ID A0A0C2RMP5_9BACL Unreviewed; 1543 AA.
AC A0A0C2RMP5;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=C5a peptidase {ECO:0000256|ARBA:ARBA00020956};
DE EC=3.4.21.110 {ECO:0000256|ARBA:ARBA00012942};
DE AltName: Full=SCP {ECO:0000256|ARBA:ARBA00030432};
GN ORFNames=KR50_34170 {ECO:0000313|EMBL:KIL43014.1};
OS Jeotgalibacillus campisalis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae; Jeotgalibacillus.
OX NCBI_TaxID=220754 {ECO:0000313|EMBL:KIL43014.1, ECO:0000313|Proteomes:UP000031972};
RN [1] {ECO:0000313|EMBL:KIL43014.1, ECO:0000313|Proteomes:UP000031972}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SF-57 {ECO:0000313|EMBL:KIL43014.1,
RC ECO:0000313|Proteomes:UP000031972};
RA Goh K.M., Chan K.-G., Yaakop A.S., Ee R., Gan H.M., Chan C.S.;
RT "Jeotgalibacillus campisalis genome sequencing.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This virulence factor of S.pyogenes specifically cleaves the
CC human serum chemotaxin C5a at '68-Lys-|-Asp-69' bond near its C-
CC terminus, destroying its ability to serve as a chemoattractant.
CC {ECO:0000256|ARBA:ARBA00002909}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=The primary cleavage site is at 67-His-|-Lys-68 in human C5a
CC with a minor secondary cleavage site at 58-Ala-|-Ser-59.;
CC EC=3.4.21.110; Evidence={ECO:0000256|ARBA:ARBA00001404};
CC -!- SIMILARITY: Belongs to the peptidase S8 family.
CC {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIL43014.1}.
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DR EMBL; JXRR01000022; KIL43014.1; -; Genomic_DNA.
DR RefSeq; WP_041061215.1; NZ_JXRR01000022.1.
DR PATRIC; fig|220754.4.peg.3432; -.
DR OrthoDB; 9798386at2; -.
DR Proteomes; UP000031972; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd07475; Peptidases_S8_C5a_Peptidase; 1.
DR Gene3D; 3.40.50.12090; -; 2.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR Gene3D; 2.60.40.1710; Subtilisin-like superfamily; 1.
DR InterPro; IPR034216; C5a_Peptidase.
DR InterPro; IPR007253; Cell_wall-bd_2.
DR InterPro; IPR010435; Fn3_5.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR PANTHER; PTHR43399; SUBTILISIN-RELATED; 1.
DR PANTHER; PTHR43399:SF4; TK-SUBTILISIN; 1.
DR Pfam; PF04122; CW_binding_2; 3.
DR Pfam; PF06280; fn3_5; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000031972};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Secreted {ECO:0000256|ARBA:ARBA00022512};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 28..1543
FT /note="C5a peptidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002154498"
FT DOMAIN 201..663
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 459..532
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT DOMAIN 687..813
FT /note="Fn3-like"
FT /evidence="ECO:0000259|Pfam:PF06280"
FT ACT_SITE 210
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 276
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 601
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 1543 AA; 165703 MW; B2916D5476BA8784 CRC64;
MTKKWKNNVT AALALSLVLG NSSVALGQDN QNDAPKKDAN GEVPVNVLSN AQAKMAKLDQ
KAKVTADKAA LKESDMVRVI VEVAGETAIE HATKKGVLYK ELSDSEKENF EQEAASSQQS
VKQSISSKGV DIEYKNSFSA SVNGFSGIIQ YKDFGAIESL PGVKKVYIAN EYERPEFEED
MVTSHEFIQS YQTWADAKYK GEGMIVSVID SGTDVDHRDF VLSDGVEGEL TKEEVSALKD
ENSVTGNFYN EKVPYAYNYY DLNDTVEDLG PDASMHGMHV AGTVAANGDT ENGGIKGVAP
EAQILGMKVF SNDPNFASTY SDIYLKAIDD SIKLGADVLN MSLGSTASFY EEESPEDLAI
TRAVENGIVS TVSAGNSGHI GRGYSNPHYE NPDIGLVGAP GLNADTLQVA ASGNYMDLYT
HSVNGEGVPE FKGYGIDDWS KLGEVEVVSL KALSGNSGAL GAPADYSGID VKGKVVVVER
GGLPFVDKTV NAANAGAAGI IVYNSTSPVF YKDQGGWDIP FMKVERAAGL DLEAVLADGS
SLMMDVTQTA KEEGPEVGRA TDFTSWGVTP DLEFKPEITA PGGGILSTVN DDEYTVMSGT
SMAAPHVAGG SALVQQYLLD DDRFNEYNSD ERTRLAKALL MNTADIIHDL NGQPFSPRRQ
GAGMMQTYAA VSTPVFVTDS ETNEAKVNLK DFTSKTVSMT LTATNLTGED AEYNVDTSVL
ADTFVKDGDS TYNALVAGDL EGAVVSGPES VTVPANGSVD VTVSIDLTNA KVKGIDEDGN
EELIDLPADQ FVEGFVKLVS TDLENPDLAV PYLGFYGKWE NPDIVDNMSF EGEDRFYNTP
SSVVQLNREN IPTFSSGIEV DGEIVYPLST KNKDGLYDDI NVAPSFLRNA KEVQFNVLNG
ENTKLKTISL ENNIRKNYAA AGQYSYSGAR VWNSSVKDKI VDDGMYFYEI KSRVDYDGAK
WQSKKVPVYV DNTAPEVNDV SFDKDTNTLS FTGVDEGVGI LWYDLYINGQ YVTPTEKLNG
DTTSVNLSNY GFSAEEIQSL EIVPVDKAGN VGYEASFVDD GDAPIIYLDD NAPIALNYYK
TRTIPVAGKI VEENALESVK VNGKEVPFTP STDGFVFSTE VTVESDGKHD ITIEATDVNG
NSTSIARGFW VDTVAPQVVV NTDELVNNST DQVEVDVLLS DNFNGVELSL EDDVLFSSMN
TTSDETKPFE GTVPVTLDLE EGSNTFEFKA IDTAGNETVY ELSITRNDSG LFVERISGKN
RYETAVELSQ TEWDSADTVI LARGDNYADA LAGVPLTSMH DGPLLLSRTA ALPSSTLDEI
KRLGAKKVVV LGGEGAVSAD VVKTLKNQGI TVERISGKDR YETAAKIAEK FGKSSNAVVV
NGQNFPDALS AASYAAQQKM PILLTKSSSL PKATKDALAK LEANRTFVVG GSGVVSNEVM
GQVPNAHRIS GKDRYATSLE VAKFFGQDSD TVYVATGRDF ADALSGSAVA AKSGSGLYLV
GSSVSDDLGQ HLQDEDFNYA KVIGGKTIVS EDIVQQLNDF LEE
//
