ID A0A093XIS5_9PEZI Unreviewed; 738 AA.
AC A0A093XIS5;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=Thioesterase domain-containing protein {ECO:0008006|Google:ProtNLM};
GN ORFNames=V490_08967 {ECO:0000313|EMBL:KFX86659.1};
OS Pseudogymnoascus sp. VKM F-3557.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1437433 {ECO:0000313|EMBL:KFX86659.1, ECO:0000313|Proteomes:UP000029320};
RN [1] {ECO:0000313|EMBL:KFX86659.1, ECO:0000313|Proteomes:UP000029320}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-3557 {ECO:0000313|EMBL:KFX86659.1,
RC ECO:0000313|Proteomes:UP000029320};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFX86659.1}.
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DR EMBL; JPJS01002602; KFX86659.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A093XIS5; -.
DR STRING; 1437433.A0A093XIS5; -.
DR HOGENOM; CLU_016508_0_1_1; -.
DR Proteomes; UP000029320; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18617; GH43_XynB-like; 1.
DR CDD; cd03443; PaaI_thioesterase; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR Gene3D; 3.10.129.10; Hotdog Thioesterase; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR006683; Thioestr_dom.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF03061; 4HBT; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR SUPFAM; SSF54637; Thioesterase/thiol ester dehydrase-isomerase; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000029320}.
FT DOMAIN 349..517
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT DOMAIN 607..686
FT /note="Thioesterase"
FT /evidence="ECO:0000259|Pfam:PF03061"
FT ACT_SITE 16
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 199
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 145
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 738 AA; 82197 MW; 8A816BD00EA267D2 CRC64;
MSSTDINPII PGFAPDPSVV LVDGTFFLVN SSFHMFPGLP IYASKDLTSW KHIGNAINRQ
SQLKLTVSRT ILCPPHENGD VMLSTGGLYA PTIRYQNGTF YIVCTNVIHD DSSDDSKTEN
FVVSSSNIWS NEWSDAIYFD FDGIDPSIFF DDNGRAYIQG SAGPGPMTKI HLFEVDLKTG
KKLSEEKKIW DGTGGIYPEG PHIYKKDGLF YLVISEGGTH DDHMITVARS KDIWGPYEQF
EKNPILTARG TNEYIQYTGH SDMFQDSQGQ WWGVCLGVRK DESRFIMGRE TFLTTGSWPA
GEWPSLDLVK RNPKFPGGKE LVRADGTLPL TAEPTMDYLY IRDAVLGDHQ FSNDGKTVTL
TASQADMSQW EKPVTFVGKR QRLLEGNATV TMHKPTISSD AHLKAGIAYY KDEHRYIKLF
YDFSTSEMVF EVVNNAQKIS RTVRHDVQLE TVVALRIEYT ERSLQLSYRN GENSAPWVSF
DGLDTLEMTG PDFVGPVIGV FAISTTQNTH VQFDDLEVLF NGLSGKVSRR VYIIKNRGAM
AAVSEAERAK AIMSIQSVFD RFHLISADAP VKFFDDQVMR DAKVVDGSPD GKASFEIRIT
PYYGNINGVL HGGAAGVIFD MLTSPALAPL SRPGFWDFLG GVTRVLNVSF LRGVPIGTVV
RVNSKVMQVG RTMAMIQSEM TSLDGKIIYC TAEHHKVSVA TPAHHLSEKY EVEWDRVMKA
EGKKPNGLWT PKKVKGKL
//
