ID A0A0A2U2V4_9FIRM Unreviewed; 462 AA.
AC A0A0A2U2V4;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:KGP75885.1};
GN ORFNames=JT05_08050 {ECO:0000313|EMBL:KGP75885.1};
OS Desulfosporosinus sp. Tol-M.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC Desulfosporosinus.
OX NCBI_TaxID=1536651 {ECO:0000313|EMBL:KGP75885.1, ECO:0000313|Proteomes:UP000030439};
RN [1] {ECO:0000313|EMBL:KGP75885.1, ECO:0000313|Proteomes:UP000030439}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Abu Laban N., Tan B., Dao A., Foght J.;
RT "Draft genome of Desulfosporosinus sp. Tol-M obtained by stable isotope
RT probing of toluene- degrading methanogenic culture enriched from oil sands
RT tailing of Alberta, Canada.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGP75885.1}.
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DR EMBL; JQID01000113; KGP75885.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0A2U2V4; -.
DR STRING; 1536651.JT05_08050; -.
DR Proteomes; UP000030439; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000030439}.
FT DOMAIN 1..138
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 188..409
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 462 AA; 52849 MW; A18617D46B5CA934 CRC64;
MRILLVAYDN DSYISWFPQG LAYIASVCLK AGHEVKIYNQ DVYHWPESHL TDLLNKERYD
FIGVSMCGGY YQYRKVLKLS EAINKAKHRP FYVLGGHLAA PEPAYFLNKT SADAIVIGEG
EITVLNLLEA LEQKKPLHSV NGIAFLENGV CVQPPRQDLI EDIDGIPFPA WELFPIDHYA
LLRAPYIKNG ERWFPVLSGR GCTFKCNFCY RMDEGFRGRS PESIVEEIKI LQKDYMISFI
TFSDELLMNS AQRTVELCET FLKNKLNIKW DCNGRLNYAK PDVLKLMKEA GCVFINYGIE
SLDEQVLRNM NKALTVKQIV KGIENTSAAG LSPGFNIIFG NIGESAESLR LGVEFLLKYD
DHAQMRTIRP VTPYPGSSLY YYAIQKGLLK DCEDFYEQKH VNSDLLSVNF TDLSDAEFHR
VLYEANQKLL LNYYAAQITK TNEALHKLYL EKDSSFRGFR QS
//
