ID A0A0B1PD66_UNCNE Unreviewed; 2116 AA.
AC A0A0B1PD66;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 13-SEP-2023, entry version 36.
DE RecName: Full=glutamate synthase (NADH) {ECO:0000256|ARBA:ARBA00024383};
DE EC=1.4.1.14 {ECO:0000256|ARBA:ARBA00024383};
GN ORFNames=EV44_g4391 {ECO:0000313|EMBL:KHJ34609.1};
OS Uncinula necator (Grape powdery mildew).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Erysiphales; Erysiphaceae; Erysiphe.
OX NCBI_TaxID=52586 {ECO:0000313|EMBL:KHJ34609.1, ECO:0000313|Proteomes:UP000030854};
RN [1] {ECO:0000313|EMBL:KHJ34609.1, ECO:0000313|Proteomes:UP000030854}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=c {ECO:0000313|Proteomes:UP000030854};
RX PubMed=25487071;
RA Jones L., Riaz S., Morales-Cruz A., Amrine K.C., McGuire B., Gubler W.D.,
RA Walker M.A., Cantu D.;
RT "Adaptive genomic structural variation in the grape powdery mildew
RT pathogen, Erysiphe necator.";
RL BMC Genomics 15:1081-1081(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + L-glutamine +
CC NADH; Xref=Rhea:RHEA:13753, ChEBI:CHEBI:15378, ChEBI:CHEBI:16810,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:58359; EC=1.4.1.14;
CC Evidence={ECO:0000256|ARBA:ARBA00024267};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC Evidence={ECO:0000256|PIRSR:PIRSR000187-2};
CC Note=Binds 1 [3Fe-4S] cluster. {ECO:0000256|PIRSR:PIRSR000187-2};
CC -!- PATHWAY: Amino-acid biosynthesis; L-glutamate biosynthesis via GLT
CC pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+)
CC route): step 1/1. {ECO:0000256|ARBA:ARBA00004944}.
CC -!- PATHWAY: Energy metabolism; nitrogen metabolism.
CC {ECO:0000256|ARBA:ARBA00004802}.
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the glutamate synthase family.
CC {ECO:0000256|ARBA:ARBA00009716}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KHJ34609.1}.
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DR EMBL; JNVN01000790; KHJ34609.1; -; Genomic_DNA.
DR STRING; 52586.A0A0B1PD66; -.
DR HOGENOM; CLU_000422_8_2_1; -.
DR OMA; TVFRLQH; -.
DR OrthoDB; 20503at2759; -.
DR UniPathway; UPA00045; -.
DR UniPathway; UPA00634; UER00690.
DR Proteomes; UP000030854; Unassembled WGS sequence.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0016040; F:glutamate synthase (NADH) activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0097054; P:L-glutamate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00982; gltB_C; 1.
DR CDD; cd00713; GltS; 1.
DR CDD; cd02808; GltS_FMN; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 2.
DR Gene3D; 1.10.1060.10; Alpha-helical ferredoxin; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR Gene3D; 2.160.20.60; Glutamate synthase, alpha subunit, C-terminal domain; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR028261; DPD_II.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR002489; Glu_synth_asu_C.
DR InterPro; IPR036485; Glu_synth_asu_C_sf.
DR InterPro; IPR006982; Glu_synth_centr_N.
DR InterPro; IPR012220; Glu_synth_euk.
DR InterPro; IPR002932; Glu_synthdom.
DR InterPro; IPR006005; Glut_synth_ssu1.
DR InterPro; IPR009051; Helical_ferredxn.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR NCBIfam; TIGR01317; GOGAT_sm_gam; 1.
DR PANTHER; PTHR43100; GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN; 1.
DR PANTHER; PTHR43100:SF1; GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN; 1.
DR Pfam; PF14691; Fer4_20; 1.
DR Pfam; PF00310; GATase_2; 1.
DR Pfam; PF04898; Glu_syn_central; 1.
DR Pfam; PF01645; Glu_synthase; 1.
DR Pfam; PF01493; GXGXG; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR PIRSF; PIRSF000187; GOGAT; 1.
DR PRINTS; PR00419; ADXRDTASE.
DR SUPFAM; SSF69336; Alpha subunit of glutamate synthase, C-terminal domain; 1.
DR SUPFAM; SSF46548; alpha-helical ferredoxin; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
DR SUPFAM; SSF51971; Nucleotide-binding domain; 2.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
PE 3: Inferred from homology;
KW 3Fe-4S {ECO:0000256|ARBA:ARBA00023291, ECO:0000256|PIRSR:PIRSR000187-2};
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Glutamate biosynthesis {ECO:0000256|ARBA:ARBA00023164};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR000187-
KW 2}; Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000030854}.
