ID A0A0B2WRF1_METAS Unreviewed; 2536 AA.
AC A0A0B2WRF1;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=D-lysergyl-peptide-synthetase subunit 1 {ECO:0000256|ARBA:ARBA00044125};
GN ORFNames=MAM_06182 {ECO:0000313|EMBL:KHN96077.1};
OS Metarhizium album (strain ARSEF 1941).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX NCBI_TaxID=1081103 {ECO:0000313|EMBL:KHN96077.1, ECO:0000313|Proteomes:UP000030816};
RN [1] {ECO:0000313|EMBL:KHN96077.1, ECO:0000313|Proteomes:UP000030816}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ARSEF 1941 {ECO:0000313|EMBL:KHN96077.1,
RC ECO:0000313|Proteomes:UP000030816};
RX PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA St Leger R.J., Wang C.;
RT "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT adaptation.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
CC -!- PATHWAY: Alkaloid biosynthesis; ergot alkaloid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005107}.
CC -!- SIMILARITY: Belongs to the NRP synthetase family.
CC {ECO:0000256|ARBA:ARBA00029454}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KHN96077.1}.
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DR EMBL; AZHE01000018; KHN96077.1; -; Genomic_DNA.
DR STRING; 1081103.A0A0B2WRF1; -.
DR HOGENOM; CLU_000022_60_2_1; -.
DR OrthoDB; 2549913at2759; -.
DR Proteomes; UP000030816; Unassembled WGS sequence.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR CDD; cd05918; A_NRPS_SidN3_like; 2.
DR CDD; cd19545; FUM14_C_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 3.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 3.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 1.
DR Pfam; PF00668; Condensation; 3.
DR Pfam; PF00550; PP-binding; 2.
DR SMART; SM00823; PKS_PP; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 6.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000030816}.
FT DOMAIN 532..608
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1609..1685
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 2513..2536
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2536 AA; 278697 MW; 80341D4EB41585A5 CRC64;
MQILPPTLRE CHTIINHFLA NVSRWRDGRD AICSAETCLT YAQLDDYSIK FARYLIQIGF
QKGDIILCRH GKSPWAIVSF LGILRAGLAF TPVDPSYPAA RVQQIANQTN SRLILELFSY
QEVSTSLESL QLDAGFFRSL VDNASIAVPD VQGTDLAYVF FTSGSTGEPK GVMVDHKAIC
SSLLAHGQRL GMHQGTRALQ ATSYTFDPCL TEIFATLIYG GCVCVPTGWA ELAESINRLR
VNWAFFTPSV ISLLAPEDVP SLTTVAVGGE ALTQDCVDTW SSRVKLFNSY GPTEACVFSC
