ID A0A0B5EP74_STRA4 Unreviewed; 4120 AA.
AC A0A0B5EP74;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE SubName: Full=Type I polyketide synthase {ECO:0000313|EMBL:AJE81080.1};
GN ORFNames=SLNWT_0704 {ECO:0000313|EMBL:AJE81080.1};
OS Streptomyces albus (strain ATCC 21838 / DSM 41398 / FERM P-419 / JCM 4703 /
OS NBRC 107858).
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1081613 {ECO:0000313|EMBL:AJE81080.1, ECO:0000313|Proteomes:UP000031523};
RN [1] {ECO:0000313|EMBL:AJE81080.1, ECO:0000313|Proteomes:UP000031523}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 21838 / DSM 41398 / FERM P-419 / JCM 4703 / NBRC 107858
RC {ECO:0000313|Proteomes:UP000031523};
RA Lu C.;
RT "Enhanced salinomycin production by adjusting the supply of polyketide
RT extender units in Streptomyce albus DSM 41398.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; CP010519; AJE81080.1; -; Genomic_DNA.
DR KEGG; sals:SLNWT_0704; -.
DR Proteomes; UP000031523; Chromosome.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR CDD; cd08952; KR_1_SDR_x; 2.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 6.10.140.1830; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR041618; PKS_DE.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF18369; PKS_DE; 2.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 4.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000031523};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..423
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 904..979
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 995..1422
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2430..2505
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2528..2955
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3965..4040
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 874..901
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4120 AA; 429583 MW; ADF041D58AF0DFBB CRC64;
MAVVGLACRL PGAEDPAAFW DLLVRGGDAV VDTPEDRRAG APAAGYPAPH GGTAPHRGGY
LRAPADFDAA FFGISPREAD AMDPQARLVL ELGWEALEHA GIPATRAPRT TAVYIGSDVC
DYADLARRHG SEAVGHHTLT GLQRALIANR LSYALGVSGP SVTVDSAQSS SLVSVQMACD
SLRLGDSALA LAGGVHLNLS PDSTRAAAQF GALSPDGRCH TFDARANGYV RGEGGALIVL
KLLSAAVADG DRVLAVIRGG AVNNDGTGRS LTTPDAAAQQ EVLRAACARA GVDPAHLDYV
ELHGTGTRAG DPVEATALGA VLGRADGRAT PLAVGSVKTN IGHLEGAAGA AGLLKTVLCL
AHGTLVPSLH HREPHPAVDL AGLNLRVQTA IGPWTPAAPD RPLLAGVSSF GMGGTNAHLV
LAQAPAAEQA PRAPREAPAT VPWLLSAHTP QAFKAQAERL ADHVTRHPAD PVDTAWSLLE
TRAHLPHRAV VVGTDPEDLV RGLATALPAG PVRGDIDRPV FVFPGQGSQW VGMAGQLLAE
APAFATRLAE CAEALAPHVD WDLMAVLRGD PDAPSLDRSD VVQPALWAVM VSLAAQWQAH
GVRPAAVVGH SQGEIAAACV AGALGLADAA RVVALRSKVL VPLEGLSGMV SLALTEADTR
RFTARWGERV TLAALNGPSS TVVSADADTV LDILATAERE QIRARRVGID YASHSPHVEP
AREEMLRQLA PLTPRTPQVP FLSTVTGDWI QGPDTDAGYW YDNLRKPVLL EPVVRTLTEA
GHGAFLEVSP HPVLTTPVQD TVETAAGRES AVVTGTLRRE DGGPARFQTS LAALWARGAD
VDWTPFFTGL APRRVALPTY AFQRRRHWLD DAPAAPTAAS AEPAAAPARQ ADPTPGPVTD
TPVENPLDLV RAHAAVVLGH HDATEIDPQL SFKELGFDSV TAVELRDRLA SATGRSLPAT
AVYAYPTPAR LAHHLGAGED ESAPEPTGAG AADADDPVVI VSMGCRYPGG VGSPEELWHL
VREEADALSP FPTDRGWDLD GLFDTDPDTS GTTYVRSGGF LDTATEFDAA FFGISPREAL
AMDPQQRLLL ETVWETLERA GLDPRSLAQS PTGVFVGATA AEYGPRLHEA TEDVEGHLLT
GTTASVASGR ISYALGLEGP AVTVDTACSS SLVALHLAVR ALRAGECSLA LAAGATVMSS