FT DOMAIN 60..463
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000259|PROSITE:PS51278"
FT REGION 962..985
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 54
FT /note="For GATase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR000187-1"
FT BINDING 1186
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000256|PIRSR:PIRSR000187-2"
FT BINDING 1192
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000256|PIRSR:PIRSR000187-2"
FT BINDING 1197
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000256|PIRSR:PIRSR000187-2"
SQ SEQUENCE 2116 AA; 234153 MW; 9492A68FA467F1C8 CRC64;
MAYRDDFNDR QAQLDAEKLE YHEYVCLEPN SSSWAGALPS KQGLYDPELE KDACGVGFTC
NIKGVASHKI VSDARNLLCN MTHRGAVGSD ARDGDGAGVM TSIPHKFFIK NFERETNVKL
PLQGQYAVGN LFFKPDEETL QKSKIQLEKI AETLGLRVLG WRQVPKDSTL LGPAAASREP
VILQPFVVLR SAYDLSNSPQ DIDPVDFDER HFERQLFILR KRATHAVGLQ NWFYICSLSN
KNIVYKGQLS PSQVYDYFYD LVNADYEVHF ALVHSRFSTN TFPSWDRAQP LRWVAHNGEI
NTLRGNKNWM RAREGLMASE VFGDDLESLY PIIEEGGSDS AAFDNVLELL TINGVLSLPE
AIMLMVPEAW EGNALMDPAK TAFYEWAACL MEPWDGPALF TFSDGRYCGA NLDRNGLRPC
RFYITDDDRI ICASEVTTLN INPEKIIHKG RLQPGKMLLV DTLAGRIIDD GELKKTVSMR
YDFGSWVQKE LLLLPKIHEN LVNQTSMDLS SHLNELSIQE DPMLAAFGYS FEQVSLLLAP
MASDEKEALG SMGNDAPLAC LAKEPRLLYE YFRQLFAQVT NPPIDPIREA MVMSLSSYVG
PQGNLLKMDA SQCRRLSLRS PILSIPELNS LKNITKIHKD WTVKVIDLTF PKCEGTDGYI
KHLDEICRQA TAAIEHQDKI ILLSDRATCA DRLPVSALLA CGMVHHHLVT NRWRSLAALI
VETAEAREVH HMCVLLGYGA DAINPYLAME CIMKLNREGL IKKKLDDQTL IRNYKYSADG
GILKVMSKMG ISTLASYKGA QIFEALGIDD TVVNRCFKGT ATRIKGVTFD LIAADAFRFH
EKGFPSRQTI GIKGLIESGE YHWRDGGQPH INDPTSIANI QDAVRTKNDK SYEAYSISEY
EQIKSCTLRG MLDFKFDEST PVPINQVEPW TEIVRRFCTG AMSYGSISME AHSTLAIAMN
RLGGKSNTGE GGEDPERSER MSNGDTMRSA IKQVASGRFG VTSNYLADSD ELQIKMAQGA
KPGEGGELPG HKVSKSIART RHSTPGVGLI SPPPHHDIYS IEDLKQLIYD LKCSNPRARI
SVKLVSETGV GVVASGVAKA KADHILISGH DGGTGASRWT GIKYAGLPWE LGLAETHQTL
VLNDLRGRVI VQTDGQLRTG RDVAIACLLG AEEWGFATTP LIAMGCVFMR KCHLNTCPVG
IASQDPELRK KFQGTPEHVI NFFYYIANEL RAIMAKLGFR TINEMVGHAE RLRVRDDLRT
SKTENIDLSL ILTPAHKLRP GVATFNVRKQ DHRLYLRLDN KLISEAELTL DKGLPSRIEC
DIMNTDRAMG TSLSYQVSKR YGEEGLPIDT IHVNIKGSAG QSFGAFLAPG ITLELEGDAN
DYVGKGLSGG RLIIYPPRAA IFKAEENMLI GNVCLYGATS GTCYFRGIAA ERFAVRNSGA
TAVVEGVGDH GCEYMTGGRI LVLGSTGRNF AAGMSGGIAY ILDINQDFVT KLNHDMVEIS
GLDNPSEIAF VRGLIEDHHH YTGSELAARI LVDFNRALPR FVKVLPIDYK RVIAEEAAKA
AEAKRAAFEM PNLPGIQLRE KHDVKKSTSS ILDIEETVGD AATVKSKSLV LDKTRGFMRY
QRRSEKYRST KSRVRDWAEL SQRLDEDELK YQAARCMDCG VPFCQSDTGC PISNIIPKWN
ELVFQDQWRD ALNRLLMTNN FPEFTGRVCP APCEGACVLG INEDPVGIKS IECAIIDRGF
DMGWLVPNPP KIRTGKTVAI IGSGPAGLAC ADQLNKAGHL VTVYERSDRP GGLLMYGIPN
MKLDKKIVTR RTNLLAAEGI VFKTGITVGE DITLMKLKDE NDAVVIATGA TVARDLNIKN
RSLEGIHFAM EFLHANTKSL LDSGLTDGAY ISAKDKDVIV IGGGDTGNDC IGTSVRHGAK
SVTNFELLPQ PKSERTRDNP WPQWPRVYRV DYGHAEVKQH MGHDPREYCV MSQEFVDDGT
GKVKGINTHR VEWTKSATGG WDMTKVEGSE KFFPADLVLL SMGFLGPDAR ILDDKITKDG
RSNIKTPLGK YHTNVEGIFA AGDCRRGQSL IVWGINEGRQ SAREIDIWLE GCTRLPVAGG
IIKQTAQQVL HSIKVV
//