBL:KFP67973.1, ECO:0000313|Proteomes:UP000054116};
RN [1] {ECO:0000313|EMBL:KFP67973.1, ECO:0000313|Proteomes:UP000054116}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_N322 {ECO:0000313|EMBL:KFP67973.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3'-phosphoadenylyl sulfate + alpha-D-glucosaminyl-[heparan
CC sulfate](n) = adenosine 3',5'-bisphosphate + 2 H(+) + N-sulfo-alpha-
CC D-glucosaminyl-[heparan sulfate](n); Xref=Rhea:RHEA:21980, Rhea:RHEA-
CC COMP:9830, Rhea:RHEA-COMP:14602, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58339, ChEBI:CHEBI:58343, ChEBI:CHEBI:58388,
CC ChEBI:CHEBI:140572; EC=2.8.2.8;
CC Evidence={ECO:0000256|ARBA:ARBA00036125};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21981;
CC Evidence={ECO:0000256|ARBA:ARBA00036125};
CC -!- PATHWAY: Glycan metabolism; heparan sulfate biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005093}.
CC -!- PATHWAY: Glycan metabolism; heparin biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004841}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000256|ARBA:ARBA00037848}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00037848}.
CC -!- SIMILARITY: Belongs to the sulfotransferase 1 family. NDST subfamily.
CC {ECO:0000256|ARBA:ARBA00010420}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KK525488; KFP67973.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A091M842; -.
DR UniPathway; UPA00756; -.
DR UniPathway; UPA00862; -.
DR Proteomes; UP000054116; Unassembled WGS sequence.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0015016; F:[heparan sulfate]-glucosamine N-sulfotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0019213; F:deacetylase activity; IEA:UniProt.
DR GO; GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0030210; P:heparin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR021930; Heparan_SO4_deacetylase.
DR InterPro; IPR037359; NST/OST.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000863; Sulfotransferase_dom.
DR PANTHER; PTHR10605:SF30; BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE 1; 1.
DR PANTHER; PTHR10605; HEPARAN SULFATE SULFOTRANSFERASE; 1.
DR Pfam; PF12062; HSNSD; 1.
DR Pfam; PF00685; Sulfotransfer_1; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR637359-3};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000054116};
KW Transferase {ECO:0000313|EMBL:KFP67973.1}.
FT DOMAIN 58..488
FT /note="Heparan sulphate-N-deacetylase"
FT /evidence="ECO:0000259|Pfam:PF12062"
FT DOMAIN 578..831
FT /note="Sulfotransferase"
FT /evidence="ECO:0000259|Pfam:PF00685"
FT ACT_SITE 587
FT /note="For sulfotransferase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-1"
FT BINDING 685
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 790
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 806..810
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT DISULFID 791..801
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-3"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KFP67973.1"
FT NON_TER 855
FT /evidence="ECO:0000313|EMBL:KFP67973.1"
SQ SEQUENCE 855 AA; 98417 MW; 52BCC4BE67D699C3 CRC64;
LFVFCLLSVF VSAYYLYGWK RGLEPSGDVV GPDCDEPKVA PSRLLPLKTL KVVDSSRTDP
LVLVFVESLY SQLGQEIVAI LESSRFKYRT EIAPGKGDMP TLTDKDRGRF ALIIYENILK
YVNLDAWNRE LLDKYCVEYG VGIIGFFKAN ENSLLSAQLK GFPLFLHSNL ALKDCSINPK
SPLLYITRPS EVEKGVLPGE DWTVFQSNHS TYEPVLLAKT KSAESIPHMS VDAALHTTVM
QDLGLHDGIQ RVLFGNNLNF WLHKLVFVDS VSFLTGKRLS LPLDRYILVD IDDIFVGKEG
TRMKVEDVKA LFDTQNELRT HIPNFTFNLG YSGKFFHTGT DAEDEGDDLL LSYVREFWWF
PHMWSHMQPH LFHNQSVLAE QMTLNKKFAV EHGIPTDMGY AVAPHHSGVY PVHVQLYEAW
KQVWSIKVTS TEEYPHLKPA RYRRGFIHNG IMVLPRQTCG LFTHTIFYNE YPGGSSELDK
IINGGELFLT VLLNPISIFM THLSNYGNDR LGLYTFKHLV RFLNSWTNLK LQTLPPVQLA
QKYFQIFSEE KDPLWQDPCE DKRHKDIWSK EKTCDRFPKL LIIGPQKTGT TALYLFLGMH
PDLSSNYPSS ETFEEIQFFN GHNYHKGIDW YMEFFPIPSN TTSDFYFEKS ANYFDSEVAP
RRAAALLSKA KVITILINPA DRAYSWYQHQ RAHDDPVALK YTFHEVITAG PEAAPKLRTL
QNRCLVPGWY ATHIERWLNS YHANQILVLD GKLLRTEPAK VMETVQKFLG VTNFIDYHKT
LAFDPKKGFW CQLLDGGKTK CLGKSKGRKY PEMDSDSRAF LRDYYRDHNI ELSKLLYKMG
QTLPTWLREE LQSTR
//
