ID A0A0A2TJN9_9FIRM Unreviewed; 524 AA.
AC A0A0A2TJN9;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
GN ORFNames=JT05_08535 {ECO:0000313|EMBL:KGP75844.1};
OS Desulfosporosinus sp. Tol-M.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC Desulfosporosinus.
OX NCBI_TaxID=1536651 {ECO:0000313|EMBL:KGP75844.1, ECO:0000313|Proteomes:UP000030439};
RN [1] {ECO:0000313|EMBL:KGP75844.1, ECO:0000313|Proteomes:UP000030439}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Abu Laban N., Tan B., Dao A., Foght J.;
RT "Draft genome of Desulfosporosinus sp. Tol-M obtained by stable isotope
RT probing of toluene- degrading methanogenic culture enriched from oil sands
RT tailing of Alberta, Canada.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|RuleBase:RU003862};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGP75844.1}.
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DR EMBL; JQID01000115; KGP75844.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0A2TJN9; -.
DR STRING; 1536651.JT05_08535; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000030439; Unassembled WGS sequence.
DR GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00537; MTHFR; 1.
DR Gene3D; 3.20.20.220; -; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR InterPro; IPR022026; MTHFR_C.
DR PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF12225; DUF5981; 1.
DR Pfam; PF02219; MTHFR; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU003862};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003862};
KW Reference proteome {ECO:0000313|Proteomes:UP000030439}.
FT DOMAIN 396..488
FT /note="Methylene-tetrahydrofolate reductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF12225"
SQ SEQUENCE 524 AA; 58360 MW; 37ABCC7F57BB0AE4 CRC64;
MENKFKESLL DKNSLCVTWE LVPGRGANEK AQETAIASAE QAAKGGRVHA LTITDNPGGN
PAMLADYLGM EILKMGIEPL VHFTCKDKNR NQMESQLYAL DRAKVRNLLV MTGDYPVSGF
QGRPKPVFDL DPSNTLQLIT EMNKGLEVQG MKGPTYHAPS TFFAGAAVSP FKSTEAELMV
QYYKLKKKIE SGAQFIVMQL GYDVRKCHEV LQFLKINNLD IPVMGNIYVL PYGAANLMNK
NKLPGCVVTD KLLAELNTEK SAPDKGVGQR MLRAAKLYAI LKGMGVAGVH IGGHNLKYEQ
VEYILDKGEE LSPDWQDLVK EFDYPQTNGF YYFEKDELTG LNTDRPTNRA NLPLDAPTGA
MYTLLRGMHG LLFTPHKRLF PMMRGMYRAA EGKKKTEKGL HAIEHLVKVA MLDCKDCGDC
AILDLAYLCP MSQCPKNQRN GACGGSLDGW CEVYPQEKKC VWVRAYARLK KYGEEASLDV
PAVPPANWDF YQTSSWSNFF LGKDHSASRL GIKRPESKIK DGTV
//
