ID A0A0B2V8B6_TOXCA Unreviewed; 1004 AA.
AC A0A0B2V8B6;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=NOL1/NOP2/Sun domain family member 4 {ECO:0000256|ARBA:ARBA00042050};
GN Name=nsun4 {ECO:0000313|EMBL:KHN77768.1};
GN ORFNames=Tcan_14499 {ECO:0000313|EMBL:KHN77768.1};
OS Toxocara canis (Canine roundworm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Spirurina; Ascaridomorpha; Ascaridoidea; Toxocaridae; Toxocara.
OX NCBI_TaxID=6265 {ECO:0000313|EMBL:KHN77768.1, ECO:0000313|Proteomes:UP000031036};
RN [1] {ECO:0000313|EMBL:KHN77768.1, ECO:0000313|Proteomes:UP000031036}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PN_DK_2014 {ECO:0000313|EMBL:KHN77768.1};
RA Zhu X.-Q., Korhonen P.K., Cai H., Young N.D., Nejsum P.,
RA von Samson-Himmelstjerna G., Boag P.R., Tan P., Li Q., Min J., Yang Y.,
RA Wang X., Fang X., Hall R.S., Hofmann A., Sternberg P.W., Jex A.R.,
RA Gasser R.B.;
RT "Genetic blueprint of the zoonotic pathogen Toxocara canis.";
RL Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a cytidine in rRNA + S-adenosyl-L-methionine = a 5-
CC methylcytidine in rRNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:61484, Rhea:RHEA-COMP:15836, Rhea:RHEA-COMP:15837,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74483, ChEBI:CHEBI:82748;
CC Evidence={ECO:0000256|ARBA:ARBA00036484};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a cytidine in tRNA + S-adenosyl-L-methionine = a 5-
CC methylcytidine in tRNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:61468, Rhea:RHEA-COMP:13670, Rhea:RHEA-COMP:15827,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74483, ChEBI:CHEBI:82748;
CC Evidence={ECO:0000256|ARBA:ARBA00036266};
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KHN77768.1}.
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DR EMBL; JPKZ01002233; KHN77768.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0B2V8B6; -.
DR STRING; 6265.A0A0B2V8B6; -.
DR Proteomes; UP000031036; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd00200; WD40; 1.
DR Gene3D; 6.20.240.40; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR011044; Quino_amine_DH_bsu.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR46955:SF6; 5-METHYLCYTOSINE RRNA METHYLTRANSFERASE NSUN-4; 1.
DR PANTHER; PTHR46955; PROTEIN CBG01349-RELATED; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR02008; RCMTFAMILY.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR SUPFAM; SSF50969; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 3.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW Reference proteome {ECO:0000313|Proteomes:UP000031036};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; WD repeat {ECO:0000256|PROSITE-ProRule:PRU00221}.
FT REPEAT 131..172
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 173..214
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 215..256
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT DOMAIN 695..1003
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 928
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 798..804
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 821
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 854
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 873
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 1004 AA; 110119 MW; 94B42C5470BD978F CRC64;
MRAFGRDRWL NYKATESSFL SFGGIEKEAH AERMSGEGSL ADDKYLTENA VVVQHDWFSC
VRSDQTDSSF FVGIREYGSP SSFIDVRHKG GKLFPATPDK VHVEKLDARR LAVQFKNMAP
TTFVAPRIEF TTVHSTSVQS VDVSPSGRLV VSSDSNGQLV VWHADTAEII RNFVGHALDV
NRCRFFPSGL VVLSAGMDMS VRIWSVETGQ CPRVLKGHRQ GVVDLGIIDR GKEILSCSKD
GTAKMWNCGS AECLRTWCPE AGLGATISID KEEGTSVAFG TSEGANLYIG TAGGSVISYD
MKAGKTFAKL KTSRGAVTKL CVRESLGLFS SFCVVDLGII DRGKEILSCS KDGTAKMWNC
GSAECLRTWC PEAGLVNAIA VTPSSSGVFS VACEMGKAEV YDIRSSEPGA TISIDKEEGT
SVAFGTSEGA NLYIGTAGGS VISYDMKAGK TFAKLKTSRG AVTKLCVRES LGLFSSFYDG
TVSCYDLPLN SKMAKLEFSG ADCDPVYDMS TAAEGQLYTA CRDRIMLRRI CSSCSSTVSS
TSSTCSKLCS PPNLCKERVR HKRLKFKPKV ARTRPMKTPS ALAIEHFDFY YGPMYGKRWP
SIRLGLLTPN KYVAVVNTFS KAWETNEYIL QDMGAVDVIK TLTGTTATER IEKKRKLIEA
KGIRTPDAER DEHEQANSDE MEDLAMRGEA GLNEFRRSGQ MLTRGELQMG QKPDSRDQHD
ITITGLEGQY VQLPKKEDFI VYPRKLKLYS MPRGSLVDFP SPIKDEQGIP SWWLLDGGSI
IPVLALDLSE GETLLDMCAA PGGKSLLAIQ TGLPEKMVCN DYKLSRLGQL RRALSMFIPL
DSDAADRVIL KRKDASDLQR WDELAVYDKV LVDAPCSTDR LAANQDEGNM FAVSMTTERL
NLPELQAKLL INALRSVRVG GSVVYSTCTL SPIQNETVVE NAVAVATERF GLKVIEQTLE
QLERHLSSSG LFRFGESCRR GSLVVPFLPS NFGPMYVCKL RRTL
//
