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Database: UniProt
Entry: A0A0A8K2W2_9RHIZ
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ID   A0A0A8K2W2_9RHIZ        Unreviewed;       633 AA.
AC   A0A0A8K2W2;
DT   04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT   04-MAR-2015, sequence version 1.
DT   26-FEB-2020, entry version 30.
DE   RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993443};
DE            EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993444};
GN   Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974};
GN   ORFNames=GL4_1389 {ECO:0000313|EMBL:BAQ16847.1};
OS   Methyloceanibacter caenitepidi.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methyloceanibacter.
OX   NCBI_TaxID=1384459 {ECO:0000313|EMBL:BAQ16847.1, ECO:0000313|Proteomes:UP000031643};
RN   [1] {ECO:0000313|EMBL:BAQ16847.1, ECO:0000313|Proteomes:UP000031643}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Gela4 {ECO:0000313|EMBL:BAQ16847.1,
RC   ECO:0000313|Proteomes:UP000031643};
RA   Takeuchi M., Susumu S., Kamagata Y., Oshima K., Hattori M., Iwasaki W.;
RT   "Genome sequencing of Methyloceanibacter caenitepidi Gela4.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC       molecules used as primers for DNA polymerase during DNA replication.
CC       {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
CC       ECO:0000256|SAAS:SAAS00709340}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|SAAS:SAAS00709317};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|PIRNR:PIRNR002811};
CC       Note=Binds 1 zinc ion per monomer. {ECO:0000256|PIRNR:PIRNR002811};
CC   -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC       Rule:MF_00974}.
CC   -!- SIMILARITY: Belongs to the DnaG primase family. {ECO:0000256|HAMAP-
CC       Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
CC       ECO:0000256|SAAS:SAAS00709351}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|HAMAP-Rule:MF_00974}.
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DR   EMBL; AP014648; BAQ16847.1; -; Genomic_DNA.
DR   RefSeq; WP_045365914.1; NZ_AP014648.1.
DR   EnsemblBacteria; BAQ16847; BAQ16847; GL4_1389.
DR   KEGG; mcg:GL4_1389; -.
DR   HOGENOM; CLU_013501_5_3_5; -.
DR   KO; K02316; -.
DR   OrthoDB; 1071997at2; -.
DR   Proteomes; UP000031643; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR   Gene3D; 3.90.580.10; -; 1.
DR   Gene3D; 3.90.980.10; -; 1.
DR   HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR   InterPro; IPR013264; DNA_primase_core_N.
DR   InterPro; IPR037068; DNA_primase_core_N_sf.
DR   InterPro; IPR019475; DNA_primase_DnaB-bd.
DR   InterPro; IPR006295; DNA_primase_DnaG.
DR   InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR   InterPro; IPR030846; DnaG_bac.
DR   InterPro; IPR034151; TOPRIM_DnaG_bac.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR002694; Znf_CHC2.
DR   Pfam; PF10410; DnaB_bind; 1.
DR   Pfam; PF13662; Toprim_4; 1.
DR   Pfam; PF08275; Toprim_N; 1.
DR   Pfam; PF01807; zf-CHC2; 1.
DR   PIRSF; PIRSF002811; DnaG; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SMART; SM00400; ZnF_CHCC; 1.
DR   TIGRFAMs; TIGR01391; dnaG; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993445};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709369};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709327};
KW   Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR002811,
KW   ECO:0000256|SAAS:SAAS00709338};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709339};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
KW   ECO:0000256|SAAS:SAAS00709304};
KW   Reference proteome {ECO:0000313|Proteomes:UP000031643};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709341};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993442};
KW   Zinc {ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709300};
KW   Zinc-finger {ECO:0000256|SAAS:SAAS00709301}.
FT   DOMAIN          257..339
FT                   /note="Toprim"
FT                   /evidence="ECO:0000259|PROSITE:PS50880"
FT   REGION          424..449
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        424..442
FT                   /note="Polyampholyte"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   633 AA;  70701 MW;  9114449C34686AEC CRC64;
     MKFSDSFLDE VRARLPVSQV VSRRVQLKRA GREWKGLSPF NKEKTPSFTV NDQKGFYHCF
     SSGRHGDIFR FVMETEGLSF PEAVERLAGE AGVPMPAPDP QYERTVKERL GLMDALEAAA
     KLFEQALRSG AGRDALTYAE GRGLSRETIA EFRIGYAPGG RDTLKNALLQ KGFTEAQLLD
     TGLVIKPEDG RPTYDRFRDR LTIPILDVKS RVIAFGARAL APDAQPKYLN SPETRLFDKG
     STVFNFARAR GSAFDKGELI VTEGYMDVIA LHQAGFKNAV ATLGTAFTAR QMEQLWQLAP
     EPVICFDGDR AGEAAAARAV DRMLPVLREG HSFRFAFLPQ GQDPDDLVRS AGASAFSAYL
     KNAMPLIDMA WRRELRAGAI DTPERRAAFE ARVEALLGEI ENVRVREHYR REIKNRLFEL
     WRPQPRSGKD GRPGRRNGAK PTRREALPPP TAYGFGTIVT LALVNHPWLL DRFAEEIASV
     DVTDKKLAAL LAAATKAIHD DHAITREALI AGLREGPHGA LLDRLLLESH HARHAFLKPD
     MPRDEVEEQF TDMLYRWRAL PTLTREIAES AEVIADMSEA EFERFAVLQQ EVASVGLKQD
     TDDAAMRDAK QRFQDTLARL KSAPPAKGRR RQG
//
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