ID A0A0A0BAD0_9CELL Unreviewed; 724 AA.
AC A0A0A0BAD0;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=3'-phosphate/5'-hydroxy nucleic acid ligase {ECO:0000256|ARBA:ARBA00012726};
DE EC=6.5.1.8 {ECO:0000256|ARBA:ARBA00012726};
GN ORFNames=Q760_09665 {ECO:0000313|EMBL:KGM03062.1};
OS Cellulomonas cellasea DSM 20118.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Cellulomonadaceae;
OC Cellulomonas.
OX NCBI_TaxID=1408250 {ECO:0000313|EMBL:KGM03062.1, ECO:0000313|Proteomes:UP000029833};
RN [1] {ECO:0000313|EMBL:KGM03062.1, ECO:0000313|Proteomes:UP000029833}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20118 {ECO:0000313|EMBL:KGM03062.1,
RC ECO:0000313|Proteomes:UP000029833};
RA Wang G., Zhuang W.;
RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end
CC dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-
CC ribonucleotide-RNA + diphosphate + GMP + H(+); Xref=Rhea:RHEA:68080,
CC Rhea:RHEA-COMP:10464, Rhea:RHEA-COMP:13936, Rhea:RHEA-COMP:17355,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:37565, ChEBI:CHEBI:58115, ChEBI:CHEBI:83064,
CC ChEBI:CHEBI:138284, ChEBI:CHEBI:173118; EC=6.5.1.8;
CC Evidence={ECO:0000256|ARBA:ARBA00029282};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-
CC ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA +
CC diphosphate + GMP; Xref=Rhea:RHEA:68076, Rhea:RHEA-COMP:10463,
CC Rhea:RHEA-COMP:13936, Rhea:RHEA-COMP:17355, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:37565, ChEBI:CHEBI:58115, ChEBI:CHEBI:83062,
CC ChEBI:CHEBI:138284, ChEBI:CHEBI:173118; EC=6.5.1.8;
CC Evidence={ECO:0000256|ARBA:ARBA00029311};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|PIRSR:PIRSR601233-3};
CC Note=Binds 2 manganese ions per subunit.
CC {ECO:0000256|PIRSR:PIRSR601233-3};
CC -!- SIMILARITY: Belongs to the RtcB family.
CC {ECO:0000256|ARBA:ARBA00008071}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGM03062.1}.
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DR EMBL; AXNT01000026; KGM03062.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0A0BAD0; -.
DR STRING; 1408250.Q760_09665; -.
DR Proteomes; UP000029833; Unassembled WGS sequence.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008452; F:RNA ligase activity; IEA:InterPro.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.90.1860.10; tRNA-splicing ligase RtcB; 2.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR001233; RtcB.
DR InterPro; IPR036025; RtcB-like_sf.
DR PANTHER; PTHR43749; RNA-SPLICING LIGASE RTCB; 1.
DR PANTHER; PTHR43749:SF2; RNA-SPLICING LIGASE RTCB; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR Pfam; PF01139; RtcB; 2.
DR PRINTS; PR00379; INTEIN.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF103365; Hypothetical protein PH1602; 2.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134, ECO:0000256|PIRSR:PIRSR601233-
KW 2}; Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|PIRSR:PIRSR601233-3};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR601233-3};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRSR:PIRSR601233-2};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Reference proteome {ECO:0000313|Proteomes:UP000029833};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 390..503
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT BINDING 78
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR601233-3"
FT BINDING 173..177
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0000256|PIRSR:PIRSR601233-2"
FT BINDING 174
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR601233-3"
FT BINDING 191
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601233-3"
SQ SEQUENCE 724 AA; 78965 MW; C5891D329BCE9510 CRC64;
MMSSPFPVAL AGTAAPTLMW AHEHEVEPQA LQQLRNIASL PWVEGLRVMP DVHLGKGATV
GSVIAMRDAV SPNAVGVDIG CGMIGVRTSL TAADLPDDLH AIRTRIEQAV PVGFHAHDEP
VDLRRLRPVN GSAGRERLKG ADAFWDRFGG LHRTVQQLEA RARKQLGTLG GGNHFIELCL
DESDQVWLQL HSGSRNIGKE LAERHVAIAK TLEHNQRIVD RELAVFLAGT PQMDAYLNDL
WWAQEYAARS RAVMMALVVQ AVRDSFPERE ITFDEGVNCF AGETRVLTGA GIFPIAELAG
GIHELLTTGG RWVKAPIMSF GKQRVYEVTV GRYGEEKVIR ATGNHRWLLR AKVAHARDEA
TTQDLRVGDR LAYAFPARVS GMKVDRASVA RGFVFGDGSL CGKQTRARAI FCGDKDESLL
PYFEGLTTNC VRDYGSVKVL NGFPAEWKTA PVATSSHPDI VYGWLAGYFA ADGDVDKSGR
PSLSSSRRDH LEAVKALATS IGVGTYGIRT RVRRGIDGRD SELHVMGFMR SDLDLDFFVQ
DEHRARFATG RGAVERKGWT VRSVEITDDV EEVYCAVVPE TEAFTLEDNI LTRNCHHNYV
KTEQIDGAEL IVTRKGAIRA GSGEMGLIPG SMGTGSYVVR GLGNPASFQS ASHGAGRRMS
RTAAKKRFTV EDLAAQTAGV ECRKDAGVVD EIPGAYKDLE SVIAAQTDLV EVVARLRTIV
CVKG
//