ID A0A095TDV4_9GAMM Unreviewed; 1245 AA.
AC A0A095TDV4;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN Name=narZ {ECO:0000313|EMBL:KGD74897.1};
GN ORFNames=HA49_06305 {ECO:0000313|EMBL:KGD74897.1};
OS Tatumella morbirosei.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Tatumella.
OX NCBI_TaxID=642227 {ECO:0000313|EMBL:KGD74897.1, ECO:0000313|Proteomes:UP000029577};
RN [1] {ECO:0000313|EMBL:KGD74897.1, ECO:0000313|Proteomes:UP000029577}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 23360 {ECO:0000313|EMBL:KGD74897.1,
RC ECO:0000313|Proteomes:UP000029577};
RA Smits T.H., Palmer M., Venter S.N., Duffy B., Steenkamp E.T., Chan W.Y.,
RA Coutinho T.A., Coetzee M.P., De Maayer P.;
RT "The draft genome of the Tatumella morbirosei type strain, LMG23360T
RT isolated from pineapple rot.";
RL Submitted (DEC-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGD74897.1}.
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DR EMBL; JPKR02000004; KGD74897.1; -; Genomic_DNA.
DR RefSeq; WP_038017977.1; NZ_JPKR02000004.1.
DR AlphaFoldDB; A0A095TDV4; -.
DR STRING; 642227.HA49_06305; -.
DR eggNOG; COG5013; Bacteria.
DR OrthoDB; 9759518at2; -.
DR Proteomes; UP000029577; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR Gene3D; 4.10.1200.10; nitrate reductase tail; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR InterPro; IPR028189; Nitr_red_alph_N.
DR InterPro; IPR044906; Nitr_red_alph_N_sf.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR Pfam; PF14710; Nitr_red_alph_N; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000029577}.
FT DOMAIN 43..107
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1245 AA; 140656 MW; 8246E529D2B193B3 CRC64;
MSKLLDKLRY FKQKEGTFSQ EHGQMMNSNR DWEDGYRQRW QHDKIVRSTH GVNCTGSCSW
KIYVKNGLVT WETQQTDYPR TRADLPNHEP RGCPRGASYS WYIYSANRLK YPLVRKQLIR
LWREALQQHS DPVMAWDSIV NDEAKSASYK QARGRGGFVR SDWNELNQLI AAANIWTVKN
YGPDRVAGFS PIPAMSMVSY AAGTRYLSLM GGTCLSFYDW YCDLPPASPM TWGEQTDVPE
SADWYNSAYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTVA ITPDYAEVAK LSDHWLAPKQ
GTDAALAMAM GHVILKEFHI TNPSDYFLNY CRRYTDMPML VLLEHTSEGY YRPGRLLRAA
DLPENLGETN NPDWKTVAFN QQGQLVIPNG SAGFRWGQQG KWNLEARAAD QDQELRLTMA
DGYDDLCGVA FPYFGGQESP HFRHVKQSPI LIHTLPVRQI TLADGRQGQV VSVYDLTLAN
YGVERGFGDE QSAASYDELK ACTPAWAEQI CGVPRQQIIR IAREFADTAH KTHGRSMIIV
GAGMNHWYHM DMNYRGLMNM LIFCGCVGQT GGGWAHYVGQ EKLRPQTGWQ PLAFALDWAR
PPRHMNSTSF FYNHSGQWRY EKLTVKELLS PLANPDEFTG QLIDFNVRAE RMGWLPSSPQ
LNTNPLNIAQ HAKTAGLSPV DYTVEALQNG KLRFASEQPD SGSNHPRNLF VWRSNLLGSS
AKGHEYMLRY LLGIEHGIQG SETVAVPGMS PEEVDWQTKA REGKLDLLVT LDFRMSSTCL
FSDIVLPTAT WYEKDDMNTS DMHPFIHPLS AAVDPVWESR SDWDIYKGIA KTFSALCPGH
LGVESDVVLL PIQHDSPAEL AQPYEVKQWH KGECALQPGK NAPHIMVVER DYPALSERFG
SVGPLLEKLG NGGKGISWNT EKEVELLRQL NHRCDSGAGK GQPQLNTAID AAEMILALAP
ETNGQVAVKA WQALGKITGR DHTHLAMPKQ DEKIRFRDIQ AQPRKIISSP TWSGLEDEHV
SYNACYTNVH ELIPWRTLTG RQQLYQDHPW MRAFGESLVS YRPPVNTRSV EQLSHIPSNG
YPEKTLNFLT PHQKWGIHST YSENLLMLTL SRGGPIVWMS EQDAQEMGIR DNDWIEAFNS
NGALTARAVV SQRVPAGMTM MYHAQERITN IPGSEVTGMR GGIHNSVTRI SPKPTHMIGG
YVHQCYGFNY YGTVGSNRDE FIIVRKMKNI SWLDDEGRDQ IQEAE
//
