ID A0A098QZP7_9SPIO Unreviewed; 774 AA.
AC A0A098QZP7;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=DC28_04125 {ECO:0000313|EMBL:KGE73325.1};
OS Spirochaeta lutea.
OC Bacteria; Spirochaetota; Spirochaetia; Spirochaetales; Spirochaetaceae;
OC Spirochaeta.
OX NCBI_TaxID=1480694 {ECO:0000313|EMBL:KGE73325.1, ECO:0000313|Proteomes:UP000029692};
RN [1] {ECO:0000313|EMBL:KGE73325.1, ECO:0000313|Proteomes:UP000029692}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JC230 {ECO:0000313|EMBL:KGE73325.1,
RC ECO:0000313|Proteomes:UP000029692};
RA Shivani Y., Subhash Y., Tushar L., Sasikala C., Ramana C.V.;
RT "De novo Genome Sequence of Spirocheata sp.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase.
CC {ECO:0000256|PIRNR:PIRNR005536}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGE73325.1}.
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DR EMBL; JNUP01000031; KGE73325.1; -; Genomic_DNA.
DR RefSeq; WP_052078432.1; NZ_JNUP01000031.1.
DR AlphaFoldDB; A0A098QZP7; -.
DR STRING; 1480694.DC28_04125; -.
DR eggNOG; COG3345; Bacteria.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000029692; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000029692}.
FT DOMAIN 97..322
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 688..771
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT REGION 44..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 515
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 576
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT BINDING 236
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 403..404
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 480
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 513..517
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 554
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 576
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
SQ SEQUENCE 774 AA; 85974 MW; 249FD49D642C4ECC CRC64;
MIQLHDRQAI IHAGPLTYSL GATPEGFLLQ GFAGYLGSGV HSFQGDSNAS ASEASPDPSG
QNLPFQYLGP SARKAYHPPR PNSCAGQIYA PDIEPAQALM PPDLGPDSEL WEFPAPGIGD
FRNPGIEIRT GEGHTTCDLR VRSMRLLDEK PAIKGLPSVR QNPLEGDRTL EILLEDRVLS
LEIRLLYTSL PEYGVIARSA RVHNKGSRVL HLGHLASLNL DLPIHDYYFG QLSGTWARER
HPLFRPLQPG LTEVHSRRGQ SGPETSPQIF LAAPGTTETT GEVFSAGLVY SGNFSGSAEV
NGFGATRLQI GINPRGFDWP LAPGESFQAP EAWLGYSNRG FGPLSRNLHR LIRERLCISP
WSSRERPILL NNWEATYFDF SHKDLVTLAK AGAKAGIELF VLDDGWFGER RNDSSSLGDW
EVNPQKLPRG LEGLAGDIRA LGLEFGLWIE PEMVSPDSQL YRRHPDWCIH IPGRNRTLGR
NQLVLDLARR EVQDWIIHTV EGLVSKAPIT YIKWDMNRPI TESSPAHSHS YMLGLYRILE
HLTLGHPEVL FEGCSAGGGR FDLGMLAYYP QFWTSDDTDA IERLPIQWGT SLGYPTSAMA
SHISAVPNHQ VGRTTPLDTR AVTAFWGVFG YELNLSRSKD EELTQLNTLS GWYKKLRTLL
QFGDLYRLEG PTARPLGCSS PGALDRWSWM MTSRDRGRAL VCSVQVLSQP NPTVPRFLRL
EGLNAKTMYR VWAVSPRGQE DLGLVPGEVA LYRGIALPYR PGDFQSAMLY LEES
//
