ID A0A0C2EG74_9PSED Unreviewed; 236 AA.
AC A0A0C2EG74;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Dual-specificity RNA pseudouridine synthase RluF {ECO:0000256|ARBA:ARBA00039989};
DE EC=5.4.99.21 {ECO:0000256|ARBA:ARBA00038922};
DE AltName: Full=23S rRNA pseudouridine(2604) synthase {ECO:0000256|ARBA:ARBA00041697};
DE AltName: Full=Ribosomal large subunit pseudouridine synthase F {ECO:0000256|ARBA:ARBA00042890};
DE AltName: Full=rRNA pseudouridylate synthase F {ECO:0000256|ARBA:ARBA00042843};
DE AltName: Full=rRNA-uridine isomerase F {ECO:0000256|ARBA:ARBA00043147};
DE AltName: Full=tRNA(Tyr) pseudouridine(35) synthase {ECO:0000256|ARBA:ARBA00041420};
GN ORFNames=UCMB321_1367 {ECO:0000313|EMBL:KIH85039.1};
OS Pseudomonas batumici.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=226910 {ECO:0000313|EMBL:KIH85039.1, ECO:0000313|Proteomes:UP000031535};
RN [1] {ECO:0000313|EMBL:KIH85039.1, ECO:0000313|Proteomes:UP000031535}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCM B-321 {ECO:0000313|EMBL:KIH85039.1,
RC ECO:0000313|Proteomes:UP000031535};
RA Klochko V.V., Zelena L.B., Elena K.A., Reva O.N.;
RT "Complete genome of Pseudomonas batumici UCM B-321 producer of the batumin
RT antibiotic with strong antistaphilococcal and potential anticancer
RT activity.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2604) in 23S rRNA = pseudouridine(2604) in 23S rRNA;
CC Xref=Rhea:RHEA:38875, Rhea:RHEA-COMP:10093, Rhea:RHEA-COMP:10094,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.21;
CC Evidence={ECO:0000256|ARBA:ARBA00036535};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(35) in tRNA(Tyr) = pseudouridine(35) in tRNA(Tyr);
CC Xref=Rhea:RHEA:60556, Rhea:RHEA-COMP:15607, Rhea:RHEA-COMP:15608,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|ARBA:ARBA00036390};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIH85039.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JXDG01000012; KIH85039.1; -; Genomic_DNA.
DR RefSeq; WP_040064815.1; NZ_JXDG01000012.1.
DR AlphaFoldDB; A0A0C2EG74; -.
DR STRING; 226910.UCMB321_1367; -.
DR PATRIC; fig|226910.6.peg.1358; -.
DR OrthoDB; 9807213at2; -.
DR Proteomes; UP000031535; Unassembled WGS sequence.
DR GO; GO:0140098; F:catalytic activity, acting on RNA; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProt.
DR CDD; cd02555; PSSA_1; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR PANTHER; PTHR47683:SF2; S4 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS50889; S4; 1.
PE 4: Predicted;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 5..65
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
SQ SEQUENCE 236 AA; 26124 MW; 4ED90872C6535B50 CRC64;
MSEPIRLSKR LIELVGCSRR EAELFIEGGW VTVDGEVIDE PQYKVAAQKV ELDPEAKATA
PEPVTLLLHQ PAGLDLDSAL QLLRADTLSE EHRYGKRPLK GHFLRLTAGA DLQAGASGLV
VFTQDWKILR KLTADAAKIE QEYVVEVSGD IVEHGLNRLS HGLTYKGREL PAVKASWQNE
NRLRFAMKNP QPGVIALFCQ AVGLKVVAIR RIRIGGVSIG KVPVGQWRYL SAKEKF
//
