ID A0A097QZ50_HAFAL Unreviewed; 567 AA.
AC A0A097QZ50;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE SubName: Full=Hydrogenase 3 large subunit {ECO:0000313|EMBL:AIU71764.1};
GN Name=hycE {ECO:0000313|EMBL:AIU71764.1};
GN ORFNames=AT03_04685 {ECO:0000313|EMBL:AIU71764.1};
OS Hafnia alvei FB1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Hafniaceae; Hafnia.
OX NCBI_TaxID=1453496 {ECO:0000313|EMBL:AIU71764.1, ECO:0000313|Proteomes:UP000029986};
RN [1] {ECO:0000313|EMBL:AIU71764.1, ECO:0000313|Proteomes:UP000029986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FB1 {ECO:0000313|EMBL:AIU71764.1,
RC ECO:0000313|Proteomes:UP000029986};
RX PubMed=25075225; DOI=10.1186/1757-4749-6-29;
RA Tan J.Y., Yin W.F., Chan K.G.;
RT "Gene clusters of Hafnia alvei strain FB1 important in survival and
RT pathogenesis: a draft genome perspective.";
RL Gut Pathog. 6:29-29(2014).
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
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DR EMBL; CP009706; AIU71764.1; -; Genomic_DNA.
DR RefSeq; WP_025801577.1; NZ_CP009706.1.
DR AlphaFoldDB; A0A097QZ50; -.
DR GeneID; 56890515; -.
DR KEGG; hav:AT03_04685; -.
DR PATRIC; fig|1453496.5.peg.937; -.
DR eggNOG; COG3261; Bacteria.
DR eggNOG; COG3262; Bacteria.
DR HOGENOM; CLU_015134_3_1_6; -.
DR OrthoDB; 9801496at2; -.
DR Proteomes; UP000029986; Chromosome.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR Gene3D; 3.30.460.80; NADH:ubiquinone oxidoreductase, 30kDa subunit; 1.
DR InterPro; IPR001135; NADH_Q_OxRdtase_suD.
DR InterPro; IPR037232; NADH_quin_OxRdtase_su_C/D-like.
DR InterPro; IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
DR InterPro; IPR014029; NADH_UbQ_OxRdtase_49kDa_CS.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR43485:SF1; FORMATE HYDROGENLYASE SUBUNIT 5-RELATED; 1.
DR PANTHER; PTHR43485; HYDROGENASE-4 COMPONENT G; 1.
DR Pfam; PF00329; Complex1_30kDa; 1.
DR Pfam; PF00346; Complex1_49kDa; 2.
DR Pfam; PF00374; NiFeSe_Hases; 1.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR SUPFAM; SSF143243; Nqo5-like; 1.
DR PROSITE; PS00535; COMPLEX1_49K; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|PIRSR:PIRSR601501-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000029986}.
FT DOMAIN 35..152
FT /note="NADH:ubiquinone oxidoreductase 30kDa subunit"
FT /evidence="ECO:0000259|Pfam:PF00329"
FT DOMAIN 293..462
FT /note="NADH-quinone oxidoreductase subunit D"
FT /evidence="ECO:0000259|Pfam:PF00346"
FT DOMAIN 463..535
FT /note="NADH-quinone oxidoreductase subunit D"
FT /evidence="ECO:0000259|Pfam:PF00346"
FT BINDING 219
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 239
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 242
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 242
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 529
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 532
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 567 AA; 64533 MW; 4DBA5759A7AC70A5 CRC64;
MNQQKTGQHY IQELRQQFPS AILDESWQTD TQATITIKTN MLPEVVEFLY YKQGGWLSVL
FGNDERPLCG SYAVYYVLSM ETGTKCWITV KALVDERTLE FPSVTPRVPA AVWGEREVRD
MYGLIPVGLP DERRLVLPDD WPDELYPLRK DSMDYRQRPA PTTDVETYPF INESKSGKIV
PIGPLHITSD EPGHFRLFVD GEDIVDADYR LFYVHRGMEK LAETRMGYNE VTFLSDRVCG
ICGFAHSTAY TSSVENAMGI QVPERAQAIR SILLEVERLH SHLLNLGLSC HFVGFDSGFM
QFFRVREKSM KMAEILTGAR KTYGLNLIGG IRRDLLKDDM IQTRKLAAEM RAEVKTLVDI
LFSTPNMEQR TVGVGRLDPQ IARDFSNVGP MIRASGHARD TRVDHPFAGY DLLPMEIHTE
TGCDVISRVK VRVNEVFTAL NMIDFGLDNL PGGPLAVEGF TYIPNRFALG FSEAPRGDDI
HWSMTGDNQK LYRWRCRAAT YSNWPTLRYM LRGNTVSDAP LIIGSLDPCY SCTDRVTLVD
VRKRKSVTVP YKEIERYGLE RKDSPLK
//
