ID A0A0C2RDA5_9BACL Unreviewed; 620 AA.
AC A0A0C2RDA5;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=5,10-methylenetetrahydrofolate reductase {ECO:0000313|EMBL:KIL48260.1};
GN ORFNames=KP78_17070 {ECO:0000313|EMBL:KIL48260.1};
OS Jeotgalibacillus soli.
OC Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae; Jeotgalibacillus.
OX NCBI_TaxID=889306 {ECO:0000313|EMBL:KIL48260.1, ECO:0000313|Proteomes:UP000031938};
RN [1] {ECO:0000313|EMBL:KIL48260.1, ECO:0000313|Proteomes:UP000031938}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P9 {ECO:0000313|EMBL:KIL48260.1,
RC ECO:0000313|Proteomes:UP000031938};
RA Goh K.M., Chan K.-G., Yaakop A.S., Ee R., Gan H.M., Chan C.S.;
RT "Genome sequencing of Jeotgalibacillus soli.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU00333};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIL48260.1}.
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DR EMBL; JXRP01000013; KIL48260.1; -; Genomic_DNA.
DR RefSeq; WP_041087872.1; NZ_JXRP01000013.1.
DR AlphaFoldDB; A0A0C2RDA5; -.
DR STRING; 889306.KP78_17070; -.
DR PATRIC; fig|889306.3.peg.1715; -.
DR OrthoDB; 9803687at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000031938; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00537; MTHFR; 1.
DR Gene3D; 3.20.20.220; -; 1.
DR Gene3D; 3.20.20.330; Homocysteine-binding-like domain; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR003726; HCY_dom.
DR InterPro; IPR036589; HCY_dom_sf.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR PANTHER; PTHR11103:SF18; HOMOCYSTEINE S-METHYLTRANSFERASE 1-RELATED; 1.
DR PANTHER; PTHR11103; SLR1189 PROTEIN; 1.
DR Pfam; PF02219; MTHFR; 1.
DR Pfam; PF02574; S-methyl_trans; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
DR SUPFAM; SSF82282; Homocysteine S-methyltransferase; 1.
DR PROSITE; PS50970; HCY; 1.
PE 4: Predicted;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Metal-binding {ECO:0000256|PROSITE-ProRule:PRU00333};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU00333}; Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000031938};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU00333}; Zinc {ECO:0000256|PROSITE-ProRule:PRU00333}.
FT DOMAIN 1..281
FT /note="Hcy-binding"
FT /evidence="ECO:0000259|PROSITE:PS50970"
FT BINDING 201
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00333"
FT BINDING 266
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00333"
FT BINDING 267
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00333"
SQ SEQUENCE 620 AA; 68335 MW; F929858D39D4FC4A CRC64;
MKLIDALQER ILIADGAMGT LLYSYGTDQC FEELNLSAAE HIQQIHTAYI QAGANVIQTN
TYAGNYHKLE RYGLEDYVKE INRAGVKNAR QAALADTFVV GTIGGIRSLN PQTVKLEEIK
RSFREQLYCL LLEGVDGILL ETYYDLEELS TVVSIARKET DIPIIAQASL QEVGVLQDGT
PITEAFKQLE DLGADLVGLN CRLGPYHMLR TLEGVPLPKH AFLSSYPNAS LPSYSDGALQ
YNNNAEYFRE VAHSFQTQGV RLLGGCCGTT PDHIRAFSEA LKGAVPVLEK DVYIQQPKIE
VSSRRIYVPE PLQNIVTKRS SIIVELDPPK KLNIDAFMKG AKALKEAGAD AVTLADNSLA
SPRVSNESLG HLIKAEHDVR PLVHITCRDR NLIGLQSHLM GLHTLGLHDV LAVTGDPTKV
GDFPGATSVY DVSSFELIEM IQQLNRGVSM SGKDLGESTS FSIGAAFNPN VRSVERAVLR
MEKKIKAGAD YFMSQPVFSE EKLLEVYEYV KDINAPLYIG LMPLTNSRNA DFLHHEVPGI
KLSDQVRERM ATVAGDKERS SQEGLAIAKS LIDTALELFN GIYLITPFMR YDLTVELTKY
AKQQAAKRAG GVNHVSNIIH
//
