ID A0A094FTK1_9PEZI Unreviewed; 1462 AA.
AC A0A094FTK1;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=V500_04346 {ECO:0000313|EMBL:KFY92048.1};
OS Pseudogymnoascus sp. VKM F-4518 (FW-2643).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1420913 {ECO:0000313|EMBL:KFY92048.1, ECO:0000313|Proteomes:UP000029284};
RN [1] {ECO:0000313|EMBL:KFY92048.1, ECO:0000313|Proteomes:UP000029284}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-4518 (FW-2643) {ECO:0000313|Proteomes:UP000029284};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFY92048.1}.
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DR EMBL; JPKC01001430; KFY92048.1; -; Genomic_DNA.
DR STRING; 1420913.A0A094FTK1; -.
DR HOGENOM; CLU_001493_6_0_1; -.
DR Proteomes; UP000029284; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR021858; Fun_TF.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR InterPro; IPR001138; Zn2Cys6_DnaBD.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF11951; Fungal_trans_2; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000029284}.
FT DOMAIN 38..669
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 708..861
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 992..1023
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..25
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 996..1018
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1462 AA; 162347 MW; 32573716A92CF222 CRC64;
MRVPSAKSWS STIRLPKSTF PPRTTPADQA KYLQRCTDDL YAWQAKVRAQ QKPFVLHDGP
PYANGSLHAG HAVNKILKDL ICRTELQQGR KVQFVPGWDC HGLPIEIKAL EQVRAKSEDG
EVNLDPIGIR RAARNLARKT VKEQKKGFRE WGIMADWENP WVTMDKDFEL KQLEVFLEMT
RQGLIFRRYK PVYWSPSSKS ALAEAELEYN ESHESITAYI KFQVVAVPEA LKAQGVDTGR
LHAVIWTTTP WTLPANKAIA VSPDLEYTVV EVGSEQLLIA KSRLEHVAAK CAFPESFKVI
VESIKGSELE GLTYVNALHG ENAPIQPLIF ADYVSDASGS GLVHTAPGHG MEDYDVCTKL
GIEASAPVND AGCFTDEALP HNPSALAGKS VLGGGNEAVL ALLGFQVLGV HKHEHKYPYD
WRTKLPVIVR ATEQWFADVG SIKDKALKSL DNVKFIPENS RSRLESFIKG RSEWCISRQR
AWGVPIPALY DESGKAILTQ ESVSHIISVI QERGIDSWWT DAQDDVSWVS PNLRGQGTLK
RGTDTMDVWF DSGSSWTQTE GPADVYSEGT DQHRGWFQSS LLTHISASGL DSAPFKTLVS
HGFTIDEHGK KMSKSIGNVI SPAEIMNGTL LPPIKRKGKA TGAPAYDGLG ADALRLWVAS
SDYTHDIALR QPVFKAINST LLKYRTTLKM LLGSMRESSE KVQLTKLDTI ALYQLQQTQI
GFAEACRAYN FHKAISILNR WINTDLSSFY LEAIKDRLYC GNGGGVLYPI FMGLMHMLGP
ITPALVEESW EHRPQWMKDN TSSVHPLQRS TDELRLLTYK FPPSLPTDIS HLMAANAAIK
TAQEEARAEK LVGSSLQSSV VLTLPKESQD LFQNYADELQ EIFVVSSVEF GSGEDRVAEW
KFKAEFEVPG GKGTAWVLPP KEAKCPRCWK YVAPAEDELC GRLLSSVATT RCDEVTPHCT
QCTSTGRECP GPITGVIISD MTSNVRRRAR SKLANRSLGV SHSRSSGRNP SQAGQYEPVP
NETADDYQNL DVNIIYSDPP DEPTLSLEPV NNNASSIERL GADLDPFESL PVKLHFSTLK
LLEYCTNCMR NAFVALGPGD PMMDIWLPMI LSDQLLLQCT LYTSAIHMST IFGIDVMESM
DVVTHKTETL SLLTKRLGDP NHAVNDVSLT AVARYVGQVI LCGNVQETKL HMDGLERMLA
LTPDLEPSCQ VREMLFWTDL TAAALCHAAP RQLTKRPNIR LYGNPLLQAP RSTIGILCRG
FQKIIADHEV FRSITDILHD LRCLTAVAHY SDCKVHSEMP IFVSLCFGVD ERLGNLLAME
GSAMFESCRL AAMVFVEIVL LHNNTPNAVL LAAGMKRTLS RANMEVMLQE QPELLTWILY
MGNIVAFGTE MAPWFLRSFA QALSFQRLSI FETLEAGAGG NRLTSDERPY HRKTADVLVT
RISELAVTLI IMSDDNKAEP EK
//
