ID A0A0A5FWJ6_9BACI Unreviewed; 794 AA.
AC A0A0A5FWJ6;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE SubName: Full=Beta-lactam antibiotic acylase {ECO:0000313|EMBL:KGX84299.1};
GN ORFNames=N784_13725 {ECO:0000313|EMBL:KGX84299.1};
OS Pontibacillus litoralis JSM 072002.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Pontibacillus.
OX NCBI_TaxID=1385512 {ECO:0000313|EMBL:KGX84299.1, ECO:0000313|Proteomes:UP000030401};
RN [1] {ECO:0000313|EMBL:KGX84299.1, ECO:0000313|Proteomes:UP000030401}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JSM 072002 {ECO:0000313|EMBL:KGX84299.1,
RC ECO:0000313|Proteomes:UP000030401};
RA Huang J., Wang G.;
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGX84299.1}.
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DR EMBL; AVPG01000042; KGX84299.1; -; Genomic_DNA.
DR RefSeq; WP_036836400.1; NZ_AVPG01000042.1.
DR AlphaFoldDB; A0A0A5FWJ6; -.
DR MEROPS; S45.003; -.
DR eggNOG; COG2366; Bacteria.
DR OrthoDB; 9759796at2; -.
DR Proteomes; UP000030401; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000030401};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT TRANSMEM 21..42
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT ACT_SITE 258
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 330
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 794 AA; 89575 MW; B455795284687BD0 CRC64;
MEVIPLPRRK KGWRTWQKVS LIVTLVLGFI VVGGLLFLNS WINRSLPQVE GEVAINGLHA
SVEVIRDANG VPHIKAENEW DLYRAQGFIQ AQDRLFQMDM ARRQASGQLS EVVGSATVTQ
DKYFRALGLR RAAQKSYEAY SQESKEILQA YADGVNAYIT QAEENNRLPV EFTLMGNSPE
PWTPLDSLTI GKYMAFDLGG HWERQAFHYY LLQTLPKEKA LELFSTYPEQ APTIIQEAEI
DVASLLQQAV IPHSYNGSNN WVVSGEKTAS GKPLLADDPH LGLATPSIWY EMHLSSPEQN
VSGVIFAGVP GIILGHNDSI AWGVTNTGPD VQQLYIEKRH PDQPNEFLFE GEYEEAAVIE
EPIKVKGEGT VAYEVMETRH GPIISQFAEA SGEEAVLSLR WTALDATAEL EAIIEMNKAT
NWNQFERALE KFEVPAQNFV FASQDGTIAY KANGKIPIYS NGDDALLPLE GWKAENEWDG
YIPWDELPRV VNPDKGYIAT ANNKVVGEDY PYHISNNWAQ PYRYKRIAQV LEQGDDFTAA
EMKNLQMDQH NLQGEEFVPI FLEHVDQGAL SDEEQKAIAI LKEWNYVDAV DGAAPLIFHT
WMQTFSKQLY EEDIPEEMIK LFNGRAQTTD ELIRQAHRGE EVIWIEEQGG LRQALTIALE
ETVAMLSEQY GSDATKWKWG DYHQVSFEHP LSSVSYLERF FNPKDPIPVG GSKVTVMAAA
YEEDTGIVDH GASWRFVADT ADLTQASHTV SPGQAGHFRS EWYHNQRDQW VTGEYHQTKV
KQYEGSVLML QPKE
//
