ID A0A0B8MZ32_9NOCA Unreviewed; 823 AA.
AC A0A0B8MZ32;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN Name=uvrD {ECO:0000313|EMBL:APA95564.1};
GN ORFNames=NS07_v2contig00001-0043 {ECO:0000313|EMBL:GAM44194.1},
GN NS506_01493 {ECO:0000313|EMBL:APA95564.1};
OS Nocardia seriolae.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=37332 {ECO:0000313|EMBL:GAM44194.1, ECO:0000313|Proteomes:UP000019401};
RN [1] {ECO:0000313|EMBL:GAM44194.1, ECO:0000313|Proteomes:UP000019401}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N-2927 {ECO:0000313|EMBL:GAM44194.1,
RC ECO:0000313|Proteomes:UP000019401};
RA Imajoh M., Fukumoto Y., Yamane J., Sukeda M., Shimizu M., Ohnishi K.,
RA Oshima S.;
RT "Draft Genome Sequence of Nocardia seriolae Strain N-2927 (NBRC 110360),
RT Isolated as the Causal Agent of Nocardiosis of Yellowtail (Seriola
RT quinqueradiata) in Kochi Prefecture, Japan.";
RL Genome Announc. 3:e00082-15(2015).
RN [2] {ECO:0000313|EMBL:APA95564.1, ECO:0000313|Proteomes:UP000180166}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EM150506 {ECO:0000313|EMBL:APA95564.1,
RC ECO:0000313|Proteomes:UP000180166};
RA Han H.-J.;
RT "Genome sequence of Nocardia seriolae strain EM150506, isolated from
RT Anguila japonica.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
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DR EMBL; CP017839; APA95564.1; -; Genomic_DNA.
DR EMBL; BAWD02000001; GAM44194.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0B8MZ32; -.
DR STRING; 37332.NS506_01493; -.
DR KEGG; nsr:NS506_01493; -.
DR PATRIC; fig|37332.12.peg.1522; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000019401; Unassembled WGS sequence.
DR Proteomes; UP000180166; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000019401}.
FT DOMAIN 24..325
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 326..630
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 717..764
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 722..751
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 823 AA; 91293 MW; AAEFAF3DF8867B75 CRC64;
MNTTVARDEQ KMVEQARGGG ALLEGLNPQQ RAAVMHTGTP LLIVAGAGSG KTAVLTRRIA
YLLDARGVRP GEILAITFTN KAAAEMRERV AGLVGPRAAG MWVSTFHSSC VRILRTQAGL
VAGLNSNFSI YDADDSRRLL GMIIRDFDID AKKYSPRLLS TAISNLKNEL IDPEQAAADA
ETEDAELPRI VAKVYDEYQR RLRAANAMDF DDLIGETVSL LQRHPQVAEY YRRRFRHVLV
DEYQDTNHAQ YVLVRELVGH HRNLWGDATE TDDDNRVPPS ELCVVGDADQ SIYAFRGATI
RNIEEFERDF PDAETILLEQ NYRSTQHILT AANAVIARNE GRREKKLWTD AGDGDLIVGY
VADNEHDEAS FVAREIDKLV DQGDANYADV AVFYRTNNNS RALEEIFIRM GLPYKVVGGV
RFYERKEVRD IVAYLRVLEN PDDAVSLRRV LNTPRRGIGD RAEACVAVHA EQRNIGFAQA
LRDAAEGKVA LLNTRSQRAI SGFLELLEEI RAAGKQEDMD FPDVGSVVEA VLDRTGYRAE
LEASDDPQDG ARLDNLNELV SVAREFSSEA RNNVEAARAE GILPEQAEGE PEPGSLAAFL
ERVSLVADAD QIPEEGSGVV TLMTLHTAKG LEFPVVFVTG WEDGQFPHMR ALGDPTELQE
ERRLAYVGIT RARKRLYLTR AIVRSGWGQP VSNPESRFLQ EIPGHLLDWR RLEPAPSQAS
RGFRRRGEDD GLERDWTRGD GWSEQRPGVR GERRPAPAAA ASGKRNNTNL VLAVGDRVND
DKYGLGRVIA AEGFGALATV TIDFGTAGKI RLIPQYSRTL TKL
//
