ID A0A0A6NZ07_9GAMM Unreviewed; 427 AA.
AC A0A0A6NZ07;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Radical SAM domain-containing protein {ECO:0000313|EMBL:OAD21671.1};
DE Flags: Fragment;
GN ORFNames=THIOM_002554 {ECO:0000313|EMBL:OAD21671.1};
OS Candidatus Thiomargarita nelsonii.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Thiotrichales;
OC Thiotrichaceae; Thiomargarita.
OX NCBI_TaxID=1003181 {ECO:0000313|EMBL:OAD21671.1, ECO:0000313|Proteomes:UP000076962};
RN [1] {ECO:0000313|EMBL:OAD21671.1, ECO:0000313|Proteomes:UP000076962}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=THI036 {ECO:0000313|EMBL:OAD21671.1,
RC ECO:0000313|Proteomes:UP000076962};
RA Winkel M., Salman V., Woyke T., Schulz-Vogt H., Richter M., Flood B.,
RA Bailey J., Amann R., Mussmann M.;
RT "Single-cell genome of chain-forming Candidatus Thiomargarita nelsonii and
RT comparison to other large sulfur-oxidizing bacteria.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAD21671.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LUTY01001471; OAD21671.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0A6NZ07; -.
DR Proteomes; UP000076962; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 1..145
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 200..424
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT NON_TER 427
FT /evidence="ECO:0000313|EMBL:OAD21671.1"
SQ SEQUENCE 427 AA; 48794 MW; 3A4484AAA89DB2FF CRC64;
MKCLLISPPF DPNRITRRYP PVSLLYLAAS LRKFGHEPII YDLGVSSETS EDSLKELAFQ
SVKEHQPDLV GFTCSSPAFP LVRKSSAYIK EFFPNIKIIV GGMHPTLFPK EILENCSFID
YVAIGEGDNS LVKLCDMLDA NQTDFIAPGM AQKTREGKIV IGERLELIKN LDELPMPAWQ
DISIDDYRYD YSSWLNPKQH DISVVAPIST SRSCPFDCNF CAFNTLMGRG FRYHSPKRVV
DEIERLHTQF GVNYFEFIDD NMGIKKSRLI EICNEILKRN LDIQFTSMSG LHIATIDKDI
VDALCDAGYL HAILPIEHAS EFIRNKVIGK KLSHDKIFEV AELFKKRGTI TRAFFIIGFP
EETEETIEET MHMIKDLDLD LFNVFNLIPF PGTRLFQQCL EHKLFLNKVD TNTLWKGELA
LDTSDRS
//
