ID A0A0A1V471_9HYPO Unreviewed; 3883 AA.
AC A0A0A1V471;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE SubName: Full=Hybrid polyketide synthase-nonribosomal peptide synthetase {ECO:0000313|EMBL:EXV04383.1};
GN ORFNames=X797_002056 {ECO:0000313|EMBL:EXV04383.1};
OS Metarhizium robertsii.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX NCBI_TaxID=568076 {ECO:0000313|EMBL:EXV04383.1, ECO:0000313|Proteomes:UP000030151};
RN [1] {ECO:0000313|EMBL:EXV04383.1, ECO:0000313|Proteomes:UP000030151}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ARSEF 2575 {ECO:0000313|EMBL:EXV04383.1,
RC ECO:0000313|Proteomes:UP000030151};
RA Giuliano Garisto Donzelli B., Roe B.A., Macmil S.L., Krasnoff S.B.,
RA Gibson D.M.;
RT "The genome sequence of the entomopathogenic fungus Metarhizium robertsii
RT ARSEF 2575.";
RL Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000256|ARBA:ARBA00004685}.
CC -!- SIMILARITY: Belongs to the NRP synthetase family.
CC {ECO:0000256|ARBA:ARBA00029454}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the NRP synthetase
CC family. {ECO:0000256|ARBA:ARBA00029443}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EXV04383.1}.
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DR EMBL; JELW01000002; EXV04383.1; -; Genomic_DNA.
DR eggNOG; KOG1178; Eukaryota.
DR eggNOG; KOG1202; Eukaryota.
DR HOGENOM; CLU_000022_37_9_1; -.
DR OrthoDB; 5396558at2759; -.
DR Proteomes; UP000030151; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0046483; P:heterocycle metabolic process; IEA:UniProt.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:1901360; P:organic cyclic compound metabolic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd20483; C_PKS-NRPS; 1.
DR CDD; cd05195; enoyl_red; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 4.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR013120; Far_NAD-bd.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 1.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF13602; ADH_zinc_N_2; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF00668; Condensation; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF07993; NAD_binding_4; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 2.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF50129; GroES-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 3.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 10..434
