ID A0A0A5FSV3_9BACI Unreviewed; 1175 AA.
AC A0A0A5FSV3;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 24-JAN-2024, entry version 41.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=N783_20945 {ECO:0000313|EMBL:KGX83856.1};
OS Pontibacillus marinus BH030004 = DSM 16465.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Pontibacillus.
OX NCBI_TaxID=1385511 {ECO:0000313|EMBL:KGX83856.1, ECO:0000313|Proteomes:UP000030403};
RN [1] {ECO:0000313|EMBL:KGX83856.1, ECO:0000313|Proteomes:UP000030403}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BH030004 {ECO:0000313|EMBL:KGX83856.1,
RC ECO:0000313|Proteomes:UP000030403};
RA Huang J., Wang G.;
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGX83856.1}.
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DR EMBL; AVPF01000079; KGX83856.1; -; Genomic_DNA.
DR RefSeq; WP_027447161.1; NZ_AVPF01000079.1.
DR AlphaFoldDB; A0A0A5FSV3; -.
DR STRING; 1385511.GCA_000425225_03592; -.
DR eggNOG; COG1197; Bacteria.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000030403; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000030403}.
FT DOMAIN 638..799
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 820..974
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1175 AA; 134816 MW; E9B06B89F6E37AB6 CRC64;
MQGLKDFLKK QDDVHSIISG FSSGMQEQFA AGLSGSARSV LVSLLEESTK RPILLVTHQL
MQAQQLYEDL LGISDAEHVH LYPVNELIAS EIAVASPELR SERIDALNHW AQEKSGILIA
PVAALKRILP PPSYWEQYQL NFKVGEAIDL DAYLKGLINV GYERADMVSS PGEFSLRGGI
LDIYPLTQPN PLRIELFDDE VDSIRYFDSE SQRSLEKLDQ VTIGPASELL LTDEDLTRGA
HRLEEALAKT LQKMQSSELK EKVTETIEHD ISRLNNHERF EEMYKYISLY YENSASLLDY
IPENGIVVLD EMSRIQETAN RLDEEEADWY SSLLESGQMV RDISISYNWP DVWHAIKQPR
LYMSVFLRHI PNTQPQNIVN FSCRAMQQFH GQMNLLQNEM DRWKKSGYSV IIVAPNHERA
QKIQNVLEDY DMEAVVSEHE PSLPVTTPTI VTEQMTSGFE LPMHKIAVLT ESELFKKQTT
RPKRKKKISN AERIKSYQEL KVGDYVVHAN HGIGKYIGIE TLELNGIHKD YMLVKYSGDD
KLYVPIDQID QVQKFVGSEG KEPKLYKLGG SEWKKVKSRV QSSVEDIADD LIQLYAEREA
TKGYAFGEDT EMQKDFEAAF PYQETEDQIR CIQEIKTDME KIRPMDRLLC GDVGYGKTEV
AIRAAFKAIA DGKQVAILVP TTILAQQHYE TVRERFQDFP INIGLLSRFR TQKQQKETKE
GLKSGIVDIV IGTHRLLSKD IQVKDLGLLI VDEEQRFGVK HKEKIKQMKT NVDVLTLTAT
PIPRTLHMSM LGVRDLSVIE TPPENRFPIQ TYVLEYNPVF MREAIEREMA RGGQVFFLFN
RVENIERMAE EISALVEDAR VTFAHGQMNE TELENVMFSF LEGESDVLVS TTIIETGVDI
PNVNTLIVYD ADRMGLSQLY QLRGRVGRSN RVAYAYFTYQ KDKVLTEVAE KRLQAIKEFT
ELGSGFKIAM RDLSIRGAGN LLGAQQHGFI DSVGFDMYSQ MLKDAIEAKQ EGKTYEETKP
FEPELTLNID AYIPDGYIDD EKQKIDMYKR FQAVDSQEDI HDLREELIDR FGDYPEEVEN
LFQVSVLKLY AKRERIESIT EKNKKVELIM EEEASQQIDG AKLFELANQY GRMIQLGTEG
KQLKVTFQFQ RDMYPRRYKV MEEVLQSLKD VKHDE
//