PGMFVEFSRQ RGLAADGRCK PFAAEADGTG WAEGVGVLLL ERLSDARANG HQVLAVVRGS
AINQDGASNG LSAPSGPAQQ RVIRAALADA GVPASGVDVV EAHGTGTKLG DPIEAQALLA
TYGREHDAEN PLWLGSVKSN IGHTQAAAGV AGVIKTVQAM RAGTLPKSLH ADRPSPIVDW
SSGGVALLGS ARPWDTEDGR PRRAAVSSFG ISGTNAHVVL EQPPALAEDA SATEPAAPAA
ELPWLLSARN ADALAAQHDR LREYVAAHEP RPVDVAWSLV AGRSVFEHRA VVMGGEVVAS
GAAGASGANA SNGAKPVFVF PGQGAQWVGM AVELLDSSPV FAERFGECAA ALEEFVEWSL
FDVVRGVEGA PGFDRVDVVQ PALWAVMVSL AALWESYGVR PAAVVGHSQG EIAAACVAGA
LSLTDGARVV ALRSRALVSL AGRGGMVSVA VDRARAEELA GRWPGRVSVA ALNGPSSTVV
SGDADALDEL VGQADELGVR ARRIEVDYAS HSAHVDAIEA ELAELLAPVV AREPEVPFFS
TVTGEWVNSG ETDASYWFRN LRGRVLLEPV VRALVEEGHG VFVEVSPHPV LAVPVGETVE
ALGVEGAVVG SLRRDQGGLE RMYASLGEAW AHGVEVDWSP VFAGQSPQRV DLPTYAFQRQ
RYWLESAKTA ADVAVDPVEA EFWASVERND LDALGRTLGV EDAASLGEVV PLLSSWRRER
LDRSTLDGWR YRIAWRELAV PAAGELNGTW LLVTPAEEEG DDALTAGLRE ALAARADRVV
VLPVPVRDGD RARYAALLSA AGEPGEFAGV LSTLGLREEP HPAQASVPLG LAAGLALVQA
LGDTGIEAPL WFLTRGAVGT GQGDAPTAAL QAGVWGLGRV AALEHPGRWG GLVDLPADAG
AETFARLTGI LAGCGGEDQL ALRDGKVLGR RLVPAPTGGR APARDWSPRG TVLVTGGTGA
LGGHIARWLA RNGAEHLVLT GRRGDAPHLD ALRADLEPYG TRLTVAGCDT SDRDAVAELV
ARLDAEGTPV RAVLHAAGVS VLGPLAEVRV EDLAATFSGK VLGADHLDEV LAGREVDAVV
YFSSISGTWG VADHAAYGSA NAVLDARAEQ RRAEGSPVLS VAWGPWAGGG MIAESVQEDL
RRRGVPVIAP ETAVVGLQQA LDHDESYVAV ADVDWGRFTG VFTSVRRSPL LDELPGARPA
VPERRTPEAA EGHAPALREI AALDPARRTA ALHTLVAGQV AAALGHQDAG ALSGEAAFKE
LGFDSLTAVE LRNRLGRTLG LTLPTTVVFD HPSIVALTAF VQEQAFGREE SAAESARPSV
RAAAGEADDP VVIVSMGCRY PGGVGSPEEL WRLVREEADT AAPFPTDRGW DLDGLFDADP
DHLGTSYVRE SSFLKDPAGF DAGFFGISPR EALAMDPQQR LLLETTWETL ERGGIDPKRL
RGSSTGVYVG LTDQEYASLL RKSSGENEGY LATGAAASVA SGRISYALGL EGPAVTVDTA
CSSSLVALHL AVRALRAGEC TLAVAAAATV MSGPSPFVAF SRQRALAPDG RCKPFAAGAD
GFALAEGVGV VLLERLSDAR ANGHRVLAVV RGSAVNQDGA SNGLSAPNGP SQQRVIRAAL
ADAGVPASGV DVVEAHGTGT KLGDPIEAQA LLATYGRERD PEHPLWLGSV KSNIGHTQAA
SGMAGIIKMV QAMAARTLPK SLCAEEPSPF VDWSGGAVEL LDSTREWAEP EGRPRRAAVS
SFGISGTNAH VILEAPEEAP APAPEEAADL PGAEIPLLVS AREEAALTAQ TRRILDLSRT
GDVRPVDVAW SLVAGRSAFE HRAVLLGGGV VASGVAAASG ANGSKPVFVF PGQGAQWAGM
AVELLDSSPV FAERFGECAA ALDEFVEWSL FDVVRGAEGA PGFDRVDVVQ PALWAVMVSL
AALWESYGVR PAAVVGHSQG EIAAACVAGA LSLVDGARVV ALRSRALSPL AGRGGMVSVA
LGSGAVEDLV GRWGTRLSVA AVNGPSSTVV SGDVGALDEL MGVAESLGVR TRRIEVDYAS
HSAHVDAIEA ELAQLLAPVV AREPEVPFFS TVTGEWVNSA ETDAGYWFRN LRSRVLLEPV
VRALVEEGHG VFVEVSPHPV LAVPVGETVE AAGVEGAVVG SLRRGQGGLE RMYGSLGEAW
AHGVEVDWSP VFAGISPRRV DLPTYAFQRQ RYWLESAKTA ADVAVDPVEA EFWASVERND
LDALGRTLGV EDAASLGEVV PLLSSWRRER LDRSTLDGWR YRIAWRAHRP SDSAQLNGSW
LLITPVSGAD ENLVESVRQT MEKAGAEVDV LALTGPADRA ALVSRLRPRD AEPAAGVLSL
LAFDETSDPG SPAVSRGLVD SLVLAQALTE AGGGRLWALT RGAVTVDGTA APDPVQAAVR
GMLRVAGLDD PERCGGLIDL PADGVGEDLL AAVPALLAAE GTQGRETEYA VRAEGVHVRR
MVRAPLDPSA DGGPWQPRGT VLVTGGTGAL GGHVARDLAR SGAEHLLLAS RSGAKAPGAP
ELEQELTALG CKVTLASCDI ADRAAVEELV RTVPAEFPLT AVVHTAGAVD LARPLTELDT
GTAVALMHAK VAGARNLHEV LAGRPLDAFV LFSSGAGVWG NTGQAPYAAA NAYLDALAEL
RRAEGLPATA IAWGAWAGGG MVDTEVGENL ARRGVPAMPP QLAVRAVRES AADPRGALVV
ADIRWDRFLT AYSVHGHRPL LDEVPDVQAL LAERAAEEAA EEAATGTGNE LLRELAALPQ
DKRRRRLTEV IRAHAAAVLT LGPGESVKAG RAFRDMGFDS LTAVDLRNRL GTSLGAKLSA
TLVFDHPTPK ALADHLAEEL LPEEAGPGDG TDPRLEGVAD AYRKASGPAE RQELADALRA
LLDEWAHPTD EPEQAAVDEE LVDASDQDMF ELIDRELGIS
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