ID A0A093IQJ1_DRYPU Unreviewed; 649 AA.
AC A0A093IQJ1;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|PROSITE-ProRule:PRU00958};
DE EC=2.1.1.216 {ECO:0000256|PROSITE-ProRule:PRU00958};
DE Flags: Fragment;
GN ORFNames=N307_12458 {ECO:0000313|EMBL:KFV68986.1};
OS Dryobates pubescens (Downy woodpecker) (Picoides pubescens).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Piciformes; Picidae; Dryobates.
OX NCBI_TaxID=118200 {ECO:0000313|EMBL:KFV68986.1, ECO:0000313|Proteomes:UP000053875};
RN [1] {ECO:0000313|EMBL:KFV68986.1, ECO:0000313|Proteomes:UP000053875}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_N307 {ECO:0000313|EMBL:KFV68986.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May play a role in motor coordination and exploratory
CC behavior. {ECO:0000256|ARBA:ARBA00003652}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +
CC N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43140, Rhea:RHEA-COMP:10359, Rhea:RHEA-COMP:10360,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.216;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU00958};
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Trm1 family. {ECO:0000256|PROSITE-ProRule:PRU00958}.
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DR EMBL; KL216285; KFV68986.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A093IQJ1; -.
DR STRING; 118200.A0A093IQJ1; -.
DR Proteomes; UP000053875; Unassembled WGS sequence.
DR GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0160104; F:tRNA (guanine(26)-N2)-dimethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0007610; P:behavior; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.56.70; N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR042296; tRNA_met_Trm1_C.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR10631; N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10631:SF1; TRMT1-LIKE PROTEIN; 1.
DR Pfam; PF02005; TRM; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 3: Inferred from homology;
KW Behavior {ECO:0000256|ARBA:ARBA00022610};
KW Isopeptide bond {ECO:0000256|ARBA:ARBA00022499};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; Reference proteome {ECO:0000313|Proteomes:UP000053875};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU00958};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; tRNA processing {ECO:0000256|PROSITE-ProRule:PRU00958};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 108..128
FT /note="C2H2-type"
FT /evidence="ECO:0000259|PROSITE:PS00028"
FT REGION 521..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 522..547
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KFV68986.1"
FT NON_TER 649
FT /evidence="ECO:0000313|EMBL:KFV68986.1"
SQ SEQUENCE 649 AA; 73518 MW; 8E1B4F081E5063BD CRC64;
EKHISIQRDF AELEKLANLK EGEFTTAAPQ NTDLQVTDEK KPCPLCSEEK YKACYSHKLH
RHLLNFHWKV SVEFEGYRMC ICYLPCHPVK PNLIGDQTFS KMGAHYHCII CSATITRRTD
MISHINRHVN KGETESRFIT VPAPKNSHQV LKESATDVRV LPNYSTPQKT DSYFNPKMKL
NRQLIFSALA VLAKERTPIE CLDAFGATGI MGLQWAKHLR SSVRVTINDC NENSVAMIQE
NCHLNKMKVK LNTKEDDNDE AMRDGEDNTD TIEVTKMDAN VIMHLRSFDF IHLDPFGTTV
NYLDSAFRNV RNLGIVSLTS TDISSLYSKA QHVALRHYGC NIVRTEYYKE LAARIVIAAV
ARAAARCNKG IEVLLAVALE HFVLVVVRVL RGPSSADDSA QKIRYLIHCQ WCEERTFQKE
GNMLEENPYQ QLPCDCHGSM PGKTAVVLGP LWSGALFNAG FLRRMYFESA QYGLDETQSL
LKTLVCEAEC TTLKHYSIHT SCEETKQEEC GVYIKTPNTS PESLVHRKRK SDEVTRNTNK
RQKAENSAEH PAFYYNIHRH SIKGMNMPKL NRFLNYLSEA GYRVSRTHFD PMGVRTNAPL
AQFKTVLLKY STPTYGGGQA EDAEVLEEDR SGTAAIVFTK DCPPHCAAD
//
