ID A0A0C1R8L1_9ACTN Unreviewed; 553 AA.
AC A0A0C1R8L1;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 13-SEP-2023, entry version 37.
DE RecName: Full=Choline dehydrogenase {ECO:0000256|RuleBase:RU003969};
DE EC=1.1.99.1 {ECO:0000256|RuleBase:RU003969};
GN ORFNames=MB54_03070 {ECO:0000313|EMBL:KIE49922.1};
OS marine actinobacterium MedAcidi-G2B.
OC Bacteria; Actinomycetota; Actinomycetes.
OX NCBI_TaxID=1550401 {ECO:0000313|EMBL:KIE49922.1, ECO:0000313|Proteomes:UP000031352};
RN [1] {ECO:0000313|EMBL:KIE49922.1, ECO:0000313|Proteomes:UP000031352}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mizuno C.M., Rodriguez-Valera F., Ghai R.;
RT "Novel reconstructed genomes of marine planktonic Acidimicrobiales.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=A + choline = AH2 + betaine aldehyde; Xref=Rhea:RHEA:17433,
CC ChEBI:CHEBI:13193, ChEBI:CHEBI:15354, ChEBI:CHEBI:15710,
CC ChEBI:CHEBI:17499; EC=1.1.99.1;
CC Evidence={ECO:0000256|RuleBase:RU003969};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRSR:PIRSR000137-2};
CC -!- PATHWAY: Amine and polyamine biosynthesis; betaine biosynthesis via
CC choline pathway; betaine aldehyde from choline (cytochrome c reductase
CC route): step 1/1. {ECO:0000256|RuleBase:RU003969}.
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC {ECO:0000256|ARBA:ARBA00010790, ECO:0000256|RuleBase:RU003968}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIE49922.1}.
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DR EMBL; JUEO01000003; KIE49922.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0C1R8L1; -.
DR STRING; 1550401.MB54_03070; -.
DR UniPathway; UPA00529; UER00385.
DR Proteomes; UP000031352; Unassembled WGS sequence.
DR GO; GO:0008812; F:choline dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0019285; P:glycine betaine biosynthetic process from choline; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR Gene3D; 3.30.560.10; Glucose Oxidase, domain 3; 1.
DR InterPro; IPR011533; BetA.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR012132; GMC_OxRdtase.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR NCBIfam; TIGR01810; betA; 1.
DR PANTHER; PTHR11552:SF147; CHOLINE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR11552; GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS00623; GMC_OXRED_1; 1.
DR PROSITE; PS00624; GMC_OXRED_2; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRSR:PIRSR000137-2};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU003968};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:KIE49922.1}.
FT DOMAIN 85..108
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS00623"
FT DOMAIN 258..272
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS00624"
FT BINDING 87
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 224
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
SQ SEQUENCE 553 AA; 61644 MW; 324D4897A1077DC5 CRC64;
MLTKTYDYVV IGGGSAGSAL ANRLSADPKN KVLLIEAGRS DWKIDPIIHM PAALSMGIGN
RLYDWKYESE PEPQMNGRRV YHARGKVLGG SSSINGMIFQ RGNPMDFDRW AAIEGCEDWD
WSHCLPYFKR METCLAGPDE WRGGEGPLKL ERGPATSPLF QAFFTAVQEA GHPLTTDVNG
YRQEGFNAFD RNIYRGRRHS AARAYLHPVL RDRKNLHLIT RAHVTQLIMK KNRVTGVEYH
KNKRRHRVEA TEVISCGGAF NSPQLLQLSG IGPSSVLSQA GISPVVDLPG VGENLQDHLE
VYVQYACKEP VSMAPYLKNW RKPGVGLKWL FRKGPGATNH FEAGGFLRSN DGVLWPNLMF
HFLPVAVRYD GTTITQGHGY QFHIGPMFSN STGWVRIQSN DPFAKPKIQF NYLSTPEDRK
EWVEAIATAR NIMKQPAFEQ FNGGEISPGP EISEEKDVLD WVRRDSETAL HPCGTARMGE
DAMGVVSPKT MRVHGVEGLR VVDASVMPTL TNGNIYAPVM MLAEKAADLI LGNTPLEPSR
AGQYRHGVSE KDW
//
