ID A0A091SU51_NESNO Unreviewed; 617 AA.
AC A0A091SU51;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Galactocerebrosidase {ECO:0000256|ARBA:ARBA00019657};
DE EC=3.2.1.46 {ECO:0000256|ARBA:ARBA00012657};
DE AltName: Full=Galactosylceramidase {ECO:0000256|ARBA:ARBA00033098};
DE Flags: Fragment;
GN ORFNames=N333_06877 {ECO:0000313|EMBL:KFQ46522.1};
OS Nestor notabilis (Kea).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Psittaciformes; Psittacidae; Nestor.
OX NCBI_TaxID=176057 {ECO:0000313|EMBL:KFQ46522.1, ECO:0000313|Proteomes:UP000053840};
RN [1] {ECO:0000313|EMBL:KFQ46522.1, ECO:0000313|Proteomes:UP000053840}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_N333 {ECO:0000313|EMBL:KFQ46522.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a D-galactosylceramide + H2O = an N-acyl-sphingoid base + D-
CC galactose; Xref=Rhea:RHEA:43412, ChEBI:CHEBI:4139, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:36498, ChEBI:CHEBI:83273;
CC Evidence={ECO:0000256|ARBA:ARBA00023982};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43413;
CC Evidence={ECO:0000256|ARBA:ARBA00023982};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-galactosyl-(1<->1)-sphing-4-enine + H2O = D-galactose +
CC sphing-4-enine; Xref=Rhea:RHEA:43908, ChEBI:CHEBI:4139,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:57756, ChEBI:CHEBI:57934;
CC Evidence={ECO:0000256|ARBA:ARBA00023423};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43909;
CC Evidence={ECO:0000256|ARBA:ARBA00023423};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 59 family.
CC {ECO:0000256|ARBA:ARBA00005637}.
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DR EMBL; KK934445; KFQ46522.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A091SU51; -.
DR Proteomes; UP000053840; Unassembled WGS sequence.
DR GO; GO:0004336; F:galactosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006683; P:galactosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR049162; GH59_C.
DR InterPro; IPR049161; GH59_cat.
DR InterPro; IPR001286; Glyco_hydro_59.
DR InterPro; IPR035394; Glyco_hydro_59_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR15172; GALACTOCEREBROSIDASE; 1.
DR PANTHER; PTHR15172:SF1; GALACTOCEREBROSIDASE; 1.
DR Pfam; PF02057; Glyco_hydro_59; 1.
DR Pfam; PF21708; Glyco_hydro_59_C; 1.
DR Pfam; PF17387; Glyco_hydro_59M; 1.
DR PRINTS; PR00850; GLHYDRLASE59.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000053840};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Sphingolipid metabolism {ECO:0000256|ARBA:ARBA00022919}.
FT DOMAIN 1..284
FT /note="Glycosyl hydrolase family 59 catalytic"
FT /evidence="ECO:0000259|Pfam:PF02057"
FT DOMAIN 292..407
FT /note="Glycosyl hydrolase family 59 central"
FT /evidence="ECO:0000259|Pfam:PF17387"
FT DOMAIN 443..617
FT /note="Glycosyl hydrolase family 59 C-terminal lectin"
FT /evidence="ECO:0000259|Pfam:PF21708"
FT ACT_SITE 133
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601286-50"
FT ACT_SITE 209
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR601286-50"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KFQ46522.1"
FT NON_TER 617
FT /evidence="ECO:0000313|EMBL:KFQ46522.1"
SQ SEQUENCE 617 AA; 70116 MW; 786C6BCE5C1C90C4 CRC64;
ATSRLLVNYQ EPYRSQILDY LFKPNFGASL HILKVEIGGD GQSTDGTEPS HMHYENDENY
FRGYEWWLMK EAKKRNPKIK LIGLPWTFPA WIGKGENWPY DYPDVTAYYI VSWILGAKHY
HDLDIDYVGI WNERAFNSKY IKLLRYTLDK HGLQQVRIIA SDRLWEPISF VLLLDSELHG
VVDAIGAHYP GTKTVPNALL TKKKLWSSED YSTFNDEVGA GCWARILNQN YVNGNMTSTI
AWNLVASYYE ELPFGRCGLM TAQEPWSGHY KVEAPIWITA HTTQFTQPGW SYLQVDGHLK
GGGSFVALTD GLGNLTIVIE TMTYNHSQCI RPPLPHFTVT PQRATFYLKG SFYVVKTLQV
WHSRLDFESG RSSLFQQLHP VKVWKGSFSL DLNMDEVYTL TTLKTGQKCG YPEPPPPQPF
PSNYKDDFNI RNPPFSEAPN FADQTGVFEY FINASDPGDH VFTLRQVVVQ RPITWASDAD
QTISLIGNFM WVNMTVTCDI YIEKQRDGGV FVAGRVDNGG IYVRRTKGVF FWVFADGTYR
VTGDLAGEEI LMKGLSGVRD NVWHTLTLSI QGTSASGMLN GYPLWENVTI SQPSNGWAAV
GTRSFEFAQF DNFHVEA
//