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2232..2309
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3366..3440
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 2315..2339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2318..2333
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3883 AA; 427287 MW; 14B9E5F30CE8B086 CRC64;
MASRINGRAD DPSVIVGLAC RVPGANTPSK LWQSIAEQRD VQQKMPEDRL KVDSFFHPEP
THKGTTNARF GYFLDQDIGN FDAGFFGISG KEAEAMDPQQ RLLLEVVYEA LENAGITLQE
IRGSLTSVFC GSFTNDYNAM LTKDLEYYPK YTVTGTGDAI LSNRISYFYD LHGTSVTIDT
ACSSSLVCFH LGSQTLQNGE ADISIVVGSS LHYDSNVFVT MTDLGMLSTN GRCAAFDASG
SGYVRGEGIA CAILKRKSDA IANGDSIRAV VRATGSNHDG KKNGITLPNS VAQEQLIRST
YERAGLNPSH TQYFEAHGTG TAAGDPIEAR AIGAVFAPGR DEPLFVGSVK TNIGHLEGAS
GLAGVIKTTM ALEKNYIPPN MHFKTPNPNI DFEKWKITVP TKLVEWKVPD GVPRRASINS
FGYGGTNAHV VLEEYTQNEQ IPAAIPPEPV ANGVHSRPYL VPLTSHSPKA GEMSEETLKA
YLNNTEDATI ADLAYSLSTR RTLHQQRSFV VANDKAGLVD QLSCPRPAAP WAIAKNPVPR
LGFIFTGQGA QWFAMGRQLI QECPHFRQSL QRCDAILKSL PDAPEWSIVA ELNKTKETTL
LGETLYSQTI CTALQLAIVD LIECWGIKPS AVVGHSSGEM GAAYSAGILS FESALIAAYY
RGRYMSSSRS DGVPGGMMAV GLPENKCLEE LKPYAGRLTI AAVNSPSTMT VSGDQDAILE
LKEKLTEKKV FVRQLVVKQA FHSHHMFPLA PAYENALKNS PAFKTNPPTC RMFSSVTCRR
ADHEKMGASY WSANMVQAVR FSDALTGILL DEEDEQNVDI LVEIGPHPAL KGPARQTVQS
LKLDLPYVAS LTRGVPDFEG LLNMAGTLFS LGYPVDIVAA NQNLSQTSHG ALVSTPTGTK
LHDLPTYTWE HRRYWSETRY IKEHRQREFR HATLGHRVAG SVVRHPLFRN YLRLSELPWL
NEHVVENKVV FPGAGYISMA IEAAIRTDRA ESVKMIHLKD IVVKNALLIP STDEGVEVLL
QLKPVTLSAK SHSDTWYEFN VFSYDENSNC TSHCYGLVSV ERGDAAPVNY TCRYPEGTNL
NELRKKTYRS MPAGTFYKNM AELGLAYGDK FRLLKGSIES GVGFAVSDLV FDPGHLPLEA
GDETVLHPTL LDSFFHVIFH AVENCLGRPL DEPYVPSFFR ALRISGDFFH GRYSPAVKKF
QVASFTKLPS PRVAINDMIM QNDRGELMME IAGLEVTSLG REAPDGQGPR TLFYRQRWQP
FFDMMTKVEG KPVSDIVDVF AHQYPNTKML HITSDVNKTR DFIQHLGCGK GERRRFKQLD
VWSLLGNEFG EEAERLSKEC RGLVNVVEPA LDSYDLIIVS EAGVNAVPFM NDTGCILFDG
KKGVVTAELQ ELFSSPFCTA VRNAVEPFTL NSELSVVMPT CKPSARTSLI LKELESAHGG
DISFTSFPEL AHKGTRLLAK DVVVLAGLDE SISDKAAFKG AQALLTSLEK NVIWPTEGAT
LEASRPDNAM FIGLVRAARS ENDTLRAVNL DFGIDSPAQT VAANIVRLLD GRVTEDELTE
RNGIVYIPRV EADDERNCKL RNGPDQEPRL EPFGTAQTRT PLALRIGKVG LLETLYFGED
TQVMDTKIND NEIEVETKAS SINFRDVAAS MGIIEDSNLG DECAGICTKV GANVRGFQVG
DRVVALRPGQ GAHRSLVRNP ASWCYKLPDN MSYADAAAMP LILGTAWFAL DHTARLAKGE
