ID A0A0B3BZ25_9PSED Unreviewed; 1147 AA.
AC A0A0B3BZ25;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=PT85_01720 {ECO:0000313|EMBL:KHO66316.1};
OS Pseudomonas flexibilis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=706570 {ECO:0000313|EMBL:KHO66316.1, ECO:0000313|Proteomes:UP000030980};
RN [1] {ECO:0000313|EMBL:KHO66316.1, ECO:0000313|Proteomes:UP000030980}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 14085 {ECO:0000313|EMBL:KHO66316.1,
RC ECO:0000313|Proteomes:UP000030980};
RA Shin S.-K., Yi H.;
RT "Genome sequence of Pseudomonas tuomuerensis JCM 14085.";
RL Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KHO66316.1}.
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DR EMBL; JTAK01000001; KHO66316.1; -; Genomic_DNA.
DR RefSeq; WP_039605756.1; NZ_JTAK01000001.1.
DR AlphaFoldDB; A0A0B3BZ25; -.
DR STRING; 706570.PT85_01720; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000030980; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000030980}.
FT DOMAIN 615..776
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 797..951
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1147 AA; 129031 MW; 9C889354A84A5C0B CRC64;
MSVLRLPTLP SAAGKQHWGN LPGAALSLAI AEAASAAGRR LTLLLTPDSQ SAERLKEELA
FFAPTLPVLH FPDWETLPYD QFSPHQDIIS QRIATLYRLP EIRHGILVVP VNTALHRLAP
TRFLLGRSLV LDVGQRLDVE DMRGRLEAAG YRCVETVYEH GEFAVRGALL DLFPMGSDTP
YRIDLFDDEI ETLRTFDPES QRSVDKVESI RLLPGREFPL DKKSVTDFRG RFREHFDVDF
RRCPIYQDLS TGITPAGIEY YLPLFFEETS TLFDYLPEDT QVFSLPGIEQ AAEQFWLDVR
NRHEDRRLDP ERPVLPPAEL FVPVEDCFAR LKRWPRVVVD SQDLQTGVGR ERFPARPLPD
LALQAKAAEP LAALRRFLEE YSGRVLFSAE TAGRREALLE LLGRLKLKPR EVNGWVDFLA
SGERLNICIA PLDEGLLLDD LALIPESPLF GQRVMQRRRR EKTRDHGDNV IKHLTELREG
APVVHIDHGV GRYQGLVTLD YDGQSNEFLV LQYADDAKLY VPVTSLHLIA RYTGSDEALA
PLHKLGSEQW QKAKRKAAEQ VRDVAAELLD IYARRAARKG YAFKDPAADY ETFSAAFPFE
ETVDQEAAIE AVRHDMLASK PMDRLVCGDV GFGKTEVAMR AAFIAVHSGK QVAVLVPTTL
LAEQHYNSFR DRFADWPVRV EVMSRFKSAK EIADAAALLA EGKVDIVIGT HRLLQNDIRF
KNLGLLIIDE EHRFGVRQKE QLKALRSEVD ILTLTATPIP RTLNMAVAGM RDLSIIATPP
ARRLSVRTFV MERQPAVIKE ALLRELLRGG QVYFLHNDVK TIEKCAAELA ALVPEARIAI
GHGQMPERQL EQVMSDFYHK RFNVLVASTI IETGIDVPTA NTIIIERADK FGLAQLHQLR
GRVGRSHHQA YAYLLTPPRR QMTEDAQMRL EAIANAQDLG AGFVLATHDL EIRGAGELLG
EGQSGQIQAV GFTLYMEMLE RAVKAIQKGE QPNLDQPLAS GTEINLRVPA LIPDDYLPDV
HGRLILYKRI ASAPDEDALQ ELQVEMIDRF GLLPEPAKHL IRQTLLKLQA EKLGIQKIDA
GPQGGRLEFS ANTCVDPLTL IKLIQSQPNR YKFEGATLFK FQVPMERPEE RFNTLEALLE
RLAPQPA
//
