ID A0A094DA39_9PEZI Unreviewed; 1081 AA.
AC A0A094DA39;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=alpha-amylase {ECO:0000256|ARBA:ARBA00012595};
DE EC=3.2.1.1 {ECO:0000256|ARBA:ARBA00012595};
GN ORFNames=V497_06396 {ECO:0000313|EMBL:KFY56325.1};
OS Pseudogymnoascus sp. VKM F-4516 (FW-969).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1420910 {ECO:0000313|EMBL:KFY56325.1, ECO:0000313|Proteomes:UP000029268};
RN [1] {ECO:0000313|EMBL:KFY56325.1, ECO:0000313|Proteomes:UP000029268}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-4516 (FW-969) {ECO:0000313|Proteomes:UP000029268};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC polysaccharides containing three or more (1->4)-alpha-linked D-
CC glucose units.; EC=3.2.1.1; Evidence={ECO:0000256|ARBA:ARBA00000548};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFY56325.1}.
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DR EMBL; JPJZ01001103; KFY56325.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A094DA39; -.
DR STRING; 1420910.A0A094DA39; -.
DR HOGENOM; CLU_009402_0_0_1; -.
DR OrthoDB; 2165012at2759; -.
DR Proteomes; UP000029268; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd11319; AmyAc_euk_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 2.
DR InterPro; IPR015340; A_amylase_C_dom.
DR InterPro; IPR006046; Alpha_amylase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR23501; MAJOR FACILITATOR SUPERFAMILY; 1.
DR PANTHER; PTHR23501:SF107; TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G04730)-RELATED; 1.
DR Pfam; PF09260; A_amylase_dom_C; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR00110; ALPHAAMYLASE.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000029268};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 86..103
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 123..142
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 154..171
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 177..199
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 211..228
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 240..263
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 288..312
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 324..346
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 366..383
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 403..426
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 433..452
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 458..479
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 491..519
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 626..978
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 29..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1081 AA; 116977 MW; B1BA51FBB9874284 CRC64;
MSDSCIVRMK LAIFSRVMGE RAVAPTVLSP DEMNCETTAS SGKAEKELVP TEEGEPSSDE
EAPSETAAEG IKKVEAIAIV WSRSELYAAY ACVFLVYFVN SLQQQIISNL LPYVMSDFAS
HSLIPVTGIV SQILGGVLKL PIAQLINIWG RPQGLIVMTT LCTIGLILMA VCNNVQTYAA
AQCFYWVGYN GIGFVLDVFI ADTSNLRNRA LAFAFASTPY IVTTWAGPKA AESFYAHSGW
RWAFGCFSIL TPIVILPLLY ILFSNQRKAA RLGFIQKRES GRTVKESLWY YFIEFDVVGI
LLISAGFALF LLPFALAGSQ TDKWAAASTI IMLVLGVALL AAFGLYEKYL APRSFIPFHL
VTDRTVLGSC GLSAVSFASF YLWDNYYLSY LQVVHQLSIT TAGYVFNIFS IGCSFWAVVV
AAAIHYTGRI KPLALYFGAP LNLLGVGLMI HFRQPSHSIG YVVMCQIFIA FAGGILVICE
QMAAMAAVKH ADVAAILAVL GLSAAVGGAI GSSMAGAIWT NTLPDALTAA LPEAEKARAG
EIYGSLKEQL AFKWGSEARM GIVQAYGIAQ RRMTIASTSV IVLMFIKVMF FNRPALVARY
LAVAAALFTS TSAKTAAEWR ESSIYQLLTD RFATTEGSAP DCSIRSYCGG TWKGIENKLD
YIQGMGFDAI WISPVIHNIE DNTQYGNAFH GYWGDDPFTL NPHFGTGDDL KSLSDALHGR
GMSLMIDVVI NHLAANQPST SVDYSVFPAP FNSASAFHTP CAIDYTNQGS IENCWLVTDT
PSLPDIDSEN GDVYTAMVNS VADIVKTYGV DGIRLDTARH VPKEFLTQFQ EAVGVFVTGE
VLDGNPDFVA GYQGPLSSTL NYPLWYPLVD SFMGGDFSAL ATTMGTVGSV FTDVNALANF
LDNHDQPRFA SREGNDVVRD RNAATFLMFT SGIPVVYYGF EHRFGGASDP DNREALWGSG
YNVDAELYKH IALLHQIRDL ASGIGEKAAY FSTSAEVIGT STQYMAIKRG AVVVVVSNVG
AQGATDGFNV SGSQFDSGDQ IVDLFSCTTA TVGDGGAFDS TANNGEARIW IRSRDKGSFC
P
//
