ID A0A0A5GNZ0_9BACI Unreviewed; 657 AA.
AC A0A0A5GNZ0;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Cyclic-di-AMP phosphodiesterase {ECO:0000256|PIRNR:PIRNR026583};
DE EC=3.1.4.- {ECO:0000256|PIRNR:PIRNR026583};
GN ORFNames=N781_00290 {ECO:0000313|EMBL:KGX93694.1};
OS Pontibacillus halophilus JSM 076056 = DSM 19796.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Pontibacillus.
OX NCBI_TaxID=1385510 {ECO:0000313|EMBL:KGX93694.1, ECO:0000313|Proteomes:UP000030528};
RN [1] {ECO:0000313|EMBL:KGX93694.1, ECO:0000313|Proteomes:UP000030528}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JSM 076056 {ECO:0000313|EMBL:KGX93694.1,
RC ECO:0000313|Proteomes:UP000030528};
RA Huang J., Wang G.;
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Has phosphodiesterase (PDE) activity against cyclic-di-AMP
CC (c-di-AMP). {ECO:0000256|PIRNR:PIRNR026583}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3',3'-c-di-AMP + H2O = 5'-O-phosphonoadenylyl-(3'->5')-
CC adenosine + H(+); Xref=Rhea:RHEA:54420, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:71500, ChEBI:CHEBI:138171;
CC Evidence={ECO:0000256|PIRNR:PIRNR026583};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the GdpP/PdeA phosphodiesterase family.
CC {ECO:0000256|PIRNR:PIRNR026583}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGX93694.1}.
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DR EMBL; AVPE01000001; KGX93694.1; -; Genomic_DNA.
DR RefSeq; WP_026799215.1; NZ_AVPE01000001.1.
DR AlphaFoldDB; A0A0A5GNZ0; -.
DR STRING; 1385510.GCA_000425205_00422; -.
DR eggNOG; COG3887; Bacteria.
DR OrthoDB; 9759476at2; -.
DR Proteomes; UP000030528; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0106409; F:cyclic-di-AMP phosphodiesterase activity; IEA:RHEA.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.310.30; -; 1.
DR Gene3D; 3.90.1640.10; inorganic pyrophosphatase (n-terminal core); 1.
DR Gene3D; 3.30.450.20; PAS domain; 1.
DR InterPro; IPR001667; DDH_dom.
DR InterPro; IPR038763; DHH_sf.
DR InterPro; IPR003156; DHHA1_dom.
DR InterPro; IPR049553; GdpP-like_PAS.
DR InterPro; IPR014528; GdpP/PdeA.
DR InterPro; IPR000160; GGDEF_dom.
DR PANTHER; PTHR47618; BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA; 1.
DR PANTHER; PTHR47618:SF2; CYCLIC-DI-AMP PHOSPHODIESTERASE GDPP; 1.
DR Pfam; PF01368; DHH; 1.
DR Pfam; PF02272; DHHA1; 1.
DR Pfam; PF21370; GdpP_PAS; 1.
DR PIRSF; PIRSF026583; YybT; 1.
DR SMART; SM00267; GGDEF; 1.
DR SUPFAM; SSF64182; DHH phosphoesterases; 1.
DR PROSITE; PS50887; GGDEF; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475,
KW ECO:0000256|PIRNR:PIRNR026583}; Hydrolase {ECO:0000256|PIRNR:PIRNR026583};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR026583};
KW Reference proteome {ECO:0000313|Proteomes:UP000030528};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..29
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 173..301
FT /note="GGDEF"
FT /evidence="ECO:0000259|PROSITE:PS50887"
SQ SEQUENCE 657 AA; 74376 MW; 2BDA449E6BE10146 CRC64;
MPNIFKKPTL SSHLWVIYLL SVLLLVVIYY YQWELGLIMT ALLAASIYYS VRSERHLLSE
TEEYISTLSH RVKKVGEEAL LEMPLGIVLY SEEHDIEWAN PYMNNFFPND TLVGKSLNEV
SKKLVPAIKE DKEEVWVEVE DYELQTQIKK EERLLYLFDR TKQKELQKSY QNDRTVLAII
FLDNYEEITQ AMDDTAKSHL NSQMTSLLNK WSQEHNIFLK RSSQEKFFAI MNQEILANLE
KYKFEILDEI RGLISENNVP LTLSFGIGSG EVPLPELGEL AQSSLDLALG RGGDQVVIKD
ENGKVRFYGG KTNPMEKRTR VRARVISHAL KELVRESDKV MIMGHKNPDM DSIGACIGIL
KVAQANDKDG FIVYDPDEMN TGVQRLIEEV EEHDELWSWF VSPDQAQEVV GKNTLLVVVD
THKPSLVIEE KLLSKTEHVV VIDHHRRAEE FIEDPTLVYM EPYASSTAEL VTELLEYQPK
QLKLNMLEST ALLAGIIVDT KSFTLRTGSR TFDAASYLRS KGADTVLVQK FMKEDLDVYV
NRSRLIERAT IYRDGIAISK GDHGETYGPV LIAQAADTLL TMSGVVCSFV ISEREDGRIG
ISARSLGDVN VQVIMESMNG GGHLTNAATQ LDDTSIEEAE AQLKEIIDEY VEGGTES
//
