ID A0A0A0AWP2_CHAVO Unreviewed; 269 AA.
AC A0A0A0AWP2;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 22-FEB-2023, entry version 22.
DE RecName: Full=m7GpppX diphosphatase {ECO:0000256|ARBA:ARBA00015636};
DE EC=3.6.1.59 {ECO:0000256|ARBA:ARBA00012520};
DE AltName: Full=DCS-1 {ECO:0000256|ARBA:ARBA00032946};
DE AltName: Full=Decapping scavenger enzyme {ECO:0000256|ARBA:ARBA00030609};
DE AltName: Full=Hint-related 7meGMP-directed hydrolase {ECO:0000256|ARBA:ARBA00030789};
DE AltName: Full=Histidine triad nucleotide-binding protein 5 {ECO:0000256|ARBA:ARBA00030042};
DE AltName: Full=Histidine triad protein member 5 {ECO:0000256|ARBA:ARBA00030830};
DE AltName: Full=Scavenger mRNA-decapping enzyme DcpS {ECO:0000256|ARBA:ARBA00029885};
DE Flags: Fragment;
GN ORFNames=N301_15878 {ECO:0000313|EMBL:KGL97918.1};
OS Charadrius vociferus (Killdeer) (Aegialitis vocifera).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Charadriiformes; Charadriidae; Charadrius.
OX NCBI_TaxID=50402 {ECO:0000313|EMBL:KGL97918.1, ECO:0000313|Proteomes:UP000053858};
RN [1] {ECO:0000313|EMBL:KGL97918.1, ECO:0000313|Proteomes:UP000053858}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_N301 {ECO:0000313|EMBL:KGL97918.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside
CC in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + 2 H(+) +
CC N(7)-methyl-GMP; Xref=Rhea:RHEA:65388, Rhea:RHEA-COMP:17165,
CC Rhea:RHEA-COMP:17167, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58285, ChEBI:CHEBI:156461, ChEBI:CHEBI:167616;
CC EC=3.6.1.59; Evidence={ECO:0000256|ARBA:ARBA00024271};
CC -!- SUBUNIT: Homodimer. Associates with components of the exosome
CC multienzyme ribonuclease complex, such as EXOSC3 and EXOSC4. Interacts
CC with NDOR1. {ECO:0000256|ARBA:ARBA00011140}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the HIT family. {ECO:0000256|ARBA:ARBA00010208}.
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DR EMBL; KL873129; KGL97918.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0A0AWP2; -.
DR STRING; 50402.A0A0A0AWP2; -.
DR Proteomes; UP000053858; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0140932; F:5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IEA:InterPro.
DR Gene3D; 3.30.428.10; HIT-like; 1.
DR Gene3D; 3.30.200.40; Scavenger mRNA decapping enzyme, N-terminal domain; 1.
DR InterPro; IPR008594; DcpS/DCS2.
DR InterPro; IPR019808; Histidine_triad_CS.
DR InterPro; IPR036265; HIT-like_sf.
DR InterPro; IPR011145; Scavenger_mRNA_decap_enz_N.
DR PANTHER; PTHR12978; HISTIDINE TRIAD HIT PROTEIN MEMBER; 1.
DR PANTHER; PTHR12978:SF0; M7GPPPX DIPHOSPHATASE; 1.
DR Pfam; PF05652; DcpS; 1.
DR Pfam; PF11969; DcpS_C; 1.
DR PIRSF; PIRSF028973; Scavenger_mRNA_decap_enz; 1.
DR SUPFAM; SSF54197; HIT-like; 1.
DR SUPFAM; SSF102860; mRNA decapping enzyme DcpS N-terminal domain; 1.
DR PROSITE; PS00892; HIT_1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000053858}.
FT ACT_SITE 209
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-1"
FT BINDING 107
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT BINDING 117
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT BINDING 137
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT BINDING 139
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT BINDING 200..211
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KGL97918.1"
FT NON_TER 269
FT /evidence="ECO:0000313|EMBL:KGL97918.1"
SQ SEQUENCE 269 AA; 31402 MW; 4E0F3A403F06A51E CRC64;
QVTSPSGEGT DAVVILEKTP FQEEKVLDLL KKHTKLELQM RNDIYSTYHL YPPPELSEIK
TTVVYPATEK HLQKYLRQEV HLIRETWEDY KNITLPFIQS QSFSIQWVYN ILEKKAEADR
IVHENPDPCN GFVLVPDLKW NQNQLDDLYL IALIHRREVK SLRDLTAEHL LLLRNILQEG
KEAITKRFGV PGSQLRIYLH YQPSYYHLHV HFTALGYDAP GSSVERAHLL ADVIDNLAMD
SMYYQKRALT FALRADELLF KKFQEAGRV
//
