ID A0A093Y8C9_9PEZI Unreviewed; 1539 AA.
AC A0A093Y8C9;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN ORFNames=O988_02817 {ECO:0000313|EMBL:KFY01276.1};
OS Pseudogymnoascus sp. VKM F-3808.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1391699 {ECO:0000313|EMBL:KFY01276.1, ECO:0000313|Proteomes:UP000029329};
RN [1] {ECO:0000313|EMBL:KFY01276.1, ECO:0000313|Proteomes:UP000029329}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-3808 {ECO:0000313|EMBL:KFY01276.1,
RC ECO:0000313|Proteomes:UP000029329};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFY01276.1}.
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DR EMBL; JPJR01000449; KFY01276.1; -; Genomic_DNA.
DR STRING; 1391699.A0A093Y8C9; -.
DR HOGENOM; CLU_001517_2_1_1; -.
DR Proteomes; UP000029329; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Reference proteome {ECO:0000313|Proteomes:UP000029329};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 42..137
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 140..577
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 732..976
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1054..1522
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
FT REGION 1410..1437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1419..1434
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1539 AA; 172780 MW; D8F38FCD1BDEFCAF CRC64;
MAPNTSNAAK EVYLLPLLDD GSPDVPGGYI YLEPTSAEPI IVRFAIEGTS SICRHGSLWV
NINAPGEPFK RDAFREFKLE PDFNRTLEID IPIHSSGAFA FYTTYSPLPD LDDAGSKPSE
PTKTPLYYID VAPRLSLGEK QLPMAALSLI SVLSKFMGKY PTDWQKHLRG ISERGYNMIH
FTPLQVRGHS NSPYSIADQL GWDPECFPGG EKDIAQLVNV MEKEHGLLGL TDVVWNHTAD
NSTWLQEHPE VGYNMSTAPW LESALQLDTE LLKFGRELEK LGLPTDIKTV EDLLLIMDGI
KTKVIGGLKL WEYYAIDVKR DVDAAVDAWV SGNVKAPAEG FGSKDASPKE HAKFIVDKAM
LGTDRLGERY RRKVDPAIAA ALLTEWHGKF NGNASNPADS EEVRSHTSKI FDEVNLPFYK
EYDEDIAEVL EQIFNRVKYV RLDDHGPKLG PINDKNPLIE SYFTRLPQNA TTKKHKQEDL
ALVNNGWIWA ANALVDNAGP NSRAYLRREV IVWGDCVKLK YGDGPQDSPY LWERMANYTR
LMAKYFTGLR IDNAHSTPLH VAEYLLDEAR RVRPDLFVVA ELFTGSEEMD YVFVKRLGIN
GLIREAMQAW NTGELSRLVH RHGGRPIGSF EVDEISGNDA KESTEIVRKI KQTPVRALFM
DCTHDNEVPA QKRDARDTLP NAALVYMCAS ATGSVFGYDE IYPKIIDLVH ETRLYTSHSS
EKPVDIKDGD GGIGGVRKLL NQIHILMGLD GYEETHIHHD DQYVTVHRVH PEFRKGYFLI
AHTAFPGYKN GNGPFSPVHL TGTKANHLGS WMLEVDDSEE ARKAASEDKQ YLKGLPSKVT
SVPGIKMESK DDETVITMGD KFPPGSIALF ETWIPAAEHA SGLDTFVTSG AKEAFSKVDL
VDLNFIMYRC EGEELDSSDG KDGVYDIPSH GKLVYAGLEG WWSVLKKVIN ENDLAHPLAQ
HLRSGQWALD YTVGRLQRKS KEEAFERLQA PALWLQERFD AIRKLPSFLL PRYFGLIIKT
AYSAGFDRGV ELMSENVQKG QWFMKSLAMV SVQQTGFVKS ASLYPKRAVP SLAAGLPHFA
VEWARCWGRD VFISARGLLL GTGRYAEARE HIIAFASVVK HGMIPNLLSS GNLPRYNSRD
SVWFFLQTIQ DYTKIVPNGH DLLQEKISRR FLPYDDTYFE SDDPRAYSKT STLEDIIQEI
FQRHASGMSF REANAGPKLD MQMKPEGFQI DIKVDWNTGI IFGGSQDNCG TWMDKMGESE
KAGTRGVPGT PRDGAAVEIT GLSYSALKWV SQLNKNGKFK YSGVNTSDSS IKEISFADWA
SKIRDNFERC YYVPASSKED ANYDVNPAII NRRGIYKDLY KSGKEYEDYQ LRPNFPIAMT
VSPDLFDDKH ALGALFIADK ALRGPTGMAT LDPSDLNYRP DYHNSEDSTD KATSKGRNYH
QGPEWLWPTG FFLRALLAFD LKRRDTPEGR TEAFQQVTRR LADSKKAIVE SEWAGLTELT
NKNGSFCADS SPTQAWSAGC FIDLYHDAAQ YDISQLQEK
//
