ID A0A0C1G0A1_9SPHI Unreviewed; 481 AA.
AC A0A0C1G0A1;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=Probable membrane transporter protein {ECO:0000256|RuleBase:RU363041};
GN ORFNames=OC25_13655 {ECO:0000313|EMBL:KIA93499.1};
OS Pedobacter kyungheensis.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Pedobacter.
OX NCBI_TaxID=1069985 {ECO:0000313|EMBL:KIA93499.1, ECO:0000313|Proteomes:UP000031246};
RN [1] {ECO:0000313|EMBL:KIA93499.1, ECO:0000313|Proteomes:UP000031246}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KACC 16221 {ECO:0000313|EMBL:KIA93499.1,
RC ECO:0000313|Proteomes:UP000031246};
RA Anderson B.M., Newman J.D.;
RT "Pedobacter Kyungheensis.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin;
CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76;
CC Evidence={ECO:0000256|ARBA:ARBA00001156};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
CC {ECO:0000256|ARBA:ARBA00005010}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU363041};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU363041}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the 4-toluene sulfonate uptake permease (TSUP)
CC (TC 2.A.102) family. {ECO:0000256|RuleBase:RU363041}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIA93499.1}.
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DR EMBL; JSYN01000015; KIA93499.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0C1G0A1; -.
DR UniPathway; UPA00262; UER00222.
DR Proteomes; UP000031246; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004325; F:ferrochelatase activity; IEA:InterPro.
DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR028161; Met8-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR028281; Sirohaem_synthase_central.
DR InterPro; IPR006367; Sirohaem_synthase_N.
DR InterPro; IPR002781; TM_pro_TauE-like.
DR NCBIfam; TIGR01470; cysG_Nterm; 1.
DR PANTHER; PTHR35330; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR PANTHER; PTHR35330:SF1; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR Pfam; PF14824; Sirohm_synth_M; 1.
DR Pfam; PF01925; TauE; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU363041};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU363041};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU363041};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU363041}.
FT TRANSMEM 198..216
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 232..256
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 333..352
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 364..392
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 398..420
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 427..445
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 457..478
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT DOMAIN 121..144
FT /note="Siroheme synthase central"
FT /evidence="ECO:0000259|Pfam:PF14824"
SQ SEQUENCE 481 AA; 52252 MW; C8B04819DC27D7A7 CRC64;
MFPVFVKLNK LRTLLIGAGN IGLEKLTAIV NNSHQANITI VAEVVSPEVY TLIANYPLVK
VKQKSFDVSD LQDIDIVFAA TNNNILNEEI RRVTNEKGLL INVADKPELC DFYLGSIVQK
GDLKIAISTN GKSPTIAKRL KQILNEGLPA ELDETLQNMS ALRQTLNGDF SAKVKKLNKV
TQSLINPKKS FAERNIKWLI WVASVLLIVA IGFSLWNTEP EFQAFLINID PLFYWFLGAG
FVFALIDGAI GMSYGVTTAS FSLAMGLPPA SASMAIHISE VMSNGIAGWM HYRMGNVNWK
LFKILILPAI VGAVLGAYIL SSLEHYSAYV KPIVAVYTLF LGGIILMKAF NIKRKKADQK
IKKIGLLGFG GGFIDAAFGG GWGSIVLSSL IAGGRHPLFS LGTVKISRFF IATLSSLTFF
TMLGGKHWEA VLGLIIGSAI ASPIAAKVSN KISAKTIMVA VGIIVMLVSL RSVIMFILKL
I
//
