ID A0A0A3Z4D2_9GAMM Unreviewed; 398 AA.
AC A0A0A3Z4D2;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE SubName: Full=Mandelate racemase/muconate lactonizing protein {ECO:0000313|EMBL:KGT92481.1};
GN ORFNames=NG99_13875 {ECO:0000313|EMBL:KGT92481.1};
OS Erwinia typographi.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Erwinia.
OX NCBI_TaxID=371042 {ECO:0000313|EMBL:KGT92481.1, ECO:0000313|Proteomes:UP000030351};
RN [1] {ECO:0000313|EMBL:KGT92481.1, ECO:0000313|Proteomes:UP000030351}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M043b {ECO:0000313|EMBL:KGT92481.1,
RC ECO:0000313|Proteomes:UP000030351};
RA Chan K.-G., Tan W.-S.;
RT "Genome sequence of Erwinia typographi M043b.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR633978-3};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR633978-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGT92481.1}.
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DR EMBL; JRUQ01000040; KGT92481.1; -; Genomic_DNA.
DR RefSeq; WP_034893851.1; NZ_JRUQ01000040.1.
DR AlphaFoldDB; A0A0A3Z4D2; -.
DR STRING; 371042.NG99_13875; -.
DR eggNOG; COG4948; Bacteria.
DR OrthoDB; 9796450at2; -.
DR Proteomes; UP000030351; Unassembled WGS sequence.
DR GO; GO:0008867; F:galactarate dehydratase activity; IEA:InterPro.
DR GO; GO:1990594; F:L-altrarate dehydratase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009063; P:amino acid catabolic process; IEA:InterPro.
DR CDD; cd03316; MR_like; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR033978; L-talarate_dehydratase.
DR InterPro; IPR018110; Mandel_Rmase/mucon_lact_enz_CS.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR InterPro; IPR046945; RHMD-like.
DR PANTHER; PTHR13794; ENOLASE SUPERFAMILY, MANDELATE RACEMASE; 1.
DR PANTHER; PTHR13794:SF58; MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDS00001; Enolase; 1.
DR SFLD; SFLDF00134; L-talarate/galactarate_dehydra; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
DR PROSITE; PS00909; MR_MLE_2; 1.
PE 4: Predicted;
KW Magnesium {ECO:0000256|PIRSR:PIRSR633978-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR633978-3}.
FT DOMAIN 176..273
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
FT ACT_SITE 197
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-1"
FT ACT_SITE 328
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-1"
FT BINDING 46..48
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT BINDING 82..83
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT BINDING 195
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT BINDING 226
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-3"
FT BINDING 228
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT BINDING 252
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-3"
FT BINDING 278
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-3"
FT BINDING 348
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-2"
FT SITE 301
FT /note="Increases basicity of active site His"
FT /evidence="ECO:0000256|PIRSR:PIRSR633978-4"
SQ SEQUENCE 398 AA; 44053 MW; E8B00D2F35A1A049 CRC64;
MSLSANSEAV TYAKVAHAKT AAETGDRIEW VQLSLAFLPL ATPVSDAKVL TGRQKPLTEV
AIIIAEIRSR DGFEGVGFSY SKRAGGQGIY AHAKEIADNL LGEDPNDIDK IYTKLLWAGA
SVGRSGMAVQ AISPMDIALW DMKAKRAGLP LAKLLGAHRD SVQCYNTSGG FLHTPLEQVL
KNVVISRENG IGGIKLKVGQ PNCAEDIRRL TAVRQALGED FPLMVDANQQ WDRETAIRMG
RKMEQFNLIW IEEPLDAYDV EGHAQLAAAL DTPIATGEML TSFREHEQLI LGNASDFVQP
DAPRVGGISP FLKIMDLAAK HGRKLAPHFA MEVHLHLSAA YPLEPWLEHF EWLNPLFNEQ
LELRDGRMWI SDRHGLGFTL SEQARRWTQL SCEFGKRP
//
