ID A0A093X8T5_9PEZI Unreviewed; 4075 AA.
AC A0A093X8T5;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=V490_07231 {ECO:0000313|EMBL:KFX89105.1};
OS Pseudogymnoascus sp. VKM F-3557.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1437433 {ECO:0000313|EMBL:KFX89105.1, ECO:0000313|Proteomes:UP000029320};
RN [1] {ECO:0000313|EMBL:KFX89105.1, ECO:0000313|Proteomes:UP000029320}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-3557 {ECO:0000313|EMBL:KFX89105.1,
RC ECO:0000313|Proteomes:UP000029320};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFX89105.1}.
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DR EMBL; JPJS01002166; KFX89105.1; -; Genomic_DNA.
DR STRING; 1437433.A0A093X8T5; -.
DR HOGENOM; CLU_000215_0_1_1; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000029320; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000029320};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 3739..4075
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 260..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..370
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 755..807
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 953..977
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1553..1617
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1652..1681
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2018..2065
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2120..2147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2386..2596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2610..2648
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2696..2738
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2885..2977
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3049..3075
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3228..3247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3358..3436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..284
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 348..362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 954..968
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1575..1590
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1659..1680
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2041..2065
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2432..2480
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2492..2529
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2541..2566
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2611..2644
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2885..2929
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3049..3065
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3358..3380
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3418..3436
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 4042
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4075 AA; 449977 MW; F5419C96BA9F6A9F CRC64;
MVKILKTIQP KHKETLSPYI QSFVQNASTA PWHALPQVLA DFPTRWPFPR GDLYHWIPLL
NRFDSDLETF CTTYALNNGP QVKDFGCELL EKGGATPEQI SAAGFGPEGD REVIERILHF
SRILLENCGN RSIYSSSTHL SDLLNSTSLS LLAATLQIGT QLAQRYQATF RRIASSHRQT
NATLLANHYN IDLDRVQQLS QPFSRTVTTT ETTAPTVPNT PVTPNAKGKD KAYFSIPVAG
SKTTTSTSYA NDLVSLVRSG TLPDPSPKGK KAASTSSQTS AEVDWNEWGD VRITYYETQP
RGEEASQPTA PTASAAPVTP TPVRRTSMLG NQHGNRTSRA PAPEEAPATP SRSSTLPAAA
PTEEKATSSL KVVDIPADKV SEHSVQELVQ EYLPSVPDAS KYTLLTKLRV AKALSTSLDT
RRQMLAVRLL AVTNLAYIYP EPSFLEKVLK QDSDEPRRLQ LIYQLAELIH PPAQGDLGIP
RSLQTIAVNA LEGLAQHQHK YQDVCTALNT NVNHGVLLYI VRKAVAEMAT DEPGDKHTEN
DEWHSALFAL LSHVATLPRA GNELVTAGLV PILVEVLTLR TSVAEGSYPR VLNFLDAIVF
NVKDAFQVLA NADGLDAISN LIVHEVQSST EVASKGEGMP ADYRSPSIDY EIPYYQQQSI
KWLFKFIHHM MNQAGGYGGN FDRLLRNLID SPQLLGSLRE IISHAKIFGS SVWTNAVSIL
