ID   A0A0B0PIF8_GOSAR        Unreviewed;       593 AA.
AC   A0A0B0PIF8;
DT   04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT   04-MAR-2015, sequence version 1.
DT   24-JAN-2024, entry version 33.
DE   SubName: Full=Cucumisin {ECO:0000313|EMBL:KHG23166.1};
GN   ORFNames=F383_29751 {ECO:0000313|EMBL:KHG23166.1};
OS   Gossypium arboreum (Tree cotton) (Gossypium nanking).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX   NCBI_TaxID=29729 {ECO:0000313|EMBL:KHG23166.1, ECO:0000313|Proteomes:UP000032142};
RN   [1] {ECO:0000313|Proteomes:UP000032142}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. AKA8401 {ECO:0000313|Proteomes:UP000032142};
RA   Mudge J., Ramaraj T., Lindquist I.E., Bharti A.K., Sundararajan A.,
RA   Cameron C.T., Woodward J.E., May G.D., Brubaker C., Broadhvest J.,
RA   Wilkins T.A.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family.
CC       {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240}.
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DR   EMBL; KN424046; KHG23166.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0B0PIF8; -.
DR   Proteomes; UP000032142; Unassembled WGS sequence.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd02120; PA_subtilisin_like; 1.
DR   CDD; cd04852; Peptidases_S8_3; 1.
DR   Gene3D; 3.50.30.30; -; 1.
DR   Gene3D; 3.30.70.80; Peptidase S8 propeptide/proteinase inhibitor I9; 1.
DR   Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR034197; Peptidases_S8_3.
DR   InterPro; IPR010259; S8pro/Inhibitor_I9.
DR   InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR   InterPro; IPR045051; SBT.
DR   PANTHER; PTHR10795; PROPROTEIN CONVERTASE SUBTILISIN/KEXIN; 1.
DR   PANTHER; PTHR10795:SF706; SUBTILISIN-LIKE PROTEASE SBT4.3; 1.
DR   Pfam; PF05922; Inhibitor_I9; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; Subtilisin-like; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01240};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000032142};
KW   Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           26..593
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5002059387"
FT   DOMAIN          33..111
FT                   /note="Inhibitor I9"
FT                   /evidence="ECO:0000259|Pfam:PF05922"
FT   DOMAIN          133..580
FT                   /note="Peptidase S8/S53"
FT                   /evidence="ECO:0000259|Pfam:PF00082"
FT   ACT_SITE        141
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        196
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        529
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   593 AA;  63254 MW;  DE77053E722F7C8C CRC64;
     MAMSSFLLVV DITVILMLCI SHCHGAVEDD RKVYIAYLGA APEREDIASS QHSAMLQSLS
     TLSSSVENYL IKSYKRSFNG FAAKLTNEEA KKLASFKEVV SVFPSKVYHL QTTRSWDFLG
     FNQTVKRNAT AESNVIVGVL DTGIWPESDS FSDEGFGPRP KKWKGACKGG QNFTCNNKLI
     GTRAYHSDSA RDMEGHGTHT ASTAAGNNVV NASFYGLAEG IARGGVPSAR IAAYKVCSGI
     LCLSEDILAG FDDAIADGVD LISVSLGLEI PVDLYQDPLA IGAFHAAEKG VLVLQSAGNS
     GTTGFQSVSS VAAWILSVAA STTDHLFVDK VVLGNGWKTL TGFSVNSFSL NRTKVPLVYG
     LQVTSSCDEA HARACYSYCL NKTLVKNKIV LCDVMNGVNA AYDAGALGLI TKYQVENLSF
     VVPLSAITLS SEDYDLVISY HNSTKEPKAE ILRSETIKDK FAPIVASFFS RGPNAFVPDI
     LKARIGLITS PDISASGVDI LAAYSPAASP STTTTDPRRV KYNIISGTSM SCPHVAGVAA
     YVKTFHPHWS PSAIKSALMT TAFPMDAPRN QGAEFAYGSG HINPVKAIDP GLV
//