ID A0A0C1FVM0_9SPHI Unreviewed; 626 AA.
AC A0A0C1FVM0;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:KIA91909.1};
GN ORFNames=OC25_19180 {ECO:0000313|EMBL:KIA91909.1};
OS Pedobacter kyungheensis.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Pedobacter.
OX NCBI_TaxID=1069985 {ECO:0000313|EMBL:KIA91909.1, ECO:0000313|Proteomes:UP000031246};
RN [1] {ECO:0000313|EMBL:KIA91909.1, ECO:0000313|Proteomes:UP000031246}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KACC 16221 {ECO:0000313|EMBL:KIA91909.1,
RC ECO:0000313|Proteomes:UP000031246};
RA Anderson B.M., Newman J.D.;
RT "Pedobacter Kyungheensis.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIA91909.1}.
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DR EMBL; JSYN01000025; KIA91909.1; -; Genomic_DNA.
DR RefSeq; WP_039479432.1; NZ_JSYN01000025.1.
DR AlphaFoldDB; A0A0C1FVM0; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000031246; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:InterPro.
DR CDD; cd11350; AmyAc_4; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43002:SF10; 1,4-ALPHA-GLUCAN BRANCHING ENZYME GLGB; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..626
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002132085"
FT DOMAIN 188..539
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 336
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
FT ACT_SITE 382
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
SQ SEQUENCE 626 AA; 70213 MW; DD9B54D41A342AC2 CRC64;
MKKVFYLLLL VAATHTACKK AATDTPEPTP VNQTADLPAG AKNGVAFINN GTSAIVTLYA
PGKTSVSVIG DFNNWSTTAS VMKKTPDGNT WWVQIDNLNP ATEYAYQFVV DGNLKVADPY
CEKVLDPDND QYIAATVYPN LKAYPTGKTT GIVSTMQANQ PVYNWKNTSF TRPDKNNLVI
YELLVRDFTT EHSYASTLAK LDYLIGLGIN AIELMPVNEF EGNLSWGYNP SFYFAPDKYY
GTKTALQNFI DECHGRGIAV IMDMVLNHSF GQSPMVQLYF DGAKPTTSSP WFNVDAKHPF
NVGYDFNHES QATKKFSKDV MKFWMQQYKI DGFRFDLSKG FTQKSSTDDA QFRLYDAGRV
AIWKDYNSYI KSIDPNFYVI LEHFAEESEE KVLADEGMML WNNLNHDMNE ATMGWLGSSN
FQWGFYTNHG FTKSENLVSY GESHDEERLN FKNISYGNGF GSYMIKGNLA TSLKREELIA
AFLFGMPGPK MIWQFGELGY DVSIDFNGRT GDKPIKWEYF SDPNRKALYD AYAKFIRLKK
NNSIFNSTNF TYNLAGGIKY IKLTEGSNTV VVVGNFDVIN QSANIDFGTA GTWLDAVGSN
VSLASGTYTA NLAPGEYHIF SKVALK
//
