ID A0A099KJ44_9GAMM Unreviewed; 791 AA.
AC A0A099KJ44;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Subtilisin {ECO:0008006|Google:ProtNLM};
GN ORFNames=ND16A_1980 {ECO:0000313|EMBL:KGJ90250.1};
OS Thalassotalea sp. ND16A.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC Colwelliaceae; Thalassotalea.
OX NCBI_TaxID=1535422 {ECO:0000313|EMBL:KGJ90250.1, ECO:0000313|Proteomes:UP000029848};
RN [1] {ECO:0000313|EMBL:KGJ90250.1, ECO:0000313|Proteomes:UP000029848}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ND16A {ECO:0000313|EMBL:KGJ90250.1,
RC ECO:0000313|Proteomes:UP000029848};
RA Stelling S.C., Techtmann S.M., Utturkar S.M., Alshibli N., Brown S.D.,
RA Hazen T.C.;
RT "Draft Genome Sequence of Thalassotalea sp. strain ND16A Isolated from
RT Eastern Mediterranean Deep Water.";
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the peptidase S8 family.
CC {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGJ90250.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JQDZ01000104; KGJ90250.1; -; Genomic_DNA.
DR RefSeq; WP_033078599.1; NZ_JQDZ01000104.1.
DR AlphaFoldDB; A0A099KJ44; -.
DR STRING; 1535422.ND16A_1980; -.
DR PATRIC; fig|1535422.3.peg.3058; -.
DR eggNOG; COG1404; Bacteria.
DR OrthoDB; 9790784at2; -.
DR Proteomes; UP000029848; Unassembled WGS sequence.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04817; PA_VapT_like; 1.
DR CDD; cd07477; Peptidases_S8_Subtilisin_subset; 1.
DR Gene3D; 2.60.120.380; -; 2.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 2.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR007280; Peptidase_C_arc/bac.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034202; Subtilisin_Carlsberg-like.
DR PANTHER; PTHR43806:SF59; CEREVISIN-RELATED; 1.
DR PANTHER; PTHR43806; PEPTIDASE S8; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 2.
DR Pfam; PF04151; PPC; 2.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000029848};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 30..791
FT /note="Subtilisin"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5001948431"
FT DOMAIN 161..394
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 395..480
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT DOMAIN 491..553
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 599..664
FT /note="Peptidase C-terminal archaeal/bacterial"
FT /evidence="ECO:0000259|Pfam:PF04151"
FT DOMAIN 708..776
FT /note="Peptidase C-terminal archaeal/bacterial"
FT /evidence="ECO:0000259|Pfam:PF04151"
FT ACT_SITE 169
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 202
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 505
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 791 AA; 82826 MW; 0E276873DB684B24 CRC64;
MKANRLTPKR LLLSTLALSV MTATSMTQAS ELREVSQINE PSETITFAQW QSKQQQQRLD
KTDRLIVKFK DKHQGKSIPP GLAKKAGLAL KHEKILKNGH HIISFGQQKN MKDVKALINK
LKNHKSIEFI EPDHKRFLMA QNQPWGISNT QSDLLSDNDA SNMTVCIIDS GYQQDNPDLS
ANNATGTNNS GTGNWYQNGG SHGTHVAGTI AAVNNSEGVV GIMPNTNVNL HIVKVFNESG
WGYTGELVDA VDTCVNNGAQ VINMSLGGAG SSNTEKNALQ AAADAGILLV AASGNDGNST
MSYPASYDAV MAVGAVDQNG QHAEFSQYTS QVEIAAPGEA ILSTVAGDGR LGYITIGGTT
YGNDEVVPQT RYVQSGSNYV VTNVNGSASG VLASCTISGS SYSCSNVAGN ICLAERNDNQ
VGSNYPEINP AKACTDAGAA GVIVYSDSAR PGLQNPFLVD GSSDVNVPTV SINRSLAQQL
LSQLGSNVEL TVNDGQNYAY YNGTSMATPH VTGVAALAWS NNPSCTADDV RSALKSTAID
LETAGRDDKT GFGLAQAKAA SDLLAAGCGT TTPPPTGGNV LENGVAKTNL TANSSEELSF
TIEVPAGSTD LSFDMSGGTG DADLYVKFGS APTSSSYDCR PYQSGNSESC PINNVQTGTY
YVNVIAYSTF SGVSLTANYT ESSTGGTGGA TGGSATVSDI SVARRAWQYY TIDVPAGMAT
LDFTMSGGTG DADMYIKQGA QPSSSSYDCR PYESGNNEFC SFNNPAATTW HIGIYGYRAA
SGVNLDVSYN P
//
