ID A0A094DJ29_9PEZI Unreviewed; 845 AA.
AC A0A094DJ29;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 03-MAY-2023, entry version 27.
DE RecName: Full=Arabinogalactan endo-beta-1,4-galactanase {ECO:0000256|ARBA:ARBA00012556, ECO:0000256|RuleBase:RU361192};
DE EC=3.2.1.89 {ECO:0000256|ARBA:ARBA00012556, ECO:0000256|RuleBase:RU361192};
GN ORFNames=V497_00872 {ECO:0000313|EMBL:KFY66416.1};
OS Pseudogymnoascus sp. VKM F-4516 (FW-969).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1420910 {ECO:0000313|EMBL:KFY66416.1, ECO:0000313|Proteomes:UP000029268};
RN [1] {ECO:0000313|EMBL:KFY66416.1, ECO:0000313|Proteomes:UP000029268}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-4516 (FW-969) {ECO:0000313|Proteomes:UP000029268};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic
CC linkages in type I arabinogalactans.; EC=3.2.1.89;
CC Evidence={ECO:0000256|ARBA:ARBA00001695,
CC ECO:0000256|RuleBase:RU361192};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the TDE1 family.
CC {ECO:0000256|ARBA:ARBA00006665}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 53 family.
CC {ECO:0000256|ARBA:ARBA00010687, ECO:0000256|RuleBase:RU361192}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFY66416.1}.
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DR EMBL; JPJZ01000114; KFY66416.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A094DJ29; -.
DR STRING; 1420910.A0A094DJ29; -.
DR HOGENOM; CLU_337110_0_0_1; -.
DR OrthoDB; 53161at2759; -.
DR Proteomes; UP000029268; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031218; F:arabinogalactan endo-1,4-beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0015926; F:glucosidase activity; IEA:InterPro.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR011683; Glyco_hydro_53.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR005016; TDE1/TMS.
DR PANTHER; PTHR34983; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1.
DR PANTHER; PTHR34983:SF1; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1.
DR Pfam; PF07745; Glyco_hydro_53; 1.
DR Pfam; PF03348; Serinc; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361192};
KW Hydrolase {ECO:0000256|RuleBase:RU361192};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000029268};
KW Signal {ECO:0000256|RuleBase:RU361192};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|RuleBase:RU361192"
FT CHAIN 21..845
FT /note="Arabinogalactan endo-beta-1,4-galactanase"
FT /evidence="ECO:0000256|RuleBase:RU361192"
FT /id="PRO_5005107446"
FT TRANSMEM 12..37
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 49..71
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 91..116
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 137..155
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 161..182
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 203..227
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 233..254
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 306..324
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 377..398
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 845 AA; 91641 MW; 3965544B60E09A37 CRC64;
MGALLSIPLL AVPSIGTVLG FAASCCGAAT CSAICSMCGK CGNSVATRIA YALILMVNSI
FAWIMLTPWA INKLQHLTLD YMTISCPEGA CYGWVAVHRI NFALGILHLL LALLLLGVRS
SKDQRAGIQN GFWGPKIIAW LALIVLSFLI PDGFFMVWGN YIAFAGAMLF LLLGLILLVD
LAHTWAEYCL SQIEENDSRA WRGILIGSTL GMYAISITMT VVQYVFFAGG GCSMNQAAIT
INLILLFIVS AISVHPGIQD YNPKAGLAQS AMVAIYCTYL TMSAVSMEPD DKHCNPLVRG
GQATRTTTVV IGAIVTMLTV AYTTTRAATQ GVALGGSTQS IRLPDDEHGL ITTQPDSRRE
MRAAALRQAV AEGSLPADAL LDDDSDDESN VGQTGKDDER SATQYNYSLF HIIFFLATAW
VATLLTMNFE EDSSEDGLDF VPVGRTYWAS WVKIAPSTGR RGCWHPLTAF NPTVQIDHKE
GIKSIFLELT NMLLLRSLFV AIPFVGFSTA ALTYKGADWS SVPVEEAAGK SYKNTAGTTQ
PFETILKASG ANTVRQRIWV NPSDGNYNLD YNIKLAKRAK AAGLKIYLDL HYADSWADPG
KQPTPSAWAS QNIDDLSNTV YEYTLNIANT FASNGIDVAL MSIGNEITAG LLWPLGKIAN
SGGAYNTARL LHSAAWGIKD SNWGTKPLIM IHLDNGWNWD TQKWWYDLIL SQGPLVASDY
DVQGVSYYPF YNSAATLASL KTSITNMKST YGKQVQVVET DWPTYCPNPA YAFPSDIKSI
PFSPEGQTTF MKNLASTISA AGGNGLFYWE PAWIDNANLG SSCGYNLMVD DTGKAMSSLA
VFASI
//
