ID A0A099TVS9_9HELI Unreviewed; 586 AA.
AC A0A099TVS9;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE SubName: Full=Hydrogenase large chain {ECO:0000313|EMBL:STQ85263.1};
DE EC=1.12.5.1 {ECO:0000313|EMBL:STQ85263.1};
DE EC=1.12.7.2 {ECO:0000313|EMBL:STQ85263.1};
DE SubName: Full=Nickel-dependent hydrogenase large subunit {ECO:0000313|EMBL:TLE01342.1};
GN Name=hyaB {ECO:0000313|EMBL:STQ85263.1};
GN ORFNames=LS73_001260 {ECO:0000313|EMBL:TLE01342.1}, NCTC12714_00038
GN {ECO:0000313|EMBL:STQ85263.1};
OS Helicobacter muridarum.
OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=216 {ECO:0000313|EMBL:STQ85263.1, ECO:0000313|Proteomes:UP000255139};
RN [1] {ECO:0000313|EMBL:TLE01342.1, ECO:0000313|Proteomes:UP000029922}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ST1 {ECO:0000313|EMBL:TLE01342.1,
RC ECO:0000313|Proteomes:UP000029922};
RX PubMed=25428971;
RA Sheh A., Shen Z., Fox J.G.;
RT "Draft genome sequences of eight enterohepatic helicobacter species
RT isolated from both laboratory and wild rodents.";
RL Genome Announc. 2:e01218-e01214(2014).
RN [2] {ECO:0000313|EMBL:STQ85263.1, ECO:0000313|Proteomes:UP000255139}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC12714 {ECO:0000313|EMBL:STQ85263.1,
RC ECO:0000313|Proteomes:UP000255139};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|ARBA:ARBA00001967,
CC ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC {ECO:0000256|ARBA:ARBA00011771}.
CC -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC family. {ECO:0000256|ARBA:ARBA00009292}.
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DR EMBL; UGJE01000002; STQ85263.1; -; Genomic_DNA.
DR EMBL; JRPD02000002; TLE01342.1; -; Genomic_DNA.
DR RefSeq; WP_034559085.1; NZ_UGJE01000002.1.
DR AlphaFoldDB; A0A099TVS9; -.
DR STRING; 216.LS73_08500; -.
DR OrthoDB; 9761717at2; -.
DR Proteomes; UP000029922; Unassembled WGS sequence.
DR Proteomes; UP000255139; Unassembled WGS sequence.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0047067; F:hydrogen:quinone oxidoreductase activity; IEA:UniProtKB-EC.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR42958; HYDROGENASE-2 LARGE CHAIN; 1.
DR PANTHER; PTHR42958:SF2; UPTAKE HYDROGENASE LARGE SUBUNIT; 1.
DR Pfam; PF00374; NiFeSe_Hases; 1.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|PIRSR:PIRSR601501-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:STQ85263.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000255139}.
FT BINDING 43
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 62
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 65
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 65
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 559
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 562
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 565
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 586 AA; 65169 MW; 2AD974955D15C7D7 CRC64;
MSKRIVVDPI TRIEGHLRIE VVVDDNNVIT DAFSSSTLWR GLETIIKGRD PRDAGFIAGR
ICGVCTYSHY KAGITAAENA LGIEIPYNAK LVRTLMNLAL FFHDHIVHFY TLHGLDWCDI
ISALSANPAK ASKLAFEYTD NPIATGEDEL KMVQQRVKDF ASKGSLGPFA NGYWGHSTYH
FSPEQNLIVL SHYLKLLEVQ REAAKMMAIF GAKQPHPQSL TVGGVTSIMD ILDPTRLAEY
KFKFETVADF INRAYYPDLI MAGMAYSKEH SVLVGCNLKN FLSFQEIQVA HDEYLLSSGY
VLDGDISKVD DVNLDLIAEE VTNSWYKYEG KHSDGTPIKP TDLLHPYDGQ TTPNYIGMQD
GMSIGPSGKE EKTKLLKTDS KYSWIKSPRY DGKPMEVGPL ASIVVGLARK NPHIQEVAED
FLKRTGLTTA HLFSTLGRTA ARGLEAKVLG DYGLKAFDEL VKNLKVDTTT CAPYVIDKNK
EYKGYYIGNV PRGMLSHWMR ISDGKVSNYQ AVVPSTWNAG PRDSKGQKGP YEMSLIGTKI
ASLTQPLEII RHIHSFDPCI ACSVHVMDIS GNELGQYKVD PSFAKF
//
