ID A0A0C1F594_9FLAO Unreviewed; 943 AA.
AC A0A0C1F594;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=OA86_11695 {ECO:0000313|EMBL:KIA88377.1};
OS Kaistella jeonii.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC Chryseobacterium group; Kaistella.
OX NCBI_TaxID=266749 {ECO:0000313|EMBL:KIA88377.1, ECO:0000313|Proteomes:UP000031473};
RN [1] {ECO:0000313|EMBL:KIA88377.1, ECO:0000313|Proteomes:UP000031473}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17048 {ECO:0000313|EMBL:KIA88377.1,
RC ECO:0000313|Proteomes:UP000031473};
RA Clayton J.T., Newman J.D.;
RT "Kaistella jeonii genome.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIA88377.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JSYL01000008; KIA88377.1; -; Genomic_DNA.
DR RefSeq; WP_039353386.1; NZ_LR134503.1.
DR AlphaFoldDB; A0A0C1F594; -.
DR STRING; 266749.SAMN05421876_1105; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000031473; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 7..268
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 352..532
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 701..907
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
FT COILED 556..590
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 943 AA; 107233 MW; 7E4A906CA9E2F3F6 CRC64;
MSENNDKRLF LIDAYAMIFR GYYALIRSPR ITSTGIDTSA IFGFTNSLIE LIRRERPTHL
AVVFDVGQAS VRTADYADYK ANRSETPEAI KLAIPYIHRI LKAMHVPILG LEGYEADDVI
GTIACKAEKE NYTVFMVTPD KDFAQLVTPN IKMYKPGLKG AEFEILGVDE IKAKYEIEDP
KQVIDFLAMM GDAVDNIPGL EGVGEKTAKK FLKEYGSIEK LLENTADLKG KLKEKVENSA
ERGILSKKLA TIICDAPIEF HQEQYDLDDP DFEKVKEVFD EIEFRRLYEN LYRAFAPAAT
VASTSNKNSA EEKVQKPVIV NQTMQLDLFA NFEDLDQATT TKETVSDSDH LYQYIYSPKA
QKVLVHNLLQ QKAVSFDTEI TSLDEMEADL IGMSFSYKKG LAYYIPFSEK REEVLETLEI
FRPFFEKKEI VKIAHNLKTD YKILKQYGIE VEGAIFDTMI AHYLLNPDGR HAMEYLSEMY
LGYKPVAIES LIGKGKNQIS FKELSVEEQT KFAAEDADIS WRLYEVFAPQ LKIENLEDLF
YKVEMPLMKV LAKMELEGIS LDKKWLEQES KDLENDLRQL EKTIFELSGE EFNMNSPKQL
GEVLFEKMQL DPKAKKTKSG QYATSEDILQ KLSSKHEIIK HILEYRTYQK LKSTYVDALP
LQIDKDDHRV HTNFSQTTAA TGRLASVNPN LQNIPIRTLR GQQIRGAFVA NPGNKLIAAD
YSQIELRLIA AISEEDNMIK AFQDGEDIHA STASKLFNIP LEEVTKTQRS QAKTVNFGIL
YGQGAFGLAE QTGLSRKEAK EMIESYFETY PKLKKYMADQ VIKAQEMGYV ETILNRKRHL
KDINSANFVV KAHAERNAVN APIQGSAADV IKLAMIKIDE QFTEQNLKTK MLLQVHDELV
FEAPEEELET VTALIKNEME SAYKTTVPLI VEVGVGDNWL EAH
//
