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Database: UniProt
Entry: A0A0B2A021_9MICO
LinkDB: A0A0B2A021_9MICO
Original site: A0A0B2A021_9MICO 
ID   A0A0B2A021_9MICO        Unreviewed;       588 AA.
AC   A0A0B2A021;
DT   04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT   04-MAR-2015, sequence version 1.
DT   10-OCT-2018, entry version 23.
DE   RecName: Full=Malto-oligosyltrehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
DE            Short=MTHase {ECO:0000256|PIRNR:PIRNR006337};
DE            EC=3.2.1.141 {ECO:0000256|PIRNR:PIRNR006337};
DE   AltName: Full=4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
DE   AltName: Full=Maltooligosyl trehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
GN   ORFNames=LK09_15135 {ECO:0000313|EMBL:KHK96331.1};
OS   Microbacterium mangrovi.
OC   Bacteria; Actinobacteria; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=1348253 {ECO:0000313|EMBL:KHK96331.1, ECO:0000313|Proteomes:UP000031030};
RN   [1] {ECO:0000313|EMBL:KHK96331.1, ECO:0000313|Proteomes:UP000031030}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MUSC 115 {ECO:0000313|EMBL:KHK96331.1,
RC   ECO:0000313|Proteomes:UP000031030};
RA   Lee L.-H.;
RT   "Genome sequence of Microbacterium mangrovi MUSC 115(T).";
RL   Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of (1->4)-alpha-D-glucosidic
CC       linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to
CC       yield trehalose and (1->4)-alpha-D-glucan.
CC       {ECO:0000256|PIRNR:PIRNR006337}.
CC   -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC       {ECO:0000256|PIRNR:PIRNR006337}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRSR:PIRSR006337-1}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|PIRNR:PIRNR006337, ECO:0000256|SAAS:SAAS00964676}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:KHK96331.1}.
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DR   EMBL; JTDK01000015; KHK96331.1; -; Genomic_DNA.
DR   RefSeq; WP_039401326.1; NZ_JTDK01000015.1.
DR   EnsemblBacteria; KHK96331; KHK96331; LK09_15135.
DR   UniPathway; UPA00299; -.
DR   Proteomes; UP000031030; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033942; F:4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005992; P:trehalose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR022567; DUF3459.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR012768; Trehalose_TreZ.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF11941; DUF3459; 1.
DR   PIRSF; PIRSF006337; Trehalose_TreZ; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   TIGRFAMs; TIGR02402; trehalose_TreZ; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000031030};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR006337};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR006337};
KW   Reference proteome {ECO:0000313|Proteomes:UP000031030}.
FT   DOMAIN      108    450       Aamy. {ECO:0000259|SMART:SM00642}.
FT   ACT_SITE    251    251       Nucleophile. {ECO:0000256|PIRSR:
FT                                PIRSR006337-1}.
FT   ACT_SITE    288    288       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR006337-1}.
FT   SITE        383    383       Transition state stabilizer.
FT                                {ECO:0000256|PIRSR:PIRSR006337-3}.
SQ   SEQUENCE   588 AA;  64236 MW;  38F1E52E07BD8C3C CRC64;
     MISVWAPRAE RVRVRLLDAG GRTRAEHDLE RMERDDGQTG WWASGLRLAD GDRYGFVLGD
     GDSLRPDPRS RRQPDGVHAA SAAFDAQAFA WTDAAWHGRQ LAGAVVYELH LGTFTPAGTL
     DAAIDRLDHL VALGVTHVEL LPVNAFNGAW NWGYDGVLWY AVHEGYGGPR AYQRFVDAAH
     ARGLAVVQDV VYNHLGPSGN YLPEFGPYLR EGSRNTWGDS IDLDQPPVRS FIVENALMWL
     RDHHVDGLRL DAVHALHDEQ PTHILKELAE QVDALSAHLG RPFTLIAESD LNDPTLILPR
     EAGGDGLTAQ WSDDWHHAVH VALTGETTGY YADFAAPDAL AKVTRGGFFH DGTYSSFRRR
     PHGTPIPDDV PAWRLVTFAQ DHDQIGNRAA GDRLSATLSP DRLAVAAVLT LTAPGTPMLF
     MGEEWGASTP WQFFTSHPEP ELGRATAEGR IAEFARMGWD PELVPDPQDP DTFARSHLDW
     GELAAAGHAR LLELYRALIA LRRDRPELTD PRNAANTATL VVDGDPDARA WRIERSGLAV
     LVNLTPHPVS FSVGDGRGIL LATREVTRSA GDVALPPDSA AVVAPPGP
//
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