ID A0A0A2U050_9FIRM Unreviewed; 588 AA.
AC A0A0A2U050;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:KGP74875.1};
GN ORFNames=JT05_14040 {ECO:0000313|EMBL:KGP74875.1};
OS Desulfosporosinus sp. Tol-M.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC Desulfosporosinus.
OX NCBI_TaxID=1536651 {ECO:0000313|EMBL:KGP74875.1, ECO:0000313|Proteomes:UP000030439};
RN [1] {ECO:0000313|EMBL:KGP74875.1, ECO:0000313|Proteomes:UP000030439}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Abu Laban N., Tan B., Dao A., Foght J.;
RT "Draft genome of Desulfosporosinus sp. Tol-M obtained by stable isotope
RT probing of toluene- degrading methanogenic culture enriched from oil sands
RT tailing of Alberta, Canada.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGP74875.1}.
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DR EMBL; JQID01000201; KGP74875.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0A2U050; -.
DR Proteomes; UP000030439; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR026447; B12_SAM_Ta0216.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04190; B12_SAM_Ta0216; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF1; TIGR04190 FAMILY B12-BINDING DOMAIN_RADICAL SAM DOMAIN PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00326; 5''-pyrrole_methytransferase; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000030439};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 26..162
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 218..452
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 588 AA; 67097 MW; 6ECD4D0CDC925A65 CRC64;
MRRDVIFLHA PSIYDFRKDT ILFGPINDVI PSSSVFEMYP IGITSIAEHL EKSGLNVQII
NMAYRMLKSP AYDPEKEIKK RHPRVWAIDL HWLPHVHGAL ALAALIKKHH PDVPVLMGGL
SSSYFHEELI KNPDVDFVVR GDSTEGPVLE LLTRVRAGLP LEGVPNLTWK QKDGSVVVNP
LTNVPDNLNA PTVPAYRYTM RSVFKYWNLS NIVPYLRWLE YPMTTILTAR GCNINCSICG
GSKQAYRQVC QRKKPAFRSP ERLIEDVRLI KRFSRAPIFV IHDLRMGGKE FADRFLYLLK
QEKIENEMVF ELFYPADEEF ISQLKDAALH FSLEMTLETH DPELRKTNGK FAVSNEAIEA
TIAAAMANGC NRLDIFFMVG LPFQTTQSVM DSMDYAHKLL SDYGLDGRIK VYVAPLAPFL
DPGSPAFEDP EKFGYKLRAH TLEEHRKLLT NSTWGQILNY ESMSMPPQEL VNTTYEATAK
LVASKNKLGL MSNEEATETT NLIIQAQYLN NKMEQAVLIK NKKERSLAIA ALKNEAKDVN
SKRVYAQTDF VSWGKQRFQL KPLGIISLLF ELFFEELSLA KLRYSKRR
//
