ID A0A099ZAL4_TINGU Unreviewed; 356 AA.
AC A0A099ZAL4;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Lactosylceramide 4-alpha-galactosyltransferase {ECO:0000256|ARBA:ARBA00040835};
DE EC=2.4.1.228 {ECO:0000256|ARBA:ARBA00039051};
DE AltName: Full=Alpha-1,4-N-acetylglucosaminyltransferase {ECO:0000256|ARBA:ARBA00043186};
DE AltName: Full=Alpha-1,4-galactosyltransferase {ECO:0000256|ARBA:ARBA00041849};
DE AltName: Full=Globotriaosylceramide synthase {ECO:0000256|ARBA:ARBA00043154};
DE AltName: Full=UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase {ECO:0000256|ARBA:ARBA00041556};
DE Flags: Fragment;
GN ORFNames=N309_00758 {ECO:0000313|EMBL:KGL78028.1};
OS Tinamus guttatus (White-throated tinamou).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Palaeognathae; Tinamiformes; Tinamidae; Tinamus.
OX NCBI_TaxID=94827 {ECO:0000313|EMBL:KGL78028.1, ECO:0000313|Proteomes:UP000053641};
RN [1] {ECO:0000313|EMBL:KGL78028.1, ECO:0000313|Proteomes:UP000053641}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_N309 {ECO:0000313|EMBL:KGL78028.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP-
CC alpha-D-galactose = a globoside Gb3Cer (d18:1(4E)) + H(+) + UDP;
CC Xref=Rhea:RHEA:11924, ChEBI:CHEBI:15378, ChEBI:CHEBI:17950,
CC ChEBI:CHEBI:18313, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914;
CC EC=2.4.1.228; Evidence={ECO:0000256|ARBA:ARBA00043770};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11925;
CC Evidence={ECO:0000256|ARBA:ARBA00043770};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-Gal-(1<->1')-Cer + UDP-alpha-D-galactose = alpha-D-Gal-
CC (1->4)-beta-D-Gal-(1<->1')-Cer + H(+) + UDP; Xref=Rhea:RHEA:60044,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914,
CC ChEBI:CHEBI:143593, ChEBI:CHEBI:143594;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60045;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC -!- PATHWAY: Glycolipid biosynthesis. {ECO:0000256|ARBA:ARBA00004934}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KL890682; KGL78028.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A099ZAL4; -.
DR STRING; 94827.A0A099ZAL4; -.
DR Proteomes; UP000053641; Unassembled WGS sequence.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProt.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.20; -; 1.
DR InterPro; IPR007652; A1-4-GlycosylTfrase_dom.
DR InterPro; IPR007577; GlycoTrfase_DXD_sugar-bd_CS.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR12042:SF17; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE; 1.
DR PANTHER; PTHR12042; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE; 1.
DR Pfam; PF04572; Gb3_synth; 1.
DR Pfam; PF04488; Gly_transf_sug; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 4: Predicted;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676,
KW ECO:0000313|EMBL:KGL78028.1};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000053641};
KW Transferase {ECO:0000313|EMBL:KGL78028.1};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 227..352
FT /note="Alpha 1,4-glycosyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04572"
FT NON_TER 356
FT /evidence="ECO:0000313|EMBL:KGL78028.1"
SQ SEQUENCE 356 AA; 40970 MW; 2BE079FA9A63EB53 CRC64;
MRRMPGCLLK LTRVMLNHRL GALFIIVFKF LSFASILLYW RATEDPKVRG QLYVSPIEIH
CARSVPSPPR AAAEGPPPPP GDVFFVETSE RTNPNYLFLC SVESAARTHP GTRVVVLMKG
LAKSNASLPN HWGFSLLSCF PNVEIRPLDL QELFSGTPLA EWYLQAQQRW EPYFLPILSD
ACRIAIMWKF GGIYLDTDFI VLKNLKNLTN VLGTQSKYVL NGAFLSFKPK HKFMELCMQD
FVENYNSWIW GHQGPQLLTR VFKKWCSIRS LRSSRSCKGV SALPREAFYP IRWQDWKKYF
EVVSSSELHQ LFKNTYAVHV WNKKSQGTRL EITSKALLAQ LHSHFCPATY DIMKKD
//
