ID A0A0B2RAX2_GLYSO Unreviewed; 944 AA.
AC A0A0B2RAX2;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN ORFNames=glysoja_034038 {ECO:0000313|EMBL:KHN30695.1};
OS Glycine soja (Wild soybean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3848 {ECO:0000313|EMBL:KHN30695.1};
RN [1] {ECO:0000313|EMBL:KHN30695.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Root {ECO:0000313|EMBL:KHN30695.1};
RA Lam H.-M., Qi X., Li M.-W., Liu X., Xie M., Ni M., Xu X.;
RT "Identification of a novel salt tolerance gene in wild soybean by whole-
RT genome sequencing.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR EMBL; KN651494; KHN30695.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0B2RAX2; -.
DR Proteomes; UP000053555; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944}.
FT DOMAIN 93..416
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 526..887
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
FT REGION 76..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 944 AA; 106079 MW; 0BFB199AA1CFE361 CRC64;
MVSGNIFHCR KNSWPPQEYI SKSTLQLFDY DSSAPPEQAW RRRLNSHANL LKEFRVRLGI
RIWSYVREEA SHGRKAPIDP FTRESCKPSA SQGVPLGGMG SGSISRGFRG EFRQWQIIPS
LCEASPVMSN QFSIFISREG GKKKFASVLA PGQHEGLGSS RKPDDQGISS WGWNLSGQHS
TYHALFPRAW TVYDGEPDPE LKISCRQISP FIPHNYRESS LPAAVFVYTL VNTGKERAKV
SLLFTWANSI GGSSHSSGDH VNEPFKAEDG VSGVLLYHKQ TAKGNPPVTF AIAACETQNV
NVSVLPSFGL SEESSMTAKH MWSKMVKDGQ FDQENFNSGP SMPSSPGETL CAAVAASTWV
EPHGKCTVAF SLAWSSPKVK FVKGSTFNRR YTKFYGTSEK AAADLAHDAL THYNRWEEEI
EKWQNPVLKD EALPEWYKFT LFNELYFLVA GGTIWIDSPV LSSNMRNDQD RVRELESAVV
KETEDKMSDR KRTVVESTTD STYDSAVITG HDRADEKLYE DDDDVGRFLY LEGVEYIMWC
TYDVHFYASF ALLELFPRIE LNIQRDFARA VLCEDGRKVK FLAEGNWGIR KVYGAVPHDL
GTHDPWHEMN AYNIHDTSKW KDLNPKFVLQ VYRDFATTGD LQFGVDVWPA VRAAMEYMEQ
FDRDGDGLIE NDGFPDQTYD TWTVHGVSTY CGCLWLAALQ AAAVMALELG DREFAETCKR
KFLKAKPAFE EKLWNGTYFN YDSGSSGNSK SIQADQLAGQ WYTASSGLPS LFEDSKIKSA
LRKVYDFNVM KVKGGRMGAV NGMHPNGKVD ETCMQSREVW TGVTYGLAAT MIHAGMEEEA
FTTAEGIFLA GWSEDGYGYW FQTPEAWTMD GHYRSLMYMR PLAIWGMQYA INRPKAILEA
PKINIMDRIH LSPVIGGYSH NETGVRKIAT KAGCFSNSVF NCAC
//
