ID A0A091P1S7_HALAL Unreviewed; 1504 AA.
AC A0A091P1S7;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
DE Flags: Fragment;
GN Name=Agl {ECO:0000313|EMBL:NWZ58108.1};
GN ORFNames=HALALB_R01368 {ECO:0000313|EMBL:NWZ58108.1}, N329_10863
GN {ECO:0000313|EMBL:KFQ01214.1};
OS Haliaeetus albicilla (White-tailed sea-eagle) (Falco albicilla).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Accipitriformes; Accipitridae;
OC Accipitrinae; Haliaeetus.
OX NCBI_TaxID=8969 {ECO:0000313|EMBL:KFQ01214.1, ECO:0000313|Proteomes:UP000054379};
RN [1] {ECO:0000313|EMBL:KFQ01214.1, ECO:0000313|Proteomes:UP000054379}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_N329 {ECO:0000313|EMBL:KFQ01214.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:NWZ58108.1, ECO:0000313|Proteomes:UP000585422}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OUT-0040 {ECO:0000313|EMBL:NWZ58108.1};
RC TISSUE=Blood {ECO:0000313|EMBL:NWZ58108.1};
RA Zhang G.;
RT "Bird 10,000 Genomes (B10K) Project - Family phase.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
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DR EMBL; KK649888; KFQ01214.1; -; Genomic_DNA.
DR EMBL; VZSQ01000254; NWZ58108.1; -; Genomic_DNA.
DR Proteomes; UP000054379; Unassembled WGS sequence.
DR Proteomes; UP000585422; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Reference proteome {ECO:0000313|Proteomes:UP000054379};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 4..88
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 94..523
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 668..946
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1045..1499
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KFQ01214.1"
FT NON_TER 1504
FT /evidence="ECO:0000313|EMBL:KFQ01214.1"
SQ SEQUENCE 1504 AA; 170941 MW; 30A2CD122BA9D641 CRC64;
GFELQFRLGP TLQGKPVTVY TNYPASGEVF DRHKFRTLSW HNPTGKEDDS DKYCKLDLQI
SGSYQYYFSL GNEKSGGGYI VVDPILHVGA DNHVLPLDCV TLQTFLAKCL GPFHEWEDRL
KVAKETGYNM IHFTPLQKLG LSRSCYSLAD QLEVNPEFSS HNKKCTWSDI GALVEKMKNE
WNMLCITDVV YNHTATNSEW LRMHPECGYN LVNSPHLKPA WVLDRALWHL TCMVADGKYA
AKGVPSLIEN DHHLNCIRKL IWEDVYPKIK LWEFFQVDVN KAVQQFKSLL TEGKVSTKSD
PNEHLQIVQD PDYRRFGCTV DMNIALATFI PHSNGPAAIE ECCNWFRKRI EELNAEQYRQ
TNHHQEQAVN CLVGTVVYER LAGDGPKLGP ISRKNPLVTR YFTYPFKELT VEEEEAMIHQ
PDKACYFMAH NGWVMGDDPL RNFAEPGSNV YLRRELICWG DSVKLRYGNK PEDCPYLWAH
MKKYTEITAK YFHGVRLDNC HSTPIHVAEY MLDTARKLRA DLYVVAELFT GNEELDNIFV
NRLGITSLIR EAMTAYNSHE EGRLVYRFGG EPVGSFVQPR LRPLMPAIAH ALFMDITHDN
ECPIQHRSAY DALPSAMIVS MACCATGSTK GYDELVPHQI SVVSEERFYA KWNPATHLTS
GEVNFQTGIL AGRLAINRLH QELGAKGFNQ ARSEDQVDED IVAVTRHCPN THQSVVAVSR
TAFRDPKTSF YSKEVPEMCI PGKIEEVVLE ARTIERNASP YKKDERFING LPNFTMELRE
HIQIKDSKII KQAGTAIKGP NEFVQEIEFE KLTPGSVIVF RVSLDPKAQE AVGVLRNHLI
QFSPHFKSGS LPDDHSAPIL KTLFSSIASK LTLADLNQVL YRCEAEEQED GGGCYNIPNW
SPLKYAGLQG LMSVMADIRP KNDLGHPFCD NLRSGDWMID YVSNRLISRA GACAEVGKWL
KAMFVYLKRI PRYLIPCYFD AILVGAYTTL LDVGWHHMSS FVQNGSTFVK HLSLGSVQMC
GIGKYSCLPD LSPSLHDVPY RLNEITNEKE QCCVSLAAGL PHFSSGIFRS WGRDTFIALR
GLMLVTGRYL EARNIILAFG GTLRHGLIPN LLGQGTHARY NCRDAVWWWL QCIQDYCKIV
PNGLDILRCP VSRMYPRDDS SPQPAGTMDQ PLYEVIQEAM QRHMEGINFR ERNAGPQIDQ
NMRDEGFNVT AGVDNETGFV FGGNRFNCGT WMDKMGESDR ARNRGIPATP RDGSAVEIVG
LCKSAVRWLL DLSGKNVFPF RGVTVKRHEK EETITYDEWN RKIQGHFERL FFVSENPADP
NEKHPNLVHK RGIYKDSYGA SSPWCDYQLR PNFTIAMVVA PELFTPERAW KALQIAEEKL
LGPLGMKTLD PDDMVYCGVY DNALDNDNYN VARGFNYHQG PEWLWPIGYF LRAKLYFSKL
IGPEIYAKTV VMIKNVLSRH YVHLERSSWK GLPELTNENG QYCPFSCETQ AWSIGVILEI
LYDL
//
