ID A0A0C4WK04_9GAMM Unreviewed; 405 AA.
AC A0A0C4WK04;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=General secretion pathway protein F {ECO:0000256|ARBA:ARBA00030750};
GN Name=gspF {ECO:0000313|EMBL:AJE22998.1};
GN ORFNames=Achr_36030 {ECO:0000313|EMBL:AJE22998.1};
OS Azotobacter chroococcum NCIMB 8003.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Azotobacter.
OX NCBI_TaxID=1328314 {ECO:0000313|EMBL:AJE22998.1, ECO:0000313|Proteomes:UP000068210};
RN [1] {ECO:0000313|EMBL:AJE22998.1, ECO:0000313|Proteomes:UP000068210}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCIMB 8003 {ECO:0000313|EMBL:AJE22998.1,
RC ECO:0000313|Proteomes:UP000068210};
RX PubMed=26061173;
RA Robson R.L., Jones R., Robson R.M., Schwartz A., Richardson T.H.;
RT "Azotobacter Genomes: The Genome of Azotobacter chroococcum NCIMB 8003
RT (ATCC 4412).";
RL PLoS ONE 10:E0127997-E0127997(2015).
CC -!- FUNCTION: Component of the type II secretion system inner membrane
CC complex required for the energy-dependent secretion of extracellular
CC factors such as proteases and toxins from the periplasm.
CC {ECO:0000256|ARBA:ARBA00002684}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429, ECO:0000256|RuleBase:RU003923}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004429,
CC ECO:0000256|RuleBase:RU003923}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the GSP F family.
CC {ECO:0000256|ARBA:ARBA00005745, ECO:0000256|RuleBase:RU003923}.
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DR EMBL; CP010415; AJE22998.1; -; Genomic_DNA.
DR RefSeq; WP_039806307.1; NZ_CP010415.1.
DR AlphaFoldDB; A0A0C4WK04; -.
DR STRING; 1328314.Achr_36030; -.
DR KEGG; acx:Achr_36030; -.
DR HOGENOM; CLU_035032_0_1_6; -.
DR Proteomes; UP000068210; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR Gene3D; 1.20.81.30; Type II secretion system (T2SS), domain F; 2.
DR InterPro; IPR003004; GspF/PilC.
DR InterPro; IPR011850; T2SS_GspF.
DR InterPro; IPR001992; T2SS_GspF/T4SS_PilC_CS.
DR InterPro; IPR018076; T2SS_GspF_dom.
DR InterPro; IPR042094; T2SS_GspF_sf.
DR NCBIfam; TIGR02120; GspF; 1.
DR PANTHER; PTHR30012; GENERAL SECRETION PATHWAY PROTEIN; 1.
DR PANTHER; PTHR30012:SF0; TYPE II SECRETION SYSTEM PROTEIN F-RELATED; 1.
DR Pfam; PF00482; T2SSF; 2.
DR PRINTS; PR00812; BCTERIALGSPF.
DR PROSITE; PS00874; T2SP_F; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000068210};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU003923};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU003923}.
FT TRANSMEM 168..192
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 223..242
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 376..397
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 71..193
FT /note="Type II secretion system protein GspF"
FT /evidence="ECO:0000259|Pfam:PF00482"
FT DOMAIN 273..395
FT /note="Type II secretion system protein GspF"
FT /evidence="ECO:0000259|Pfam:PF00482"
SQ SEQUENCE 405 AA; 44435 MW; BA60EF9D6294E048 CRC64;
MAAFSYRALT TDGKTRNGLI EADSPRHARA MLRERGLLPT AVEAAGPARA TRTGKFRLVR
PLPARDVALL TRQLATLIDS GLPLEEALYA ITRQTSNALL KATVLSVRAR ILEGHSLASA
LSEYPRSFNE LYRAMITAGE ESGHLSGVLL RLAEYGERLQ KIRSKVQVAL IYPVFLTLVA
VGVIVLLLTY VVPKVVEQFT HAEQTLPLLT RYLILISDVL QQYGLHMLAA LAGLALLARW
WLSDGGRRTR WHKAQLRLPL VSTFVLGQQS LQFARTLAIL VGSGIDLLHS LKVSAGPMTN
LYLKQAVVNT GDLVREGCSL SRALEQSGPF PPLLVYMIAN GERSGELEKM LDKAAENQEA
EFESRVAWLL GLFEPALILV MGGIVLCIVL AILMPILELN NLTSF
//
