ID   A0A0A1T7H5_9HYPO        Unreviewed;       601 AA.
AC   A0A0A1T7H5;
DT   04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT   04-FEB-2015, sequence version 1.
DT   27-MAR-2024, entry version 31.
DE   SubName: Full=Putative tRNA pseudouridine synthase {ECO:0000313|EMBL:CEJ82105.1};
GN   ORFNames=VHEMI02193 {ECO:0000313|EMBL:CEJ82105.1};
OS   [Torrubiella] hemipterigena.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae;
OC   Clavicipitaceae incertae sedis; 'Torrubiella' clade.
OX   NCBI_TaxID=1531966 {ECO:0000313|EMBL:CEJ82105.1, ECO:0000313|Proteomes:UP000039046};
RN   [1] {ECO:0000313|EMBL:CEJ82105.1, ECO:0000313|Proteomes:UP000039046}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Horn F., Habel A., Scharf D.H., Dworschak J., Brakhage A.A., Guthke R.,
RA   Hertweck C., Linde J.;
RT   "Draft Genome Sequence and Gene Annotation of the Entomopathogenic Fungus
RT   Verticillium hemipterigenum.";
RL   Genome Announc. 3:e01439-e01414(2015).
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000256|ARBA:ARBA00009375}.
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DR   EMBL; CDHN01000001; CEJ82105.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0A1T7H5; -.
DR   HOGENOM; CLU_021971_1_0_1; -.
DR   OrthoDB; 12038at2759; -.
DR   Proteomes; UP000039046; Unassembled WGS sequence.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR   CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR   Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR041708; PUS1/PUS2-like.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   NCBIfam; TIGR00071; hisT_truA; 1.
DR   PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR   PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   Reference proteome {ECO:0000313|Proteomes:UP000039046};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT   DOMAIN          394..497
FT                   /note="Pseudouridine synthase I TruA alpha/beta"
FT                   /evidence="ECO:0000259|Pfam:PF01416"
FT   REGION          1..67
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          296..350
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          573..601
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        48..67
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        296..314
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        587..601
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        163
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT   BINDING         219
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ   SEQUENCE   601 AA;  66956 MW;  32CED330B8735CAE CRC64;
     MAADGESSAA GSSSNSGGHA RHQGGRGGRG GGRGGRGGNN RGKKGGDLGR SAWNRQKVEK
     RKETVDYREF KRRKVNAQGE SIEVERDNPF SKDEIANEER RPKRKVAVMI GYSGTGYKGM
     QVNGDEATIE RDLFQAFIKA GAISKANADD PRKSSLARCA RTDKGVHAAG NVISLKLIIE
     DEDIIDKINE ALPEQIRVWG IQRTNNSFNC YQYCDSRFYE YLMPSYCLLP PHPNSFLGQK
     LVELTKEHGA EEQVAERLDD VKDFWQNVEE TAIKPILAKL DPEVRAQVLE RVHALEEDVE
     NESKPAEESK PAETTEAASE QPVAEGTEAE APAPTAAEGE EAKNVTLTSV KPKGRELGPV
     DFALRDIKAA YISAKRAYRV TPARLKQLQD GLDKYVGTNS FHNYTVLKTH GDPSAKRHIK
     SFIVNPKPVI IGNTEWLSLR VHGQSFMMHQ IRKMVGLISL MVRCGTPWDR VDESYGKVKM
     AIPKAPGLGL LLERPVFENY NRRATQNLGR EAIDFDKYSD RMDAFKQKEI YQRIFDVEEK
     DNSFHTFFNQ IDQFKSNHFL WLTAGGLKAA VIAPNETGKS EEDVSKQLGD EDEEDPDGGQ
     G
//