NDFINNEPTS FAIIAEAGLS KAVLEAVLGH EITVAAPKDG EPQAEATNED GSPARTDDDS
VYSPAVHDQT PHPPTQQMLE TPRPGPLARG IMPSSEAIII VPEAFGAICL NNAGMKMFLE
SNALQSFFEI FESPAHIKVL DNDANNLATS LGATFDELVR HHPALKTSIL NAVLDMVARV
SHLCKTRTKK EKLGAKLLVT NANGDVVVAD DGLLSSDEDK GKGKAVDTDA DVEMTGVSTE
TTEQSKVSKP LAPAEDDREN NVTPYIAAVA NFLSALFNNA GIRNQFMENG GVEPILDLAE
LPVLPSSPSD ERSHRNMHTV IALLAEQKAH ILVPSLIKRA QAAADTLLPF AQHQGPEAYF
AKYIDDDKVT GDNSYNLARG TSFVEAMVTT QNLCMALRQC ISSPIYSRNQ TSSFSSMNLA
DYYVRLVKSL GGILAASIKE DFKLMKLAPR HWRSAGRQSV QLFMNDAPEP LIPTIDSTEE
ALHSLLPEAQ GTAPMATLVT DSSGAENPIH KPATTKTLST EEKVSPEFKN FQNLHYLLGK
MPISLSIFFQ VIGKGLVPKR TPDSFQRQSN IAIANAIVEA VLSQLTSVSD ESSSETYTFW
VLMFRTVKDI LVNNARTAER PQECITLVVQ AFIKAGGFNT INHMLGLFDA EVRNNADKTV
NFNDVDLLLR NDLATLGIKH ILELYGPLVT GKNVGESFQS TTLQSRTDRD RNSPNYFVAG
QFVVEVRMAI LPAVQKLWES DIVEKGSKII PNKLIDIIRN VMETDFEEGA FKNADKALVP
YKQGKKKWRV SRENLAKLQA SEYEEDLAIE ALFRCNNSYS SAVEYCKAQL SERSGGRNRF
GNDDYDPGNG ANDTPGHETR PSTGSITPDQ GAAERDNGAD EAANDGVPPP VLTQSPEEFA
PNQANSLQQM LLDNMNRTLE NAEAGNLFAA ISGGGEQTNL PERVPNQQPS PSSADKTLES
QPEVATVERL NAERSKIRSN LIERCLEVIN AHGEFTFEVA DLITTVIEKS PEQAEMRATM
GGTLVVALTS FLSDDVRQVG KKIASYAHLL ALMLQDPQFY KASLEELKSN LGNLLEFVKL
APDHKSEESS PWVAHILLIV EILLSDDAEP RETEWKIPTS ETSAIDQPVL KAPELVVSTE
DRATLFEAIL DMLPRIGKDE SLALAVMRIL VILTRTRHIA VAMGEKKNIQ RLFVMAKQLA
GAATPRIQSP LMLILRHIVE DDETIKQIMR SQIKHFLDPG SSRSSRNPDI TLYLRAMSHL
VVRQPELFVE VNNEMVKLVK WTTTAPDHPG RQQIELQDKY KRTRRGKPEE AGLPTLKPTE
ELSMEDVKPS TETADGEAAE GSKVEHKVPI VENPDGVIYF LLCELLNYRD VEDKEAAPAP
TEDKTKASTT ETPAAAITGA EASAPAPGAA EPRPASPSKL ETKKPKPEFK AEEHPIYIYR
CFLLQCLTEL LASYNRTKVE FINFKRNAPT HAATPSKPRS NVVNYLLFDL IPSGSLDHAE
TLAMRKKVST SSWADSTITA LLSKTGEQIL DKERDNTDSD DEPDLLFVRK FVLENIIKAY
REASTSTEPL DIKYSRMLSL ADLMSHIMSG KDNIGGGSDP NVSIRSQQQL KRIMFEKGYI
GALTASIADI DLNFPGAKRA VKHILRPLKA LTQTAIELSE VGKVALTPGQ HDEDEIASAS
SVSDVDDDRE ETPDLFRNST LGMFEPDRDH ESSSEGEDDD EEMYDEDGYE EEMEGYEDGP
IEDDEDNISD EDEDLEGMGE IEGLDGDHGM DVEVIMDDDD EDDESGSSDD DDEDEDDDMD
EDDEDDRVQI IDNEGNPHPL AELDDEEDWE SEDEDEEEDY EGMAADEEEE AMHAGGHGPL
GHLVHAFGGD PHHHGEIDNL LQRIEDERIG VDPDEYLEED HEEDDEVDDE DEEMDDDEDM
YEEYQPEPGM PFTFEWNDEQ EPPMIISHRG HRAHGAPNPY MFGAHRDPLG VRDYYRGSYR
SHRPGPTPRD NNDGTNPLLQ RGDRAREPGT PGSTPFMGRE TLFNIVGRGP FGAEGGGPAA
ILNDLFTQLP FPAGALPSAS RNGGALHFHV TGGPGGADAR EFQAMFGGMR PGMARGHDRG
PGAAEPGAAT TFTTQLTLVR WSEESRLLFG PRHPEMALRV LMGLMAVIVP GALEAEKKAL
QEKKEAEEKK KKEDEEKAAQ AAKEAEEKAA KEKKEAEERE AEERARAEEA AAAAAAAPPA
EEHEEAAEAQ PMEGVESGEA AAAEGEGAPA EDRPRVTIPF RDGTLDITEL GIDLDYLDAL
PEELREEVIT GAIAQRRSDA AATGAPPSEI DQEFLNALPD EIRAEIIQQE RQDRRRRERD
EARRQAAANG APVPVPQEMD AASILATLPA DLRAQVLAEQ DEDVLAQLPP EYIAQARASM
GGGHHPLGRL TRVIDGARPA RPPGGEIVKP VRRSIVQMLD KPGVATLLRL MFIFQQDSLR
NTLYQVLQNI SENRHNRGEV ISTILHILQD GSADMTAVER SFAHLSVRAK QPKDKDPKTP
LSLSRKNTGT GLGALTTVPN TDISPLMVVQ QCLGALIYLC KVNLHVPSFF LTEHESAVSG
LKRNASRKGK GKDIKALKFP LNSLLVLLDR KLIMESSPVM ESLSDLLSRI TAPLQTFERK
QKEAEEAAKK AAEKPAEAEG EAAATEQQPE TMDAEPTAPA AEEAQPTEAG ASTAEAPKAD
GEKADDRAKE SEKKKVRPFV PPVVPDANLK LVINIFVARE CSAKTFRETL STIKNLSCLP
GAKAVFGKEL ICKAQELGEV ILLDLEELFP QIQKASNSTE LQGVALTKFS PSGSDQNKLL
RVLTALDHLF DPKNARKDSA AETEAGSETA DTEKQDLLAT LYENSTFGPM WEKLSACLSA
IRQRDHMLNV ATILLPLIES LMVVCKNTTL KDQPLRGKDN MLTSPVPESR MESLFFTFTE
EHRKILNDLV RNTPKLMSGT FSLLVKNPKV LEFDNKRNYF NRSIHNRNNP TRQSYPSLQL
SVRRDQVFHD SFKSLYFKSG DEMKFGKLSI RFHGEEGVDA GGVTREWFQV LSRQMFDPGY
ALFIPVSSDR TTFHPNLTSS INPEHLMFFK FIGRVIGKAL YEGRVLDCHF SRAVYKRILG
KAVSVKDMES LDPEYYKSVV WMLENDITDI ISESFSVDND KFGVVETVDL IPNGRNIPVT
EENKHEYVRL MVEFKLTGSV QEQLDNFLKG FHEIIPAELV AIFNEQELEL LISGLPEIEV
DDWKANTEYH NYTAASPQIQ WFWRALRSFD KEERAKMLQF VTGTSKVPLN GFKELEGMNG
FSRFNIHRDY GSKDRLPSSH TCFNQLDLPE YESYEMLRQQ VLTAITTGSE YFGFA
//
