ID A0A0A6VTB1_9MICC Unreviewed; 1030 AA.
AC A0A0A6VTB1;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=GY22_11795 {ECO:0000313|EMBL:KHD97074.1};
OS Kocuria polaris.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Micrococcaceae;
OC Kocuria.
OX NCBI_TaxID=136273 {ECO:0000313|EMBL:KHD97074.1, ECO:0000313|Proteomes:UP000030466};
RN [1] {ECO:0000313|EMBL:KHD97074.1, ECO:0000313|Proteomes:UP000030466}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CMS 76or {ECO:0000313|EMBL:KHD97074.1,
RC ECO:0000313|Proteomes:UP000030466};
RX PubMed=12656171; DOI=10.1099/ijs.0.02336-0;
RA Reddy G.S., Prakash J.S., Prabahar V., Matsumoto G.I., Stackebrandt E.,
RA Shivaji S.;
RT "Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium
RT isolated from an Antarctic cyanobacterial mat sample.";
RL Int. J. Syst. Evol. Microbiol. 53:183-187(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DnaX/STICHEL family.
CC {ECO:0000256|ARBA:ARBA00006360}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KHD97074.1}.
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DR EMBL; JSUH01000010; KHD97074.1; -; Genomic_DNA.
DR RefSeq; WP_035927833.1; NZ_JSUH01000010.1.
DR AlphaFoldDB; A0A0A6VTB1; -.
DR OrthoDB; 9810148at2; -.
DR Proteomes; UP000030466; Unassembled WGS sequence.
DR GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR CDD; cd00009; AAA; 1.
DR CDD; cd18137; HLD_clamp_pol_III_gamma_tau; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 1.20.272.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR022754; DNA_pol_III_gamma-3.
DR InterPro; IPR012763; DNA_pol_III_sug/sutau_N.
DR InterPro; IPR045085; HLD_clamp_pol_III_gamma_tau.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR02397; dnaX_nterm; 1.
DR PANTHER; PTHR11669:SF0; PROTEIN STICHEL; 1.
DR PANTHER; PTHR11669; REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT; 1.
DR Pfam; PF13177; DNA_pol3_delta2; 1.
DR Pfam; PF12169; DNA_pol3_gamma3; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48019; post-AAA+ oligomerization domain-like; 1.
PE 3: Inferred from homology;
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022932};
KW Transferase {ECO:0000256|ARBA:ARBA00022932}.
FT DOMAIN 36..179
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 389..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 617..1000
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 493..517
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 656..679
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 767..786
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 848..867
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1030 AA; 107358 MW; A03B0FA615EA0110 CRC64;
MTTALYRRYR PETFEDVIGQ EHVTEPLMTA LRKDRVSHAY LFSGPRGCGK TTSARILARC
LNCAEGPTAT PCGRCDSCRD LARDGAGSLD VIEMDAASHG GVDHARDLRE RATFAPVRDR
YKIFIIDEAH MVTREGFNAL LKIVEEPPEH IKFIFATTEP SKVLTTIRSR THHYPFRLVP
TEPLMHYLEQ LCAEEHVQVA PGVLSLVVRA GAGSVRDTLS VLDQLMAGSD EQGIGYDLAV
ALLGYTHGAL LDDVVDAFAS GDGTTVFRAV DRVVQTGQDP RRFVEDLLER FRDLIIVNAV
PESAGNILHG MPQDQIARLR NQASQLGAAE LSRAADITNT ALTEMTGATS PQLHLELLCA
RILLPSSEDT TRGVTARVDR LERRLSIGRG AAAAGPDETP DAPAASPAAS SAASSSAPTA
PAGPGAPPGG RSHSQPASQS APAPERPAPP APGGDAQQAV PAVPRGTDPR ERTAQGAGQG
FGQEPGHAAA APERPSARED SGPERPTGRE APAAAERPDA PRQDAAAQAP RQDTPRAGEQ
GGNEIDLLRA SWPEIVNALA DIRRVAWFTV QRGTPQSFDG KVLRIMFDAE GDLMNFPRFE
DDLRQAVQQI LGIDCRAEAV PPGDGGHGRG GGGGGAPRGP EPSTRPPGRP AEPGPGTDGE
RPERRAPEDR SRGGQDRPGA PRGREQQPTP PRAASTPPDG APGTATDSAP EPPAGAPSPD
GDGGGQDPWD AHGRPATDAP AEWTDPAWAQ EPVHSWSVAA IPQGGAPAGP PAAPAPRAPA
QPEARPPGPA RTAEDPAGAQ PADRPAPQPG TQPGAPSDPG RTGRPGPAFP GEAPGARDRD
VPPSDDDVPP PEEPPYPDEP PYPDDLDAAP PEQWRPAAPA RQSPASAPRS GGGPGNRSTG
SATPPTAHPA ARPEDPRTAT GASAPAPTAN PAAPAAPAAP QQDAPSAAPA GADRNARLSF
RERHAAAIAA GRKAPASAAA PQDSGVEWDD SFVPSSDDEA LEDSTLYGRA AIERILGGML
IEERDLHTGE
//