SVLIHAAAGG VGQMAIQIAQ RAGAHIYATV GSPAKRQLLK DVYGLKEEQM FSSRDASFVE
GVMEATGGKG VDVVLNSLAG PLLHASWACL APFGRFLEIG KRDIHENTKI AMDPFRRNVL
FASIDLVTMF EKNEALGERL FKECFALLAN GEIRTPATIK EVPYSDVVKG FRLLQMGKHT
GKIVLVPHAE DMVPVMRGGY RDVKLFSSSK TYLLVGGLGG LGRTLSQWMV RKGARKLAFL
SRSGADKAEA KATIDWLIER GVDATVYRGD VSKFTDVRSC VDQIGSSLGG IFQAAMVLQD
KPLETMTYEQ WQHCCQPKVE GTKNLHQATL GNQLDFFVCF SSVAAVVGSK GQANYSAANC
YLDALMRHRR ELGLSGTTMN VGAVTGVGVV AENEELQKIM LRMGMDTINE EELLYQLEEA
VLADKFGHPV TARGCNGHQI ITGVGLISPD VYWASKPMMK NLYTNHDFGA GGAGQSSKNL
LALLSEEPDI EKKTEILLDG FLEKIASVLA TPRESILPSN PLSAYGLDSI VAVEFRKWFR
KEVQADIALF DILGAASINA LVSKTARMVT TISASKHTAG DKPEKKSAVE SEGTEEDSNI
SAMATGQLTK SQHSGVVPLS TFQSRLWFVH SFLEDKSFLN LPIVLRIRGK PDYPTLQKAV
QEMAVRNPAL RTAYFEGDDF AVQEPLEDFE LGVDFRDLTK EVDTEKALEE FVKYNRKIEM
NVEEGEVATY SLAKLSEEEW AVVGMIHHIS IDRASLFPIM SQFVGIYDAL RTGNDLATVS
APEFNYVDFT LWHNARLASN LMKPDLDWWR TTLKGIPQSS KLLPFAKSER PARSDPLRQK
VRTNLNAKLF ARMKRIASQS NGTPFHFVLA AFRAFLYRYT EDKDLVLLMV DGNRPHPDTD
PLMGFFVNLA PVRCNDDCDV PFDQLFQATK TRALDAMAHS GVPFDTIVDI MKVKKTSSHM
PVGQIAINYQ IHGPVPTYTT CDFVVENVES DDIPTAADIQ LEAIETSEHS MDLKIEYSTA
LYDNPDMERF LDNFNTFLES CIKDHRQPVD EISMCGPLEI EFLKKNYWNM ETKENQWGEQ
SVLDVITKMA RQHPQTAAIK TSDCRSMTYR QLIESAESVA SELLNAGAQP GDKVGLIALP
GLEAVTGMLA ALMTGSCYVA LDTDFAHDRL SFMLSDSGAR ILLTGPGQDG LVAELLSKMV
MAPKVIRIED AAAAGRTVAH RRARHPDDPF YMIYTSGSTG TPKGVVLRES NTQAMLATLN
KDYAFSHEDN FLAHTTTSFD LSIVQIFGGL TAGATVSVAS WETRKDPVAL ADFMMREGVS
VTYFTPTQFA LLMEFNSEAL RNCSKYRVAY FAGERLPVRV AKAFYDLGTP ATLYNTWSPS
ELVVQTSISK IDYPGDGVVS LPIGYPMDNC RHYLLDIKGN PVPAGQIGEL VVGGVQVGAG
YLNRPDVNAR SFVEDPFASE DDRKRGWNRM FKTGDRGRFR PDGQLEFHGR IAGDKQIKLR
GFRIDLGELE QVIFKESQSL KKAGALVDIA VVARTVDSDE QQLIAYLVPK ASITNEADKI
AIVSHLHRKI KPHLNYYMLP NGYQFLAKLP VTLGGKVDRR NLLERQLQLL FPSSITTQPG
NTASGEPTED LEGTILALFR TTLGREIELN DSFFERGGNS ILLVRLQAKV KKQFKIAPPL
PALIREPTAA AVCAYLRRAK GGDGADKGGL ASVISWNVET NLPNTSQYIP RYGIPRIDRD
ELKSILLTGA ESFVGIHLLA EILRTKRDAT VHVLGSMEKL EPQVLIHILQ KLDLLQDSLT
LDDINSRVKF VPGSLNQSNF GLSRSAFREL GQTVQSIYHL GGHVSLLKTY TSLKASNVTP
IFDIIKLSGL GSHLSDINYL STWSVAHLQT WSASKRTRED YATGEEDSSH FSPPTEDESG
YFKTRWVAEN LLTKAARRGF PVTITRASGV TAAVRGPGVP DPGDEFIMRI VVGMIESGMV
PQIGRADQPS FAVDVVPVDW LASNLFALTS EKEALSRVAP SEVYSAPRIY HITNPLPLRL
KDLPRIIAEL RSEQTRPRMV SLDEWLDSME SAEGEDAAGR VVRSTVLKQY LSTGGVMFSL
DNRKTMDILD GLHPGVAAGC PAVDAEFLRA LLERLKGGEK FSS
//
