ID   A0A0C2I8K8_9PSED        Unreviewed;       378 AA.
AC   A0A0C2I8K8;
DT   01-APR-2015, integrated into UniProtKB/TrEMBL.
DT   01-APR-2015, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   RecName: Full=Nickel/cobalt efflux system {ECO:0000256|RuleBase:RU362101};
GN   ORFNames=UCMB321_3006 {ECO:0000313|EMBL:KIH83260.1};
OS   Pseudomonas batumici.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=226910 {ECO:0000313|EMBL:KIH83260.1, ECO:0000313|Proteomes:UP000031535};
RN   [1] {ECO:0000313|EMBL:KIH83260.1, ECO:0000313|Proteomes:UP000031535}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCM B-321 {ECO:0000313|EMBL:KIH83260.1,
RC   ECO:0000313|Proteomes:UP000031535};
RA   Klochko V.V., Zelena L.B., Elena K.A., Reva O.N.;
RT   "Complete genome of Pseudomonas batumici UCM B-321 producer of the batumin
RT   antibiotic with strong antistaphilococcal and potential anticancer
RT   activity.";
RL   Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Efflux system for nickel and cobalt.
CC       {ECO:0000256|ARBA:ARBA00002510}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU362101};
CC       Multi-pass membrane protein {ECO:0000256|RuleBase:RU362101}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC       {ECO:0000256|RuleBase:RU362101}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KIH83260.1}.
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DR   EMBL; JXDG01000039; KIH83260.1; -; Genomic_DNA.
DR   RefSeq; WP_040068157.1; NZ_JXDG01000039.1.
DR   AlphaFoldDB; A0A0C2I8K8; -.
DR   STRING; 226910.UCMB321_3006; -.
DR   PATRIC; fig|226910.6.peg.2995; -.
DR   OrthoDB; 271709at2; -.
DR   Proteomes; UP000031535; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   PANTHER; PTHR40659; NICKEL/COBALT EFFLUX SYSTEM RCNA; 1.
DR   PANTHER; PTHR40659:SF1; NICKEL_COBALT EFFLUX SYSTEM RCNA; 1.
DR   Pfam; PF03824; NicO; 2.
PE   3: Inferred from homology;
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW   Cobalt transport {ECO:0000256|ARBA:ARBA00022426};
KW   Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU362101};
KW   Nickel {ECO:0000256|ARBA:ARBA00022596};
KW   Nickel transport {ECO:0000256|ARBA:ARBA00023112};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW   ECO:0000256|RuleBase:RU362101};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|RuleBase:RU362101};
KW   Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU362101}.
FT   TRANSMEM        15..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        54..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        91..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        279..300
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        312..334
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        355..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
SQ   SEQUENCE   378 AA;  41409 MW;  9382497F937F373A CRC64;
     MPNLAELLQQ GGSHAWLYFP SAILLGALHG LEPGHSKTMM AAFIIAIRGT LKQAVLLGLA
     ATLSHTAVVW LVAIGGMYLG KGLDAQTTEP YFQLASSALI IVIALWMLWR TWNGERMARF
     EQDDHHHHGE HDHGHPDETH RIDTGHGRIE LSIFEEGMPP HWRFKTLTGH AWSASDVRLT
     TTRPDGSTQS FAFIEREGFL ESTVDIPEPH EFSARLSLGH AGHSHDYDLE YQEHDHGHVH
     SELEGLELSI DGYQDAHERA HANDIRKRFT NRKVTTGQIV MFGLTGGLIP CPAAITVLLL
     CLQVKEVALG GMLVLCFSIG LAITLVTVGA AAAIGARQAS NRWPWLGSVA RRAPYLSSVL
     IIGVGLYVGI HGWIGLNA
//