ISSVSPDSSP SNIGYASGCH VWVVDAENHN RICAVGEVGE LVIQGDILAR GYLGNAQKTA
EVFVRCEGAS NTENLPGRIY KTGDLAKLEP DGSISCLGRK DRQVKIQGQR VELRDIESHI
LQHSRDKIQT VVVEKENKNN SDYLVAFLIL GSYRWAARAK ASWDTWLGDL KSHLSRSLPS
YMVPDHFVPM ETLPKTSSGK ADRAVLLEIF SRAVSKNGQR HARDTKDEQS CEGSGPALVS
IKMEVARVLS LDFCHLDSSS NFFQLGGNSL LAIKLVARLR RSGIQLPLKA VLDGESLATL
ATSRASRLRH GSLATSTSQP FHDLGEGFAT PRGLGELGRW AADQCGLPPH AIESILPCTA
FQESLLPSFN REPCAYVTQY VFKLPPNLDL DRFKSCWAHA YNQLGVLRTR ILLPPNRGPI
QVIVDKQITW DLHDELSQSS CHISRAKLGL GGELSNFALY TTGTGRCFVL TLHHVLFDEW
SLQLLLDHVA QLYDGKQQAI NPGVSFDSYV HFSIRASRNP ASKAFWQKHL DGFSSPSFPT
IPRPDYQPEL RDSLTITEHC SASALLIEGS LALALGTLTG QRDVCFGTVM SGRDAGMPGI
DEVLGPTVST LPFRIAWDCH QSISEFVAAL RTRRDLVRPH EQLGIHNIAK VCPGAANACD
FQTMLVIQAP SPRDKSYALF QDREETENRD SHSLVLECTM TPSSLVIDAR FDPSVVSAVR
CRRLLHSWTH MLRQMLEAES DARLASLSWI SGEDLATLGR WNESLPPPVR GLIHDILPHA
AQKHPRKTAV ESWDGSLTYS ELDDVSSRLA AELVAWNPTK IIALHFPKGI SMVVSMWAVL
KAGRAFLPLD IESPGDRVTS IITQLESPLV LSHDSTALKR LLPVDCWTVN KAFLSALPVL
PEKWSPPEMS GHDLAYVIMT SGSTGRPKGV MIEHQSVMTH LTSSAARYSI DGSTRILQFA
SVAFDMCVYE VTVSALHGAC LCVPPQGVDA DTWGDFVRHK NVNWSFFTPS FLRSIKPANF
PSFKTIIVGG EAVTLDAVES WAPRVNLLNV YGPTECTITC MNGRIRSDCS NSSSIGKAMG
SASWIVDPED HNRLMPLGAV GELLIEGFVV GRGYMNDADK TAAAFILPPS WASPGQFGRD
FARMYKTGDL ACYDDTGSLV FVGRKDNQIK LRGQRLELAE VEHHVEKAAK GRQSLCFVPH
GGPMARRLIA VISAGRRNEL GSQSSPSIVA MRPDRAIRAD IQSIRNRVSK AVPGYMVPSF
VAVIREIPIA LTGKADRKAV GSWIQNLSDT EALFFSTGED VASDLMKEEP TNNIESALQS
VWAEVLNISA DKIGMHQSFQ SVGGDSITAM QAVARCRNRG ICITMKSLLQ ASGIRTIGAE
ALDGPVDPRT SSSLVEGHEQ RFPLSPLQKA YVALAPQNQV HHFNQTCQVM LRHAVDEKSL
QAAMRLVVKQ HASLRTRYNL STPEASTQRI ADAIDGSFST SSTCMSDIES LNRLVLAIQS
IINPVSGPII HCELVSGPED ACRLILVATH LAVDIVSWRL ILEDLEAILE GKSLRPVPPG
LFRQWCKRRR VQEQTKLSLP VPNYNYWAID PSHLTYMQAR RESVSLSTGA TSLCLGSSNN
CLRTKPTDLF IAALLLAFCE AFPDRDSPAV CLEGHGRRQA PGDPDISGTV GWFTAVTPVY
APYKTGQEAT DFARKIKDAR FWAERNHADF FGDQQILDGH YPRIPEIMLN FTGVDQEGDF
VSGGMFTADP DSCGDDYDFS GNMRRFAIFD VAASVRRGRL RFEFLHNQGM SKSARVKTWI
SAFLETIEGL AAKLAVTSPL LTQSDYPNAS LTYAQLQCLE LHLGSHNIAT VACNGDTRPR
ATSVFAATPI QEQILHSQQV NPYCWRPTVI LAVTTRDGSP VNMQDLLAAW RQVITANPIL
RTIFVPKPCP QQKFAAEEFL NVVLDEACTM SSTVKVLSGT ENLLVLPPTK WRPGEPQHRL
TILPRVARHA VLCRWEVNHA LMDHGSMPSV LAAFSRAYAG EPSVNGATCA YQSYATQLDR
LASAAGVEFW QTYLQRALPS RLTGQAPPNR SVYAARSTFA TPVDLTLANR ATARALETAN
TTTATVFRLA WARVVATEVD RDDVVLGFVV SGRDAQIDDI DRVVGPTFNI MPCRVTNIHR
RSTKELLQCL RDDFLAATGH QHSLARYLKK AKQRGGPLFD SVVNFRRHVQ ASPDDPGALV
IEEQPGSQDP YELQWS
//
