ID A0A095C0D5_9SPHN Unreviewed; 359 AA.
AC A0A095C0D5;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=cholesterol 7-desaturase {ECO:0000256|ARBA:ARBA00026095};
DE EC=1.14.19.21 {ECO:0000256|ARBA:ARBA00026095};
GN ORFNames=FG91_00544 {ECO:0000313|EMBL:KGB56751.1};
OS Sphingopyxis sp. LC81.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingopyxis.
OX NCBI_TaxID=1502850 {ECO:0000313|EMBL:KGB56751.1, ECO:0000313|Proteomes:UP000029574};
RN [1] {ECO:0000313|EMBL:KGB56751.1, ECO:0000313|Proteomes:UP000029574}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LC81 {ECO:0000313|EMBL:KGB56751.1,
RC ECO:0000313|Proteomes:UP000029574};
RA Gan H.M., Gan H.Y., Barton H.A., Savka M.A.;
RT "Genome sequence of acyl-homoserine lactone-producing cave bacterial
RT isolate.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol + H(+) + NADH + O2 = 7-dehydrocholesterol + 2 H2O
CC + NAD(+); Xref=Rhea:RHEA:51644, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16113, ChEBI:CHEBI:17759,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.19.21;
CC Evidence={ECO:0000256|ARBA:ARBA00029355};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51645;
CC Evidence={ECO:0000256|ARBA:ARBA00029355};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol + H(+) + NADPH + O2 = 7-dehydrocholesterol + 2 H2O
CC + NADP(+); Xref=Rhea:RHEA:45024, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16113,
CC ChEBI:CHEBI:17759, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.14.19.21; Evidence={ECO:0000256|ARBA:ARBA00029369};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45025;
CC Evidence={ECO:0000256|ARBA:ARBA00029369};
CC -!- PATHWAY: Hormone biosynthesis. {ECO:0000256|ARBA:ARBA00004972}.
CC -!- PATHWAY: Steroid hormone biosynthesis; dafachronic acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00025712}.
CC -!- SIMILARITY: Belongs to the cholesterol 7-desaturase family.
CC {ECO:0000256|ARBA:ARBA00025729}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGB56751.1}.
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DR EMBL; JNFD01000002; KGB56751.1; -; Genomic_DNA.
DR RefSeq; WP_037511542.1; NZ_JNFD01000002.1.
DR AlphaFoldDB; A0A095C0D5; -.
DR STRING; 1502850.FG91_00544; -.
DR PATRIC; fig|1502850.3.peg.545; -.
DR eggNOG; COG4638; Bacteria.
DR OrthoDB; 9800776at2; -.
DR Proteomes; UP000029574; Unassembled WGS sequence.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0044270; P:cellular nitrogen compound catabolic process; IEA:UniProt.
DR GO; GO:0008203; P:cholesterol metabolic process; IEA:InterPro.
DR GO; GO:1901565; P:organonitrogen compound catabolic process; IEA:UniProt.
DR Gene3D; 2.102.10.10; Rieske [2Fe-2S] iron-sulphur domain; 1.
DR InterPro; IPR045605; KshA-like_C.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR PANTHER; PTHR21266:SF60; CHOLESTEROL 7-DESATURASE; 1.
DR PANTHER; PTHR21266; IRON-SULFUR DOMAIN CONTAINING PROTEIN; 1.
DR Pfam; PF19298; KshA_C; 1.
DR Pfam; PF00355; Rieske; 1.
DR SUPFAM; SSF55961; Bet v1-like; 1.
DR SUPFAM; SSF50022; ISP domain; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|ARBA:ARBA00022714};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 18..131
FT /note="Rieske"
FT /evidence="ECO:0000259|PROSITE:PS51296"
SQ SEQUENCE 359 AA; 40563 MW; EEC0EDAB9BE55A1A CRC64;
MATTADYGLG DQMFPRGWFM IAAAAELEGT TPLAVRFFGR DRVLYRGESG QVYLVDAYCP
HMGAHLAKNE TSFVIRDNEH VQGESIRCPY HGWRFGPDGK CNQIPYSPQK IPAAAKLENW
PVIERAGCIW MWHDPEGLEP EYELPPFEEY YQPHWVKWEL DRLGELASHP VEILDNMADR
AHFEPVHGND AVDVFENDFD GHRVVQRLRA GHRTLQGDAP LETDTWYTGP GILQSRMSGQ
FPAIIMIAHT PVDDGVVKVW HALLVKVPNA EPTADDVAMA RAYQAAACAA FAQDFEIWSN
KRPCVNPMAV QGDGPIGKLR IWYRQFYNSR ARAEEFQKRV NGTHVTRGTL SAPFEQKVA
//
