ID A0A094CZK8_9PEZI Unreviewed; 860 AA.
AC A0A094CZK8;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=V496_05615 {ECO:0000313|EMBL:KFY59616.1};
OS Pseudogymnoascus sp. VKM F-4515 (FW-2607).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1420909 {ECO:0000313|EMBL:KFY59616.1, ECO:0000313|Proteomes:UP000029302};
RN [1] {ECO:0000313|EMBL:KFY59616.1, ECO:0000313|Proteomes:UP000029302}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-4515 (FW-2607) {ECO:0000313|Proteomes:UP000029302};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFY59616.1}.
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DR EMBL; JPJY01001028; KFY59616.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A094CZK8; -.
DR STRING; 1420909.A0A094CZK8; -.
DR HOGENOM; CLU_005015_1_1_1; -.
DR OrthoDB; 2504097at2759; -.
DR Proteomes; UP000029302; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000029302};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 199..303
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 692..784
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 787..845
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 860 AA; 97863 MW; AB9D3F553E40D145 CRC64;
MTLHTTIPIN EDWEFKQVDS KKSKWLPVAQ FPTNVHLDLI ANNIIADPFM GKNELDVQWI
GEAVWAYKTT FETPAASAEE GSAAKAVLAF DGLDTYATVV LNGTEILKTE SMFIRERVDV
TTYLNKDGAN ELEITFESAF LKGKQIVDKY PEHKWGCWNG DASRLAVRKA QYHYGWDWGP
TMLTCGPWRP INLEIYESRI ADLYFRTDVE ESLKAAKIIA TADIEGSASL VKFDIALDGE
AVASEQAKVV DGRATATFNI TDPALWYPIR YGKQPLYTIT ATLVATRSEA YDIHVESKRF
GLRRAELIQR PLKDEPGESF FFQINNVPIF CGGSDWIPAD NFVPRITPEK YYDWVKLVAD
GNQVMIRVWA GGIFEEAPFY DACDELGILV WQDFLFGCGN YPAFPEFLDL VKREADDNVK
ILRHHPSIVI WAGNNEDYQY QESENLTYDF ENKDEESWLK TDFPARYIYE KILSDTCREL
VPDVPYHPGS PWGKGRDTHD STVGDIHQWN VWHGSQEKYQ NFDKLGGRFV SEFGMEAFPN
IKTIEAFLPL GKDDPDRYPQ SSTVDFHNKA DGHERRIALY LVENMQYSPN PIEQYIYSTQ
LMQGECLASA YRLWKRQWKG PGREYCAGAL VWQINDCWPV TSWAIVDYYL RPKHAYFTVK
REMAPLSCGI TRTLTTTPRD KHTRVYIDTK HTIEIWGSNL TLRDMVVDVV VRAFDVETGR
ESYNKTVKTA FALPQNQSTE IIAFDVPVKH RDAGEEARVV VAAYLFEDGV QVARYVNWPE
PLKYNHLTKP QCLTATLTAD ARTVEISAEV PVKGLALECD DEDVKFADNL VDIVPGEVVS
IGVVGARKNT VIGTRYLGMI
//
